抗Cyclin D1 [DCS-6]抗体 | Anti-Cyclin D1 [DCS-6] antibody

• 特長
• 価格
• DATA IMAGES
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET
• REFERENCEE(抜粋)

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

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DATA IMAGES

	IHC-P analysis of human mantle cell lymphoma tissue using GTX34618 Cyclin D1 antibody [DCS-6].
	FACS analysis of Jurkat cells using GTX34618 Cyclin D1 antibody [DCS-6]. Black : BlankGreen : Isotype ControlRed : Primary antibody
	WB analysis of (1) C2C12, (2) HepG2, & (3) NIH3T3 cell lysates using GTX34618 Cyclin D1 antibody [DCS-6].

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製品情報

Host	Mouse
Clonality	Monoclonal
Clone Name	DCS-6
Isotype	IgG2a
Application	WB, ICC/IF, IHC-P, FACS
Reactivity	Human, Mouse, Rat, Monkey

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APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1-2μg/ml
ICC/IF	1-2μg/ml
IHC-P	1-2μg/ml for 30 minutes at RT
FACS	1-2μg/10⁶ cells
Note : IHC-P Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.

Not tested in other applications.

Calculated MW	34 kDa. ( Note )

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PROPERTIES

Form	Liquid
Buffer	10mM PBS, 0.05% BSA, 0.05% sodium azide (Please contact us for PBS only format)
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration	0.2 mg/ml
Antigen Species	Human
Immunogen	Human full length recombinant cyclin D1 protein
Purification	Protein A/G purified
Conjugation	Unconjugated
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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TARGET

Synonyms	BCL1,CCND1,D11S287E,PRAD1,U21B31,cyclin D1,Cyclin D1
Cellular Localization	Nucleus,Cytoplasm,Membrane
Background	The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis. [provided by RefSeq, Jul 2008]
Database	・ Gene ID: 595 CCND1 ・ UniProt: P24385 CCND1

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REFERENCEE(抜粋)

Application Reference	Application/Reactivity
Wang DW et al. Eur. Rev. Med. Pharmacol. Sci. 2018; 22 : 1241-1248 MiR-16 inhibits pituitary adenoma cell proliferation via the suppression of ERK/MAPK signal pathway.	Application : WB Reactivity : Human

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