Ubiquitin Activating Enzyme 組換え体(リコンビナント)タンパク質 | Recombinant Human Ubiquitin Activating Enzyme
掲載日情報:2018/02/08 現在Webページ番号:216419
R&D Systems社製の高品質なUbiquitin Activating Enzymeの組換え体タンパク質(Recombinant Human Ubiquitin Activating Enzyme)です。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 :2025年04月25日 18時55分現在]
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Recombinant Human Ubiquitin Activating Enzyme (UBE1), CF |
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[在庫・価格 :2025年04月25日 18時55分現在]
Recombinant Human Ubiquitin Activating Enzyme (UBE1), CF
文献数: 0
- 商品コード:E-305-025
- メーカー:RSD
- 包装:25μg
- 価格:¥35,000
- 在庫:1個
- 納期:10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等:カルタヘナ該当品
説明文 | M.W.:118 kDa,純度:>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.,ブランド:Boston Biochem,由来動物:Human,Genbank:7317,産生:Sf 21 (baculovirus) 別名:A1S9T Genbank No: 7317 Protein Accession No: P22314 |
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法規制等 | カルタヘナ該当品 | ||
保存条件 | -80℃ | 法規備考 | |
掲載カタログ |
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製品記事 | ユビキチンのE1酵素(R&D Systems社) ユビキチン・プロテアソーム特集 |
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関連記事 |
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バルク注文に関して
※バルク包装品も承ります。お気軽にお問合せ下さい。
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CF(Carrier-Free)とは?
R&D Systems社組換え体タンパク質製品では通常ウシ血清アルブミン(BSA)をキャリアタンパク質として加えています。キャリアタンパク質を加えることで組換え体タンパク質の安定性が高まり、使用期限が長くなります。また、より濃度の低い溶液での保管も可能となります。CF製品はBSAが含まれない製品となります。一般的に細胞培養や、ELISAのスタンダードにはBSA含有製品を推奨しています。CF製品はBSAが影響してしまうアプリケーションに推奨されます。
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Product Details
- Purity
- >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain
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Related Product & Information
- Background
- Ubiquitin-activating Enzyme/UBE1
- Ubiquitin-activating Enzyme (UBE1), also known as Ubiquitin-like Modifier Activating Enzyme 1 (UBA1), is a 1058 amino acid (aa) canonical member of the Ubiquitin-activating (E1) enzyme family of proteins with a predicted molecular weight of 118 kDa. It is ubiquitously expressed and highly conserved; mouse and rat UBE1 share 95% and 96% aa sequence identity with the human UBE1 protein, respectively. UBE1 is found in the cytoplasm and nucleus, and contains a conserved active-site cysteine residue and ATP-binding site common to E1 enzymes (1-3). UBE1 is responsible for the first step in Ubiquitin-protein isopeptide bond formation (4,5). Ubiquitin is activated by UBE1 and thereafter linked to the side chain of a cysteine residue in UBE1, Cys632 in humans, yielding a Ubiquitin-UBE1 conjugate via a thioester bond (5-8). The activated Ubiquitin is then transferred to a lysine residue on the target protein via the Ubiquitin-conjugating – Ubiquitin ligase enzyme cascade. UBE1 is required for cell cycle progression and has been linked to cellular responses to DNA damage such as nucleotide excision repair (3,9,10). Mutations in UBE1 are associated with X-linked lethal infantile spinal muscular atrophy (11). UBE1 is a critical component for the initiation of in vitro ubiquitin conjugation reactions.
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Citations
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
Authors: A Murali, J Shin, H Yurugi, A Krishnan, M Akutsu, A Carpy, B Macek, K Rajalingam
Cell Death Dis, 2017;8(6):e2900.
Species: Human
Sample Type: Purified Proteins
Application: Bioassay
Authors: A Bononi, C Giorgi, S Patergnani, D Larson, K Verbruggen, M Tanji, L Pellegrini, V Signorato, F Olivetto, S Pastorino, M Nasu, A Napolitano, G Gaudino, P Morris, G Sakamoto, LK Ferris, A Danese, A Raimondi, C Tacchetti, S Kuchay, HI Pass, EB Affar, H Yang, P Pinton, M Carbone
Nature, 2017;546(7659):549-553.
Species: N/A
Sample Type: Protein
Application: Bioassay
Authors: V Glorian, J Allègre, J Berthelet, B Dumetier, PM Boutanquoi, N Droin, C Kayaci, J Cartier, S Gemble, G Marcion, D Gonzalez, R Boidot, C Garrido, O Michaud, E Solary, L Dubrez
Cell Death Dis, 2017;8(5):e2816.
