抗HCN4モノクローナル抗体(Anti-HCN4) HCN4 antibody (S114-10:クローン名) | StressMarq Biosciences

Western blot analysis of HCN4 in T-HEK cells using a 1:1000 dilution of SMC-320.

IHC analysis of HCN4 in frozen sections of mouse brain extracts using SMC-320.

HCN4 Antibody

Monoclonal

Anti-HCN4

Ion Channels, Neuroscience

Hyperpolarization activated cyclic nucleotide gated potassium channel 4, HCN 4, Potassium/sodium hyperpolarization activated cyclic nucleotide gated channel 4

S114-10

Mouse

IgG1

Fusion protein amino acids 1019-1198 (c-terminus) of rat HCN4, accession number Q9JKA7

WB, ICC, IHC, IF

Human, Mouse, Rat

NP_067690.1

59266

Q9JKA7

Detects ~130kDa. No cross-reactivity against other HCNs

1-10ug/mL (WB), 0.1-1.0ug/mL (Perox) (IHC/ICC)

Protein G Purified

PBS pH7.4, 50% glycerol, 0.09% sodium azide

1 µg/mL of SMC-320 was sufficient for detection of HCN4 in 10µg of COS cell lysate transiently transfected with HCN4 by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Research Background
Ion channels are integral membrane proteins that help establish and control the small voltage gradient across the plasma membrane of living cells by allowing the flow of ions down their electrochemical gradient (1).   They are present in the membranes that surround all biological cells because their main function is to regulate the flow of ions across this membrane.  Whereas some ion channels permit the passage of ions based on charge, others conduct based on a ionic species, such as sodium or potassium.  Furthermore, in some ion channels, the passage is governed by a gate which is controlled by chemical or electrical signals, temperature, or mechanical forces. There are a few main classifications of gated ion channels.  There are voltage- gated ion channels, ligand- gated, other gating systems and finally those that are classified differently, having more exotic characteristics.  The first are voltage- gated ion channels which open and close in response to membrane potential.  These are then separated into sodium, calcium, potassium, proton, transient receptor, and cyclic nucleotide-gated channels; each of which is responsible for a unique role.  Ligand-gated ion channels are also known as ionotropic receptors, and they open in response to specific ligand molecules binding to the extracellular domain of the receptor protein.  The other gated classifications include activation and inactivation by second messengers, inward-rectifier potassium channels, calcium-activated potassium channels, two-pore-domain potassium channels, light-gated channels, mechano-sensitive ion channels and cyclic nucleotide-gated channels.  Finally, the other classifications are based on less normal characteristics such as two-pore channels, and transient receptor potential channels (2). Specifically, hyperpolarization-activated cation channels of the HCN gene family contribute to spontaneous rhythmic activity in both the heart and brain (3).

References
1. Hille B. (2001) Ion Channels of Excitable Membranes, 3rd Ed., Sinauer Associated Inc.:Sunderland, MA USA.

2. www.iochannels.org

3. Zong X., et al. (2005) J Biol Chem. 280(40): 34224-34232