抗CD27 [LPFS2/1611]抗体 | Anti-CD27 [LPFS2/1611] antibody

• 特長
• 価格
• DATA IMAGES
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

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DATA IMAGES

	FACS analysis of Ramos cells using GTX34479 CD27 antibody [LPFS2/1611]. Blue : Primary antibodyRed : Isotype control
	ICC/IF analysis of Ramos cells using GTX34479 CD27 antibody [LPFS2/1611]. Green : Primary antibodyRed : nucleus
	IHC-P analysis of human colon tissue using GTX34479 CD27 antibody [LPFS2/1611].
	IHC-P analysis of human spleen tissue using GTX34479 CD27 antibody [LPFS2/1611].
	IHC-P analysis of human tonsil tissue using GTX34479 CD27 antibody [LPFS2/1611].
	IHC-P analysis of human tonsil tissue using GTX34479 CD27 antibody [LPFS2/1611].
	Analysis of Protein Array containing more than 19,000 full-length human proteins using CD27 Mouse Monoclonal Antibody (LPFS2/1611). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target if the Monoclonal Antibody has an S-score of at least 2.5. For example, if a Monoclonal Antibody binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Monoclonal Antibody to protein X is equal to 29.

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製品情報

Host	Mouse
Clonality	Monoclonal
Clone Name	LPFS2/1611
Isotype	IgG1
Application	ICC/IF, IHC-P, FACS, ELISA, Protein Array
Reactivity	Human

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APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
ICC/IF	1-2μg/ml
IHC-P	1-2μg/ml for 30 minutes at RT
FACS	1-2μg/10⁶ cells
ELISA	Assay dependent
Protein Array	Assay dependent
Note : IHC-P Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes. ELISA For ELISA coating, recommend using BSA-free format (please contact us for PBS only format).

Not tested in other applications.

Calculated MW	29 kDa. ( Note )

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PROPERTIES

Form	Liquid
Buffer	10mM PBS, 0.05% BSA, 0.05% sodium azide (Please contact us for PBS only format)
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration	0.2 mg/ml
Antigen Species	Human
Immunogen	Recombinant full-length human CD27 protein
Purification	Protein A/G purified
Conjugation	Unconjugated
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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TARGET

Synonyms	CD27,CD27 molecule,S152,S152. LPFS2,T14,TNFRSF7,Tp55
Cellular Localization	Membrane
Background	The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. This receptor transduces signals that lead to the activation of NF-kappaB and MAPK8/JNK. Adaptor proteins TRAF2 and TRAF5 have been shown to mediate the signaling process of this receptor. CD27-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor. [provided by RefSeq, Jul 2008]
Database	・ Gene ID: 939 CD27 ・ UniProt: P26842 CD27

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