Species: Human
Sample Type: Recombinant Protein
Application: Bioassay
Authors: V Glorian, J Allègre, J Berthelet, B Dumetier, PM Boutanquoi, N Droin, C Kayaci, J Cartier, S Gemble, G Marcion, D Gonzalez, R Boidot, C Garrido, O Michaud, E Solary, L Dubrez
Cell Death Dis, 2017;8(5):e2816.
Species: Bacteria - E. Coli
Sample Type: Protein
Application: Bioassay
Authors: S Schunter, R Villa, V Flynn, JB Heidelberg, AK Classen, P Beli, PB Becker
PLoS ONE, 2017;12(5):e0177408.
Species: Spodoptera frugiperda - Fall Army Worm
Sample Type: Protein
Application: Bioassay
Authors: B Zhang, M Holmlund, S Lorrain, M Norberg, L Bakó, C Fankhauser, O Nilsson
Elife, 2017;6(0):.
Species: Human
Sample Type: Recombinant Protein
Application: Bioassay
Authors: JH Kim, S Shin, J Seo, EW Lee, M Jeong, MS Lee, HJ Han, J Song
Sci Rep, 2017;7(0):40023.
Species: Human
Sample Type: Cell Lysates
Application: Ubiquitination
Authors: J Qiu, MJ Sheedlo, K Yu, Y Tan, ES Nakayasu, C Das, X Liu, ZQ Luo
Nature, 2016;533(7601):120-4.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Authors: H Zhang, H Liu, Y Chen, X Yang, P Wang, T Liu, M Deng, B Qin, C Correia, S Lee, J Kim, M Sparks, AA Nair, DL Evans, KR Kalari, P Zhang, L Wang, Z You, SH Kaufmann, Z Lou, H Pei
Nat Commun, 2016;7(0):10201.
Species: Human
Sample Type: Purified Proteins
Application: Bioassay
Oncogene, 2016;0(0):.
Application: Bioassay
Elife, 2016;5(0):.
Application: Bioassay
Nucleic Acids Res., 2016;0(0):.
Application: Bioassay
Sci Rep, 2016;6(0):36171.
Species: Human
Sample Type: Protein
Application: Bioassay
PLoS Biol., 2016;14(11):e2000733.
Application: Bioassay
J Biol Chem, 2016;0(0):.
Species: Human
Sample Type: Protein
Application: Ubiquitination
J Biol Chem, 2016;0(0):.
Application: Bioassay
Authors: Kumarkrishna Raychaudhu
J Biol Chem, 2016;0(0):.
Species: N/A
Sample Type: Protein
Application: Bioassay
Authors: Mary E Matyskiela
Nature, 2016;0(0):.
Species: N/A
Sample Type: Protein
Application: Bioassay
Authors: LA Link, BV Howley, GS Hussey, PH Howe
Mol Cancer Res, 2016;0(0):.
Species: Mouse
Sample Type: Cell Lysates
Application: Bioassay
Authors: Kim C, Yun N, Lee J, Youdim M, Ju C, Kim W, Han P, Oh Y
Cell Death Differ, 2016;23(2):333-46.
Species: N/A
Sample Type: Protein
Application: Enzyme Assay
Authors: Hsiao H, Hsu T, Liu W, Hsieh W, Chou T, Wu Y, Jiang S, Lai M
Nat Commun, 2015;6(0):6353.
Species: N/A
Sample Type: Protein
Application: Bioassay
Authors: Xue J, Chen Y, Wu Y, Wang Z, Zhou A, Zhang S, Lin K, Aldape K, Majumder S, Lu Z, Huang S
Nat Commun, 2015;6(0):6156.
Species: Human
Sample Type: Protein
Application: Bioassay
Authors: Kumar, Ravindra, Whitehurst, Christop, Pagano, Joseph S
J Virol, 2014;88(11):6411-22.
Species: N/A
Sample Type: Protein
Application: Bioassay
Authors: Lee, T-H, Park, J-M, Leem, S-H, Kang, T-H
Oncogene, 2014;33(1):19-25.
Species: N/A
Sample Type: Protein
Application: Bioassay
Authors: Rajbhandari P, Schalper K, Solodin N, Ellison-Zelski S, Ping Lu K, Rimm D, Alarid E
Oncogene, 2014;33(11):1438-47.
