抗Anti-Human IL-18 MAb (Clone 925008)抗体(Anti-Human IL-18 MAb (Clone 925008 antibody)
掲載日情報：2019/10/21 現在Webページ番号：232632
Anti-Human IL-18 MAb (Clone 925008)に対する抗体(Anti-Human IL-18 MAb (Clone 925008 )です。
※ 本製品は研究用です。研究用以外には使用できません。
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価格
[在庫・価格 ：2024年06月18日 00時01分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
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[在庫・価格 ：2024年06月18日 00時01分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
Anti-Human IL-18 MAb (Clone 925008)
文献数： 0
- 商品コード：MAB2548-100
- メーカー：RSD
- 包装：100μg
- 価格：¥93,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | Protein Accession No： Q14116 | ||
|---|---|---|---|
| 法規制等 | |||
| 保存条件 | -20℃ | 法規備考 | |
| 抗原種 | 免疫動物 | Mouse | |
| 交差性 | Human | 適用 | ELISA,FCM,IC |
| 標識 | Unlabeled | 性状 | Protein A/G Affinity Purified |
| 吸収処理 | クラス | ||
| クロナリティ | Monoclonal | フォーマット | |
| 掲載カタログ |
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| 製品記事 |
M1/M2 Macrophage Activation Marker Helper T Cells Marker Antibodies |
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| 関連記事 | |||
Anti-Human IL-18 MAb (Clone 925008)
文献数： 0
- 商品コード：MAB2548-SP
- メーカー：RSD
- 包装：25μg
- 価格：¥30,000
- 在庫：無(未発注)
- 納期：2～3週間 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 |
※受注発注品。 Protein Accession No： Q14116 |
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| 法規制等 | |||
| 保存条件 | -20℃ | 法規備考 | |
| 抗原種 | 免疫動物 | Mouse | |
| 交差性 | Human | 適用 | ELISA,FCM,IC |
| 標識 | Unlabeled | 性状 | Protein A/G Affinity Purified |
| 吸収処理 | クラス | ||
| クロナリティ | Monoclonal | フォーマット | |
| 掲載カタログ |
|
||
| 製品記事 |
M1/M2 Macrophage Activation Marker Helper T Cells Marker Antibodies |
||
| 関連記事 | |||
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Product Details
| Species Reactivity | Human |
|---|---|
| Label | Unconjugated |
| Immunogen | E. coli-derived recombinant human IL-18/IL-1F4Tyr37-Asp193Accession # Q14116 |
| Source | Monoclonal Mouse IgG1 Clone # 925008 |
| Purification | Protein A or G purified from hybridoma culture supernatant |
| Specificity | Detects human IL-18/IL-1F4 in direct ELISAs. |
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Applications and Data
| Recommended Concentration | Sample | |
| ELISA | This antibody functions as an ELISA detection antibody when paired with Rabbit Anti-Human IL‑18/IL‑1F4 Monoclonal Antibody (Catalog # MAB91243). This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human Total IL-18 DuoSet ELISA Kit (Catalog # DY318-05) for convenient development of a sandwich ELISA or the Human Total IL-18/IL-1F4 Quantikine ELISA Kit (Catalog # DL180) for a complete optimized ELISA. | |
| CyTOF-ready | Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation. | |
| Immunocytochemistry | 5-25 µg/mL | See below |
| Intracellular Staining by Flow Cytometry | 0.25 µg/106 cells | See below |
| Immunocytochemistry | |
|---|---|
 | IL‑18/IL‑1F4 in Mouse Splenocytes. IL‑18/IL‑1F4 was detected in immersion fixed mouse splenocytes stimulated with Cal and PMA using Mouse Anti-Human IL‑18/IL‑1F4 Monoclonal Antibody (Catalog # MAB2548) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells. |
| Intracellular Staining by Flow Cytometry | |
 | Detection of IL‑18/IL‑1F4 in Human THP-1 cell line by Flow Cytometry. THP-1 Human acute monocytic leukemia cell line was stained with Mouse Anti-Human IL‑18/IL‑1F4 Monoclonal Antibody (Catalog # MAB2548, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by PE-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.View our protocol for Staining Intracellular Molecules. |
| ELISA | |
 | Human IL‑18/IL‑1F4 ELISA Standard Curve. Recombinant Human IL‑18/IL‑1F4 protein was serially diluted 2-fold and captured by Rabbit Anti-Human IL‑18/IL‑1F4 Monoclonal Antibody (Catalog # MAB91243) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human IL‑18/IL‑1F4 Monoclonal Antibody (Catalog # MAB2548) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994). |
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Related Product & Information
| Background | IL-18/IL-1F4 |
|---|---|
| background_content | Background: IL-18/IL-1F4 Interleukin-18 (IL-18), also known as IL-1F4 and IFN-gamma inducing factor (IGIF), is a member of the IL-1 family of cytokines and is a key molecule in the innate immune response (1). Rhesus IL-18 is synthesized as a 24 kDa proprotein that contains a 36 amino acid (aa) propeptide and a 157 aa mature region (2). Under inflammatory conditions, the propeptide is cleaved by Caspase-1 in the cytoplasm to liberate the mature nonglycosylated 18 kDa monomeric IL-18 (3, 4). Mature rhesus IL-18 shares 96% aa sequence identity with human IL-18 and 60-76% with mouse, rat, canine, feline, and porcine IL-18. IL-18 is secreted by a variety of cell types including macrophages, dendritic cells, and epithelial cells (1, 5). Circulating mature IL-18 is sequestered by soluble IL-18 binding proteins (IL-18 BP) that inhibit IL-18 bioactivity (6). IL-18 interacts with the widely expressed IL-18 R alpha which then recruits the signaling subunit IL-18 R beta (7, 8). The IL-1 family member IL-1F7 also binds to IL-18 R alpha but does not recruit IL-18 R beta or induce signaling (9). IL-1F7 binds IL-18 BP and enhances its neutralizing effect on IL-18 activity (9). IL-18 synergizes with other cytokines to activate NK, Th1, and Th17 cells and to increase the production of IFN-gamma (1, 5, 10-12). IL-18 can also promote Th2 cytokine release which reduces the effectiveness of antiviral responses (13, 14). Increased levels of active IL-18 contribute to the severity of autoimmunity and hypertension, while deficiency of IL-18 results in symptoms of metabolic syndrome (1, 5, 15, 16). In cancer, IL-18 stimulates Th1 and NK cells to target tumor cells, but it can also promote angiogenesis, metastasis, and tumor cell immune evasion (11). |
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