抗ST2L抗体(Anti-ST2L, Mouse, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

目次に戻る

Product Details

Species Reactivity	Mouse
Label	Unconjugated
Immunogen	S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse ST2/IL-33 RSer27-Arg332Accession # P14719
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects mouse ST2/IL-33 R in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human ST2/IL-33 R is observed.

目次に戻る

Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Mouse ST2/IL-33 R Fc Chimera (Catalog # 1004-MR)
Neutralization	Measured by its ability to neutralize IL‑33-induced proliferation in the D10.G4.1 mouse helper T cell line. Schmitz, J. et al. (2005) Immunity 23:479. The Neutralization Dose (ND50) is typically 1-6 μg/mL in the presence of 1 ng/mL Recombinant Human IL‑33 and Hamster Anti-Mouse CD3 epsilon Monoclonal Antibody.

Neutralization

Cell Proliferation Induced by IL‑33 and Neutralization by Mouse ST2/IL-33 R Antibody. Recombinant Human IL‑33 (Catalog # 3625-IL) induces proliferation in the D10.G4.1 mouse helper T cell line in the presence of Hamster Anti-Mouse CD3 epsilon Monoclonal Antibody (Catalog # MAB484) in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Under these conditions, proliferation elicited by IL‑33 is neutralized (green line) by increasing concentrations of Goat Anti-Mouse ST2/IL-33 R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1004). The ND50 is typically1-6 μg/mL.

目次に戻る

Related Product & Information

Long Name	Interleukin 33 Receptor
Entrez Gene IDs	9173 (Human); 17082 (Mouse)
Background	ST2/IL-33 R
background_content	Background: ST2/IL-33 R ST2, also known as IL-1 R4 and T1, is an Interleukin-1 receptor family glycoprotein that contributes to Th2 immune responses. Mouse ST2 consists of a 306 amino acid (aa) extracellular domain (ECD) with three Ig-like domains, a 23 aa transmembrane segment, and a 212 aa cytoplasmic domain with an intracellular TIR domain. Alternate splicing of the 120 kDa mouse ST2 generates a soluble 60 kDa isoform that lacks the transmembrane and cytoplasmic regions. Within the ECD, mouse ST2 shares 68% and 81% aa sequence identity with human and rat ST2, respectively. ST2 is expressed on the surface of mast cells, activated Th2 cells, macrophages, and cardiac myocytes. It binds IL-33, a cytokine that is upregulated by inflammation or mechanical strain in smooth muscle cells, airway epithelia, keratinocytes, and cardiac fibroblasts. IL-33 binding induces the association of ST2 with IL-1R AcP, a shared signaling subunit that also associates with IL-1 RI and IL‑1 R rp2. In macrophages, ST2 interferes with signaling from IL-1 RI and TLR4 by sequestering the adaptor proteins MyD88 and Mal. In addition to its role in promoting mast cell and Th2 dependent inflammation, ST2 activation enhances antigen induced hypernociception and protects from atherosclerosis and cardiac hypertrophy. The soluble ST2 isoform is released by activated Th2 cells and strained cardiac myocytes and is elevated in the serum in allergic asthma. Soluble ST2 functions as a decoy receptor that blocks IL-33’s ability to signal through transmembrane ST2.

目次に戻る

Citations

1. alpha-Galactosylceramide but not phenyl-glycolipids induced NKT cell anergy and IL-33-mediated myeloid-derived suppressor cell accumulation via upregulation of egr2/3.
   Authors: Huang J, Tsai Y, Chang Y, Wu J, Hung J, Lin K, Wong C, Yu A
   J Immunol, 2014;192(4):1972-81.
   Species: Mouse
   Sample Type: In Vivo
   Application: Neut


2. Interleukin-33 and alveolar macrophages contribute to the mechanisms underlying the exacerbation of IgE-mediated airway inflammation and remodelling in mice.
   Authors: Mizutani N, Nabe T, Yoshino S
   Immunology, 2013;139(2):205-18.
   Species: Mouse
   Sample Type: In Vivo
   Application: In vivo


3. IL-33 is produced by mast cells and regulates IgE-dependent inflammation.
   Authors: Hsu CL, Neilsen CV, Bryce PJ
   PLoS ONE, 2010;5(8):e11944.
   Species: Mouse
   Sample Type: In Vivo
   Application: In vivo


4. Protective effect of IL-18 on kainate- and IL-1beta-induced cerebellar ataxia in mice.
   Authors: Andoh T, Kishi H, Motoki K, Nakanishi K, Kuraishi Y, Muraguchi A
   J. Immunol., 2008;180(4):2322-8.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC Frozen


5. ST2 is essential for Th2 responsiveness and resistance to pseudomonas aeruginosa keratitis.
   Authors: Huang X, Du W, Barrett RP, Hazlett LD
   Invest. Ophthalmol. Vis. Sci., 2007;48(10):4626-33.
   Species: Mouse
   Sample Type: Tissue Homogenates
   Application: WB

目次に戻る