抗Anti-Mouse CXCL1/GRO alpha/KC/CINC-1 MAb (Clone 48415R)抗体(Anti-Mouse CXCL1/GRO alpha/KC/CINC-1 MAb (Clone 48415R antibody)

掲載日情報:2021/01/28 現在Webページ番号:232692

Anti-Mouse CXCL1/GRO alpha/KC/CINC-1 MAb (Clone 48415R)に対する抗体(Anti-Mouse CXCL1/GRO alpha/KC/CINC-1 MAb (Clone 48415R )です。
本製品は研究用です。研究用以外には使用できません。

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[在庫・価格 :2025年04月26日 20時55分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
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Anti-Mouse CXCL1/GRO alpha/KC/CINC-1 MAb (Clone 48415R)
2~3週間 ※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。 0
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説明文
※受注発注品。
クローン:48415R
Genbank No: 14825
Protein Accession No: P12850
法規制等
保存条件 法規備考
抗原種 免疫動物 Rat クラス IgG 標識 Unlabeled
交差性 Mouse 適用 ELISA,Neutralising,Western Blot
クロナリティ Recombinant フォーマット 性状 Protein A/G Affinity Purified 吸収処理
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製品記事 M1/M2 Macrophage Activation Marker
使いっきり抗体
関連記事 R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド
Anti-Mouse CXCL1/GRO alpha/KC/CINC-1 MAb (Clone 48415R)
10日程度 ※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。 0
  • メーカーサイト
  • お問い合わせ
説明文
クローン:48415R
Genbank No: 14825
Protein Accession No: P12850
法規制等
保存条件 -20℃ 法規備考
抗原種 免疫動物 Rat クラス IgG 標識 Unlabeled
交差性 Mouse 適用 ELISA,Neutralising,Western Blot
クロナリティ Recombinant フォーマット 性状 Protein A/G Affinity Purified 吸収処理
掲載カタログ

製品記事 M1/M2 Macrophage Activation Marker
関連記事 R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド

[在庫・価格 :2025年04月26日 20時55分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。

Anti-Mouse CXCL1/GRO alpha/KC/CINC-1 MAb (Clone 48415R)

文献数: 0

説明文 ※受注発注品。
クローン:48415R
Genbank No: 14825
Protein Accession No: P12850
法規制等
保存条件 法規備考
抗原種 免疫動物 Rat
交差性 Mouse 適用 ELISA,Neutralising,Western Blot
標識 Unlabeled 性状 Protein A/G Affinity Purified
吸収処理 クラス IgG
クロナリティ Recombinant フォーマット
掲載カタログ

製品記事 M1/M2 Macrophage Activation Marker
使いっきり抗体
関連記事 R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド

Anti-Mouse CXCL1/GRO alpha/KC/CINC-1 MAb (Clone 48415R)

文献数: 0

説明文 クローン:48415R
Genbank No: 14825
Protein Accession No: P12850
法規制等
保存条件 -20℃ 法規備考
抗原種 免疫動物 Rat
交差性 Mouse 適用 ELISA,Neutralising,Western Blot
標識 Unlabeled 性状 Protein A/G Affinity Purified
吸収処理 クラス IgG
クロナリティ Recombinant フォーマット
掲載カタログ

製品記事 M1/M2 Macrophage Activation Marker
関連記事 R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド



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Product Details

Species ReactivityMouse
LabelUnconjugated
ImmunogenE. coli-derived recombinant mouse CXCL-1/GRO alpha /KC/CINC‑1Arg20-Lys96Accession # P12850
SourceRecombinant Monoclonal Rat IgG2A Clone # 48415R
PurificationProtein A or G purified from cell culture supernatant
SpecificityDetects mouse CXCL-1/GRO alpha /KC/CINC‑1 in direct ELISAs.


