抗PD-1 (PDCD1), Mouse-Mono(UMAB197) UltraMAB抗体 | Anti-PD-1 (PDCD1), Mouse-Mono(UMAB197) UltraMAB Antibody

• 価格
• 特長
• 使用例
• 製品情報

価格

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特長

UltraMAB抗体について
OriGene社製品の中でも、特にUltraMAB抗体シリーズは、ヒト組織の免疫染色(IHC)に適した非常に特異性の高いモノクローナル抗体です。抗原にはTrueMAB抗体と同様、組換え体ヒト全長タンパク質を使用しています。1万種類を超えるタンパク質をスポットしたマイクロアレイを用い、標的タンパク質以外の分子との交差反応がないことを確認済みの、癌研究・病理学・解剖学向けの製品です。

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使用例

	Immunohistochemical staining of paraffin-embedded Human tonsil using anti-PDCD1 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120C for 3 min, UM800089)(1:200)
	Western blot of cell lysates (35ug) from 9 different cell lines (1: HepG2, 2: HeLa, 3: SV-T2, 4: A549. 5: COS7, 6: Jurkat, 7: MDCK, 8: PC-12, 9: MCF7).
	Western blot of human tissue lysates (15ug) from 10 different tissues (1: Testis, 2: Omentum, 3: Uterus, 4: Breast, 5: Brain, 6: Liver, 7: Ovary, 8: Thyroid, 9: Colon, 10: Spleen). Diluation: 1:500.
	Immunofluorescent staining of PDCD1(RC210364)-stable-expression cells (left) labeling PDCD1 with mouse monoclonal antibody UM800089 (1:100, green) and nucleus with Hoechst33342 (blue) . HEK293T cells serve as negative control (right).
	Immunocytochemistry staining of PDCD1(RC210364)-stable-expression cells (left) labeling PDCD1 with mouse anti-PDCD1 monoclonal antibody UM800089 (1:900). The rihgt is negative control.

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製品情報

Clone Name	UMAB197
Applications	WB, IHC, IF
Recommend Dilution	IHC 1:200, WB 1:500, IF 1:100..
Reactivity	Human, Mouse, Monkey, Dog, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Full length human recombinant protein of human PDCD1 (NP_005009) produced in HEK293T cell.
Isotype	IgG2a
Formulation	PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Concentration	0.5~1.0 mg/ml (Lot Dependent)
Purification	Purified from cell culture supernatant by affinity chromatography
Predicted Protein Size	29.2 kDa
Gene Name	Homo sapiens programmed cell death 1 (PDCD1)
Background	This gene encodes a cell surface membrane protein of the immunoglobulin superfamily. This protein is expressed in pro-B-cells and is thought to play a role in their differentiation. In mice, expression of this gene is induced in the thymus when anti-CD3 antibodies are injected and large numbers of thymocytes undergo apoptosis. Mice deficient for this gene bred on a BALB/c background developed dilated cardiomyopathy and died from congestive heart failure. These studies suggest that this gene product may also be important in T cell function and contribute to the prevention of autoimmune diseases. [provided by RefSeq, Jul
Synonyms	CD279; hPD-1; hPD-l; hSLE1; PD-1; PD1; SLEB2
Database Link	NP_005009

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