抗F4/80 [CI:A3-1 (recombinant version)]組換え抗体 | Anti-F4/80 [CI:A3-1 (recombinant version)] recombinant antibody
掲載日情報:2019/10/24 現在Webページ番号:216808
Absolute Antibody社の抗F4/80 [CI:A3-1 (recombinant version)]組換え抗体(リコンビナント抗体)( Anti-F4/80 [CI:A3-1 (recombinant version)] recombinant antibody)です。
※本製品は研究用です。研究用以外には使用できません。
追加しました。
価格
[在庫・価格 :2025年04月25日 20時15分現在]
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Anti-F4/80, Rat IgG2b, Fc Silent, Recombinant Monoclonal Antibody, clone CI:A3-1 (recombinant version) |
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Anti-F4/80, Rat IgG2b, Fc Silent, Recombinant Monoclonal Antibody, clone CI:A3-1 (recombinant version) |
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[在庫・価格 :2025年04月25日 20時15分現在]
Anti-F4/80, Rat IgG2b, Fc Silent, Recombinant Monoclonal Antibody, clone CI:A3-1 (recombinant version)
文献数: 0
- 商品コード:Ab00106-8.4
- メーカー:ABA
- 包装:0.2mg
- 価格:¥141,000
- 在庫:無(未発注)
- 納期:2~3週間 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等:
説明文 | 組換え体抗体。Azide Free。バッファー組成:PBS with 0.02% Proclin 300. 別名:mouse macrophage marker,EMR1 receptor,EGF-like module,Cell surface glycoprotein F4/80,EGF-like module receptor 1,Em Gpf4,TM7,Ly-71,f480,f-480,f4 80,f 480,f-4/80,f4-80 クローン:Cl:A3-1 (recombinant version) |
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保存条件 | -20℃ | 法規備考 | |
抗原種 | 免疫動物 | Rat | |
交差性 | Mouse | 適用 | FCM,IHC |
標識 | Unlabeled | 性状 | Protein A/G Affinity Purified |
吸収処理 | クラス | IgG | |
クロナリティ | Recombinant | フォーマット | |
掲載カタログ |
ニュース2019年4月15日号 p.24
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製品記事 | 抗マウスF4/80組換え抗体 | Anti-Mouse F4/80 recombinant antibody Fc Silent組換え抗体 | Fc Silent recombinant antibody 技術情報:抗体のFcドメインのタンパク質工学的改変 |
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関連記事 |
Anti-F4/80, Rat IgG2b, Fc Silent, Recombinant Monoclonal Antibody, clone CI:A3-1 (recombinant version)
文献数: 0
- 商品コード:Ab00106-8.4-BT
- メーカー:ABA
- 包装:1mg
- 価格:ご照会ください
- 在庫:無(未発注)
- 納期:2~3週間 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等:
説明文 | 組換え体抗体。Azide Free。バッファー組成:PBS only. 別名:mouse macrophage marker,EMR1 receptor,EGF-like module,Cell surface glycoprotein F4/80,EGF-like module receptor 1,Em Gpf4,TM7,Ly-71,f480,f-480,f4 80,f 480,f-4/80,f4-80 クローン:Cl:A3-1 (recombinant version) |
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法規制等 | |||
保存条件 | 法規備考 | ||
抗原種 | 免疫動物 | Rat | |
交差性 | Mouse | 適用 | FCM,IHC |
標識 | Unlabeled | 性状 | Protein A/G Affinity Purified |
吸収処理 | クラス | IgG | |
クロナリティ | Recombinant | フォーマット | |
掲載カタログ |
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製品記事 | Fc Silent組換え抗体 | Fc Silent recombinant antibody 技術情報:抗体のFcドメインのタンパク質工学的改変 |
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関連記事 |
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製品情報
Description | Recombinant monoclonal antibody to F4/80. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma CI:A3-1 (recombinant version). This antibody was created using our proprietary Fc Silent™ engineered Fc domain containing key point mutations that abrogate binding to Fc gamma receptors. |
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Species and Isotype | Rat IgG2b, Fc Silent™, kappa |
Clone Number | CI:A3-1 (recombinant version) |
UniProt Accession Number of Target Protein | Q61549 |
Alternative Name(s) of Target | EGF-like module; Cell surface glycoprotein F4/80; EGF-like module receptor 1; EMR1 receptor; Em Gpf4; TM7; Ly-71 |
Published Application(s) | FC; IHC |
Published Species Reactivity | Mouse |
Immunogen | Thioglycollate stimulated peritoneal macrophages of mouse origin. |
Specificity | This antibody recognises the mouse F4/80 antigen, a 160kD glycoprotein, expressed by murine macrophages. |
Application Notes | The anti-F4/80 antibody, clone Cl:A3-1, has been very widely used in flow cytometry, microscopy and Western blotting since its generation by Austyn and Gordon in 1980. We are the only supplier of recombinant versions of Cl:A3-1 which, whilst having the same variable domains as the original, has been shown to block hybridoma derived anti-F4/80:APC binding to in vitrogenerated bone marrow-derived (BMDMs) and ex vivosplenic murine macrophages. This was tested using both the recombinant version of the original isotype (rat IgG2b) as well as a murinised version (IgG2a, Ab00106-2.0). Further testing on BMDMs shows that our Fc Silent™ rat IgG2b is a valuable new reagent which shows distinct labelling of F4/80-positive cell populations, whilst the isotype control shows no binding, suggesting that only the antigen binding domains are involved in binding, promoting precision of labelling. The murinised versions will be valuable tools in the study of the role of the murine macrophage in vivo without the artefacts rat-derived antigen being co-administered may harbour. Particularly the murine IgG2a (Ab00106-2.0) should be an excellent candidate for macrophage depletion studies, with the Fc Silent™ (Ab00106-2.3) version being the ideal negative control. Using our recombinant production platform, any species and isotype are possible and our Support Team (support@absoluteantibody.com) will be glad to help you with finding the right variant to match your experimental needs. |
Antibody First Published in | Austyn JM, Gordon S. F4/80, a monoclonal antibody directed specifically against the mouse macrophage. Eur J Immunol. 1981 Oct;11(10):805-15. PMID:7308288 |
Note on publication | Describes the making of the antibody and its specificity for macrophages (Flow Cytometry) and proposes its potential use as a marker. |
Purification | Protein A affinity purified |
Supplied in | Bulk size: PBS only. Standard size: PBS with 0.02% Proclin 300. |
Storage Recommendation | Store at 4°C for up to 3 months. For longer term storage aliquot into small volumes and store at -20°C |
Concentration | See vial label. |
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