抗NF-M抗体 | Anti-NF-M antibody

• 特長
• 価格
• Product Validated Data
• Product Details
• Application Note
• Bio-info

特長

• 高品質のウサギポリクローナル抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

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Product Validated Data

	Various tissue extracts (50 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with NF-M antibody (GTX133218) diluted at 1:1000.
	NF-M antibody detects NF-M protein at nucleus by immunofluorescent analysis.Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.Green: NF-M protein stained byNF-M antibody (GTX133218) diluted at 1:500.Red: beta Tubulin 3/ Tuj1, a neuron cell marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
	NF-M antibody detects NF-M protein expression by immunohistochemical analysis.Sample: Frozen-sectioned adult mouse cerebellum. Green: NF-M protein stained by NF-M antibody (GTX133218) diluted at 1:250.Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
	NF-M antibody detects NF-M protein expression by immunohistochemical analysis.Sample: Frozen-sectioned adult mouse hippocampus. Green: NF-M protein stained by NF-M antibody (GTX133218) diluted at 1:250.Red: NeuN, stained by NeuN antibody [2Q158] (GTX30773) diluted at 1:500.

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Product Details

name	NF-M antibody
Host / Clonality	Rabbit Polyclonal
Applications	ICC/IF, IHC-Fr, WB
Species reactivity	Mouse, Rat
Product Name	NF-M antibodySee all NF-M products
Predicted Target Size	96 kDa
Positive Controls	mouse brain , rat brain
Form Supplied	Liquid
Concentration	0.1mg/ml(Please refer to the vial label for the specific concentration)
Purification	Purified by antigen-affinity chromatography.
Full Name	neurofilament, medium polypeptide
Background	Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber.
Synonyms	NF M antibody , NF160 antibody , NF165 antibody , Nef3 antibody , Nefm antibody , Nfm antibody , neurofilament, medium polypeptide antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Recombinant protein encompassing a sequence within the C-terminus region of mouse NF-M. The exact sequence is proprietary.
Antigen Species	Mouse
Species Reactivity	Mouse, Rat
Predicted Cross Reactivity species	Human, Xenopus laevis, Chicken, Bovine
Predict Reactivity Note	Human (97%), Xenopus laevis (89%), Chicken (90%), Bovine (97%)
Conjugation	Unconjugated
Storage Buffer	1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Storage Instruction	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20° C or below. Avoid multiple freeze-thaw cycles.
Notes	For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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Application Note

	Suggested dilution	Reference
Immunocytochemistry/ Immunofluorescence (ICC/IF)	1:100-1:1000*
IHC (Frozen sections)	1:100-1:1000*
Western Blot (WB)	1:500-1:3000*

Not tested in other applications.
*Optimal dilutions/concentrations should be determined by the researcher.

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Bio-info

Target Bioinfo/ Predicted Isoform Cross Reactivity
Species	Gene name	Gene ID	SwissProt	OMIM	Isoform (mRNA)	Protein Accession#	MW
Mus musculus	Nefm	18040	P08553		NM_008691	NP_032717	95.93

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