抗GEMIN8, Mouse-Mono(UMAB185) UltraMAB抗体 | Anti-GEMIN8, Mouse-Mono(UMAB185) UltraMAB Antibody

• 価格
• 特長
• 使用例
• 製品情報

価格

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特長

UltraMAB抗体について
OriGene社製品の中でも、特にUltraMAB抗体シリーズは、ヒト組織の免疫染色(IHC)に適した非常に特異性の高いモノクローナル抗体です。抗原にはTrueMAB抗体と同様、組換え体ヒト全長タンパク質を使用しています。1万種類を超えるタンパク質をスポットしたマイクロアレイを用い、標的タンパク質以外の分子との交差反応がないことを確認済みの、癌研究・病理学・解剖学向けの製品です。

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使用例

	Immunohistochemical staining of paraffin-embedded human pancreas using anti- GEMIN8 clone UMAB185 mouse monoclonal antibody at 1:400 dilution of 1.0 mg/mL using Polink2 Broad HRP DAB for detection. UM800077 requires HIER with with Accel 3in1 EDTA solution ph8.7 at 110C for 3 min using pressure chamber/cooker. The shows strong membraneous and cytoplasmic staining in exocrin cells.
	Western blot analysis of extracts (15ug) from 9 Human tissue by using anti-GEMIN8 monoclonal antibody (1: Testis; 2: Uterus; 3: Breast; 4: Brain; 5: Liver; 6: Ovary; 7: Thyroid gland; 8: colon:;9:Spleen).(1:500) Dilution: 1:500
	Western blot of cell lysates (35ug) from 9 different cell lines (1: HepG2, 2: HeLa, 3: SV-T2, 4: A549, 5: COS7, 6: Jurkat, 7: MDCK, 8: PC-12, 9: MCF7). Diluation: 1:500
	Immunohistochemical staining of paraffin-embedded Human liver tissue using anti-GEMIN8 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 110C for 10 min, UM800077)(1:400)
	Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-GEMIN8 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 110C for 10 min, UM800077)(1:400)
	Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-GEMIN8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 110C for 10 min, UM800077)(1:400)
	Immunohistochemical staining of paraffin-embedded human kidney using anti- GEMIN8 clone UMAB185 mouse monoclonal antibody at 1:400 dilution of 1.0 mg/mL using Polink2 Broad HRP DAB for detection. UM800077 requires HIER with with Accel 3in1 EDTA solution ph8.7 at 110C for 3 min using pressure chamber/cooker. The tubule epithelia cells shows strong membraneous and cytoplasmic staining.
	OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-GEMIN8 mouse monoclonal antibody (UM800077). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification.(1:100)

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製品情報

Clone Name	UMAB185
Applications	WB, IHC, 10K-CHIP
Recommend Dilution	IHC 1:100~400,
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Full length human recombinant protein of human GEMIN8 (NP_060326) produced in E.coli.
Isotype	IgG1
Formulation	PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Concentration	0.5~1.0 mg/ml (Lot Dependent)
Purification	Purified from cell culture supernatant by affinity chromatography
Predicted Protein Size	28.5 kDa
Gene Name	Homo sapiens gem nuclear organelle associated protein 8 (GEMIN8), transcript variant 3
Background	The protein encoded by this gene is part of the SMN complex, which is necessary for spliceosomal snRNP assembly in the cytoplasm and pre-mRNA splicing in the nucleus. The encoded protein binds to both SMN1 and the GEMIN6/GEMIN7 heterodimer, mediating their interaction. This protein is found in nuclear Gemini of Cajal bodies (gems) and in the cytoplasm. Three transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, May 2010].
Synonyms	FAM51A1; FAM51A1
Database Link	NP_060326

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