Species: N/A
Sample Type: Recombinant Protein
Application: Bioassay
Authors: Yue, Shen, Tang, Liu-Ya, Tang, Ying, Tang, Yi, Shen, Qiu-Hong, Ding, Jie, Chen, Yan, Zhang, Zengdi, Yu, Ting-Tin, Zhang, Ying E, Cheng, Steven Y
Elife, 2014;3(0):.
Species: N/A
Sample Type: Protein
Application: Bioassay
Authors: Rout, Manoj K, Hodge, Curtis D, Markin, Craig J, Xu, Xin, Glover, J N Mark, Xiao, Wei, Spyracopoulos, Leo
J Am Chem Soc, 2014;136(50):17446-58.
Species: N/A
Sample Type: Protein
Application: Bioassay
Authors: Kim J, Park K, Lee E, Jang W, Seo J, Shin S, Hwang K, Song J
Cell Death Differ, 2014;21(4):594-603.
Species: Human
Sample Type: Recombinant Protein
Application: EnzAct
Authors: Townley-Tilson, W H Davi, Wu, Yaxu, Ferguson, James E, Patterson, Cam
PLoS ONE, 2014;9(2):e89451.
Species: Human
Sample Type: Whole Cells
Application: Ubiquitination
Authors: Liu, Weixiao, Shang, Yonglian, Zeng, Yan, Liu, Chao, Li, Yanchang, Zhai, Linhui, Wang, Pan, Lou, Jizhong, Xu, Ping, Ye, Yihong, Li, Wei
EMBO J, 2014;33(1):46-61.
Species: N/A
Sample Type: Recombinant Protein
Application: Bioassay
Authors: Lee, Hyojin, Alpi, Arno F, Park, Mi So, Rose, Ann, Koo, Hyeon-So
PLoS ONE, 2013;8(3):e60071.
Species: Human
Sample Type: Recombinant Protein
Application: EnzAct
Authors: Hornburger M, Mayer B, Leonhardt S, Willer E, Zahler S, Beyerle A, Rajalingam K, Vollmar A, Furst R
FASEB J, 2013;28(4):1938-46.
Species: Human
Sample Type: Recombinant Protein
Application: Ubiquitination
Authors: Singer, Alexande, Schulze, Sebastia, Skarina, Tatiana, Xu, Xiaohui, Cui, Hong, Eschen-Lippold, Lennart, Egler, Monique, Srikumar, Tharan, Raught, Brian, Lee, Justin, Scheel, Dierk, Savchenko, Alexei, Bonas, Ulla
PLoS Pathog, 2013;9(1):e1003121.
Species: N/A
Sample Type: Recombinant Protein
Application: Bioassay
Authors: Bosch D, Siderovski D
J Biol Chem, 2013;288(4):2290-302.
Species: Amoeba - Entamoeba histolytica
Sample Type: Recombinant Protein
Application: Bioassay
Authors: Lonskaya I, Desforges N, Hebron M, Moussa C
PLoS ONE, 2013;8(12):e83914.
Species: N/A
Sample Type: Recombinant Protein
Application: Enzyme Assay
Authors: Varfolomeev, Eugene, Moradi, Elham, Dynek, Jasmin N, Zha, Jiping, Fedorova, Anna V, Deshayes, Kurt, Fairbrother, Wayne J, Newton, Kim, Le Couter, Jennifer, Vucic, Domagoj
Biochem J, 2012;447(3):427-36.
Species: Human
Sample Type: Protein
Application: Bioassay
Authors: Wang, Jingya, Muntean, Andrew G, Wu, Laura, Hess, Jay L
J Biol Chem, 2012;287(52):43410-6.
Species: Human
Sample Type: Recombinant Protein
Application: Ubiquination
Authors: Bell JL, Malyukova A, Holien JK
PLoS ONE, 2012;7(5):e37470.
Species: Human
Sample Type: Cell Lysates
Application: Bioassay
Authors: Pelisch F, Pozzi B, Risso G, Munoz M, Srebrow A
J Biol Chem, 2012;287(36):30789-99.
Species: N/A
Sample Type: Protein
Application: Bioassay
Authors: Stanley D, Bartholomeeusen K, Crosby D, Kim D, Kwon E, Yen L, Cartozo N, Li M, Jager S, Mason-Herr J, Hayashi F, Yokoyama S, Krogan N, Harris R, Peterlin B, Gross J
PLoS Pathog, 2012;8(12):e1003085.
Species: N/A
Sample Type: Recombinant Protein
Application: Bioassay
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