抗POU2AF1, Mouse-Mono(UMAB219) UltraMAB抗体 | Anti-POU2AF1, Mouse-Mono(UMAB219) UltraMAB Antibody

• 価格
• 特長
• 使用例
• 製品情報

価格

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特長

UltraMAB抗体について
OriGene社製品の中でも、特にUltraMAB抗体シリーズは、ヒト組織の免疫染色(IHC)に適した非常に特異性の高いモノクローナル抗体です。抗原にはTrueMAB抗体と同様、組換え体ヒト全長タンパク質を使用しています。1万種類を超えるタンパク質をスポットしたマイクロアレイを用い、標的タンパク質以外の分子との交差反応がないことを確認済みの、癌研究・病理学・解剖学向けの製品です。

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使用例

	Immunohistochemical staining of paraffin-embedded normal spleen using mouse anti-POU2AF1 clone UMAB219 (UM800111) at 1:200 with Polink2 Broad HRP DAB detection kit; pretreatment of tissue prior to stain with heat-induced epitope retrieval with EDTA pH 8.0 buffer using pressure chamber for 3 minutes at 110C is required for optimal staining. Cells exhibit cytoplamic and membranous staining .
	Immunohistochemical staining of paraffin-embedded human lymphoma tissue using anti-POU2AF1 mouse monoclonal antibody. HIER pretreatment was done with 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 minutes. UM800111 was diluted 1:250 and detection was done with HRP secondary and DAB chromogen. Strong nuclear, cytoplasmic, and membraneous staining tumor cells.
	Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-POU2AF1 mouse monoclonal antibody. HIER pretreatment was done with 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 minutes. UM800111 was diluted 1:250 and detection was done with HRP secondary and DAB chromogen. Strong nuclear, cytoplasmic, and membraneous staining germinal cells of the tonsil.
	Immunohistochemical staining of paraffin-embedded tonsil using mouse anti-POU2AF1 clone UMAB219 (UM800111) at 1:200 with Polink2 Broad HRP DAB detection kit; pretreatment of tissue prior to stain with heat-induced epitope retrieval with EDTA pH 8.0 buffer using pressure chamber for 3 minutes at 110C is required for optimal staining. Cells exhibit cytoplamic and membranous staining .
	OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-POU2AF1 mouse monoclonal antibody (UM800111). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification.(1:100)

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製品情報

Clone Name	UMAB219
Applications	IHC, 10K-CHIP
Recommend Dilution	IHC 1:100~250,
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Full length human recombinant protein of human POU2AF1(NP_006226) produced in E.coli.
Isotype	IgG2b
Formulation	PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Concentration	0.5~1.0 mg/ml (Lot Dependent)
Purification	Purified from cell culture supernatant by affinity chromatography
Predicted Protein Size	27.3 kDa
Gene Name	Homo sapiens POU class 2 associating factor 1 (POU2AF1)
Background	Transcriptional coactivator that specifically associates with either OCT1 or OCT2. It boosts the OCT1 mediated promoter activity and to a lesser extent, that of OCT2. It has no intrinsic DNA-binding activity. It recognizes the POU domains of OCT1 and OCT2. It is essential for the response of B-cells to antigens and required for the formation of germinal centers. [UniProtKB/Swiss-Prot Function]
Synonyms	BOB1; OBF-1; OBF1; OCAB
Database Link	NP_006226

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