抗TP53, Mouse-Mono(UMAB206) UltraMAB抗体 | Anti-TP53, Mouse-Mono(UMAB206) UltraMAB Antibody

• 価格
• 特長
• 使用例
• 製品情報

価格

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特長

UltraMAB抗体について
OriGene社製品の中でも、特にUltraMAB抗体シリーズは、ヒト組織の免疫染色(IHC)に適した非常に特異性の高いモノクローナル抗体です。抗原にはTrueMAB抗体と同様、組換え体ヒト全長タンパク質を使用しています。1万種類を超えるタンパク質をスポットしたマイクロアレイを用い、標的タンパク質以外の分子との交差反応がないことを確認済みの、癌研究・病理学・解剖学向けの製品です。

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使用例

	Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-TP53 Mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120C for 3 min, UM800098)(1:200)
	Western blot analysis of extracts (25ug) from 4 different cell lines by using anti-TP53 monoclonal antibody. (1:500) (1: HEK293; 2: Hela; 3: MCF7; 4: HepG2) Dilution: 1:500
	Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-TP53 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120C for 3 min, UM800098)(1:200)
	Immunofluorescent staining of HeLa cells using anti-TP53 mouse monoclonal antibody (UM800098, green, 1:100). Actin filaments were labeled with Alexa Fluor® 594 Phalloidin (red), and nuclear with DAPI (blue).
	OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-TP53 mouse monoclonal antibody (UM800098). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification.(1:100)

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製品情報

Clone Name	UMAB206
Applications	WB, IHC, IF, 10K-CHIP
Recommend Dilution	IHC 1:100~200,
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Full length human recombinant protein of human TP53 (NP_000537) produced in SF9 cell.
Isotype	IgG1
Formulation	PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Concentration	0.5~1.0 mg/ml (Lot Dependent)
Purification	Purified from cell culture supernatant by affinity chromatography
Predicted Protein Size	44.1 kDa
Gene Name	Homo sapiens tumor protein p53 (TP53), transcript variant 1
Background	This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons (PMIDs: 12032546, 20937277). [provided by RefSeq, Feb 2013].
Synonyms	BCC7; LFS1; P53; TRP53
Database Link	NP_000537

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