抗Growth Hormone抗体(Anti-Growth Hormone, Human, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human, Mouse, Rat
Label	Unconjugated
Immunogen	E. coli-derived recombinant human Growth HormonePhe27-Phe217Accession # CAA23779
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects human Growth Hormone in direct ELISAs and Western blots.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.5 µg/mL	See below
ELISA	This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human Growth Hormone Monoclonal Antibody (Catalog # MAB10671). This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human Growth Hormone (GH) DuoSet ELISA Kit (Catalog # DY1067) for convenient development of a sandwich ELISA or the Human Growth Hormone Quantikine ELISA Kit (Catalog # DGH00) for a complete optimized ELISA.
Immunohistochemistry	5-15 µg/mL	See below
Neutralization	Measured by its ability to neutralize Growth Hormone-induced proliferation in the Nb2‑11 rat lymphoma cell line. Gout, P.W. et al. (1980) Cancer Res. 40:2433. The Neutralization Dose (ND50) is typically 1.5-7.5 ng/mL in the presence of 0.2 ng/mL Recombinant Human Growth Hormone.

Western Blot
	Detection of Human, Mouse, and Rat Growth Hormone by Western Blot. Western blot shows lysates of human, mouse, and rat pituitary gland tissue. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human Growth Hormone Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1067) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Growth Hormone at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunohistochemistry
	Growth Hormone in Human Pituitary. Growth Hormone was detected in immersion fixed paraffin-embedded sections of human pituitary using Goat Anti-Human/Mouse/Rat Growth Hormone Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1067) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Neutralization
	Cell Proliferation Induced by Growth Hormone and Neutralization by Human Growth Hormone Antibody. Recombinant Human Growth Hormone (Catalog # 1067-GH) stimulates proliferation in the Nb2‑11 rat lymphoma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human Growth Hormone (0.2 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human/Mouse/Rat Growth Hormone Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1067). The ND50 is typically 1.5-7.5 ng/mL.
ELISA
	Human Growth Hormone ELISA Standard Curve. Recombinant Human Growth Hormone protein was serially diluted 2-fold and captured by Mouse Anti-Human Growth Hormone Monoclonal Antibody (Catalog # MAB10671) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human/Mouse/Rat Growth Hormone Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1067) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

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Related Product & Information

Entrez Gene IDs	2688 (Human)
Background	Growth Hormone
background_content	Background: Growth Hormone Growth Hormone (GH), also known as somatotropin, is a member of a family of growth factors that includes prolactin, placental lactogens, proliferins, and somatolactin (1, 2). It is synthesized primarily by somatotropes in the anterior pituitary and is stored in secretary granules. The pulsatile release of GH into circulation is regulated by the concerted actions of the hypothalamic hormones - GH-releasing hormone (GHRH) and somatostatin (SST) - as well as by signals from the periphery - ghrelin (3) and leptin (4). The human GH cDNA encodes a 217 amino acid (aa) residue precursor protein with a 26 aa putative signal peptide. By alternative splicing, at least four isoforms of GH have been identified (5). Human GH is a pleiotropic cytokine that exerts its biological actions by binding to the transmembrane GH receptor, which is present in many cell types (1, 2). GH stimulates the liver and other tissues to produce IGF-I, which regulates growth and metabolism. GH has also been shown to have direct effects on growth that is independent of IGF-I. GH, directly or indirectly via IGF-I, can act on B cells, T cells, NK cells, macrophages, and neutrophils to exert immunomodulatory activities (6). In addition, GH can act directly on various cell types to induce lipolysis, lactation, amino acid uptake, and protein synthesis (1, 2, 6).

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Citations

1. PDK1-FoxO1 pathway in AgRP neurons of arcuate nucleus promotes bone formation via GHRH-GH-IGF1 axis
   Authors: H Sasanuma, M Nakata, K Parmila, J Nakae, T Yada
   Mol Metab, 2017;6(5):428-439.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC paraffin embedded


2. Growth hormone is permissive for neoplastic colon growth
   Proc Natl Acad Sci USA, 2016;0(0):.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


3. STAT3 upregulation in pituitary somatotroph adenomas induces growth hormone hypersecretion.
   Authors: Zhou, Cuiqi, Jiao, Yonghui, Wang, Renzhi, Ren, Song-Gua, Wawrowsky, Kolja, Melmed, Shlomo
   J Clin Invest, 2015;125(4):1692-702.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


4. Troubleshooting PEG-hGH detection supporting pharmacokinetic evaluation in growth hormone deficient patients.
   Authors: Myler HA, McVay S, Kratzsch J
   J Pharmacol Toxicol Methods, 2010;61(2):92-7.
   Species: Human
   Sample Type: Serum
   Application: Electrochemiluminescent Assay


5. Growth hormone is present in the human retina and vitreous fluid.
   Authors: Harvey S, Parker E, Macdonald I, Sanders EJ
   Neurosci. Lett., 2009;455(3):199-202.
   Species: Human
   Sample Type: Vitreous Humor
   Application: WB


6. Shift of monocyte function toward cellular immunity during sleep.
   Authors: Lange T, Dimitrov S, Fehm HL, Westermann J, Born J
   Arch. Intern. Med., 2006;166(16):1695-700.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut

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