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Applications and Data

 Recommended
Concentration
Sample
Western Blot1 µg/mLRecombinant Mouse CXCL1/GRO alpha /KC/CINC‑1 aa 20-96 (Catalog # 453-KC)
Mouse CXCL1/KC Sandwich ImmunoassayReagent
ELISA Capture (Matched Antibody Pair)2-8 µg/mL Mouse CXCL1/GRO alpha /KC/CINC‑1 Antibody (Catalog #MAB453R )
ELISA Detection (Matched Antibody Pair)0.1-0.4 µg/mL Mouse CXCL1/GRO alpha /KC/CINC‑1 Biotinylated Antibody (Catalog #BAF453 )
ELISA Standard  Recombinant Mouse CXCL1/KC Protein (Catalog #453-KC )
NeutralizationMeasured by its ability to neutralize CXCL1/GRO alpha /KC/CINC‑1-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR2. The Neutralization Dose (ND50) is typically 0.05-0.25 µg/mL in the presence of 30 ng/mL Recombinant Mouse CXCL1/GRO alpha /KC/CINC‑1 aa 20‑96.
Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website.

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Data Examples

Neutralization CXCL1/GRO alpha/KC/CINC-1 Antibody Block/Neutralize
click image to view larger
Chemotaxis Induced by CXCL1/GRO alpha /KC/CINC-1 and Neutralization by Mouse CXCL1/GRO alpha /KC/CINC-1 Antibody.Recombinant Mouse CXCL1/GRO alpha /KC/CINC-1 aa 20-96 (Catalog # 453-KC) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CXCL1/GRO alpha /KC/CINC-1 aa 20-96 (30 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CXCL1/GRO alpha /KC/CINC-1 Monoclonal Antibody (Catalog # MAB453R). The ND50 is typically0.05-0.25 µg/mL.
ReconstitutionReconstitute at 0.5 mg/mL in sterile PBS.Reconstitution Buffer Available
ShippingThe product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CXCL1/GRO alpha/KC/CINC-1CXCL1, also known as KC and GRO alpha, is a CXC family chemokine that is expressed in fibroblasts, macrophages, and endothelial cells and interacts with CXCR2. CXCL1 is a potent neutrophil attractant and activator. It also promotes angiogenesis by inducing VEGF production by neutrophils. N-terminally truncated CXCL1 demonstrates increased potency in CXCR2 signaling. Mature mouse CXCL1 shares 96% and 64% aa sequence identity with rat and human CXCL1, respectively.
Entrez Gene IDs:2919 (Human); 14825 (Mouse); 81503 (Rat)
Alternate Names:CINC1; CINC-1; CXCL1; GRO alpha; KC; MGSA-alpha

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Related Research Areas

Alpha Granule Soluble Molecules
Chemokines and Receptors in Allergy and Asthma
Chemokines and Receptors in Angiogenesis
CXC Chemokines and Receptors
Inflammatory Mediators
Macrophage Activation Markers


Neutralization
Chemotaxis Induced by CXCL1/GRO alpha /KC/CINC-1 and Neutralization by Mouse CXCL1/GRO alpha /KC/CINC-1 Antibody.
Recombinant Mouse CXCL1/GRO alpha /KC/CINC-1 aa 20-96 (Catalog # 453-KC) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CXCL1/GRO alpha /KC/CINC-1 aa 20-96 (30 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CXCL1/GRO alpha /KC/CINC-1 Monoclonal Antibody (Catalog # MAB453R). The ND50 is typically0.05-0.25 µg/mL.


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Related Product & Information

Entrez Gene IDs2919 (Human); 14825 (Mouse); 81503 (Rat)
BackgroundCXCL1/GRO alpha/KC/CINC-1
background_contentBackground:
CXCL1/GRO alpha/KC/CINC-1
CXCL1, also known as KC and GRO alpha, is a CXC family chemokine that is expressed in fibroblasts, macrophages, and endothelial cells and interacts with CXCR2. CXCL1 is a potent neutrophil attractant and activator. It also promotes angiogenesis by inducing VEGF production by neutrophils. N-terminally truncated CXCL1 demonstrates increased potency in CXCR2 signaling. Mature mouse CXCL1 shares 96% and 64% aa sequence identity with rat and human CXCL1, respectively.


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