抗Rb [SPM353]抗体 | Anti-Rb [SPM353] antibody

• 特長
• 価格
• DATA IMAGES
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

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DATA IMAGES

IHC-P analysis of human colon tissue using GTX34994 Rb antibody [SPM353].

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製品情報

Host	Mouse
Clonality	Monoclonal
Clone Name	SPM353
Isotype	IgG1
Application	WB, IHC-P, IP
Reactivity	Human, Mouse

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APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1-2μg/ml
IHC-P	1-2μg/ml for 30 minutes at RT
IP	0.5-1μg/500ug protein lysate
Note : IHC-P Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.

Not tested in other applications.

Calculated MW	106 kDa. ( Note )
Specificity/Sensitivity	Recognizes a 105kDa phosphoprotein, identified as retinoblastoma (Rb) gene product. Its epitope is localized between aa 703-772. It shows no cross reaction with p107 or p130.

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PROPERTIES

Form	Liquid
Buffer	10mM PBS, 0.05% BSA, 0.05% sodium azide (Please contact us for PBS only format)
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration	0.2 mg/ml
Antigen Species	Human
Immunogen	Recombinant human Rb1 protein fragment of 283 amino acid residues.
Purification	Protein A/G purified
Conjugation	Unconjugated
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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TARGET

Synonyms	OSRC,PPP1R130,RB,RB transcriptional corepressor 1,RB1,p105Rb,pRb,pp110,Rb
Cellular Localization	Nucleus
Background	The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma. [provided by RefSeq, Jul 2008]
Database	・ Gene ID: 5925 RB1 ・ UniProt: P06400 RB1

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