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Recombinant 組換え体(リコンビナント)タンパク質 | Wnt-3a, Human, Recombinant

掲載日情報：2018/02/08 現在Webページ番号：216337

アールアンドディー システムス /
R&D Systems, Inc.
[メーカー略称：RSD]

R&D Systems社製の高品質なRecombinantの組換え体タンパク質(Wnt-3a, Human, Recombinant)です。
※本製品は研究用です。研究用以外には使用できません。

[在庫・価格 ：2024年04月20日 20時55分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
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 納期 文献数
Wnt-3a, Human, Recombinant
 10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。 117
説明文
産生：CHO，M.W.：37.4 kDa，純度：>75%, by SDS-PAGE under reducing conditions and visualized by silver stain.，エンドトキシン濃度：＜1.0 EU/μg，由来動物：Human，Genbank：89780，エンドトキシンレベル：<1.0 EU per 1 µg of the protein by the LAL method.
別名：MGC119418
Genbank No： 89780
Protein Accession No： NP_149122
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製品記事 R&D Systems社 Wnt-3a関連製品
Wnt関連製品
抗幹細胞マーカー抗体／抗造血幹細胞マーカー抗体（R&D Systems社）
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Wnt-3a, Human, Recombinant, Carrier-free
 10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。 0
説明文
産生：CHO，M.W.：37.4 kDa，純度：>75%, by SDS-PAGE under reducing conditions and visualized by silver stain.，エンドトキシン濃度：＜1.0 EU/μg，由来動物：Human，Genbank：89780，エンドトキシンレベル：<1.0 EU per 1 µg of the protein by the LAL method.
別名：MGC119418
Genbank No： 89780
Protein Accession No： NP_149122
別包装品 別包装品あり
法規制等
保存条件 -20℃ 法規備考
掲載カタログ

製品記事 R&D Systems社 Wnt-3a関連製品
Wnt関連製品
抗幹細胞マーカー抗体／抗造血幹細胞マーカー抗体（R&D Systems社）
関連記事

[在庫・価格 ：2024年04月20日 20時55分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。

Wnt-3a, Human, Recombinant

文献数： 117

説明文 産生：CHO，M.W.：37.4 kDa，純度：>75%, by SDS-PAGE under reducing conditions and visualized by silver stain.，エンドトキシン濃度：＜1.0 EU/μg，由来動物：Human，Genbank：89780，エンドトキシンレベル：<1.0 EU per 1 µg of the protein by the LAL method.
別名：MGC119418
Genbank No： 89780
Protein Accession No： NP_149122
別包装品 別包装品あり
法規制等
保存条件 -20℃ 法規備考
掲載カタログ

製品記事 R&D Systems社 Wnt-3a関連製品
Wnt関連製品
抗幹細胞マーカー抗体／抗造血幹細胞マーカー抗体（R&D Systems社）
関連記事

Wnt-3a, Human, Recombinant, Carrier-free

文献数： 0

説明文 産生：CHO，M.W.：37.4 kDa，純度：>75%, by SDS-PAGE under reducing conditions and visualized by silver stain.，エンドトキシン濃度：＜1.0 EU/μg，由来動物：Human，Genbank：89780，エンドトキシンレベル：<1.0 EU per 1 µg of the protein by the LAL method.
別名：MGC119418
Genbank No： 89780
Protein Accession No： NP_149122
別包装品 別包装品あり
法規制等
保存条件 -20℃ 法規備考
掲載カタログ

製品記事 R&D Systems社 Wnt-3a関連製品
Wnt関連製品
抗幹細胞マーカー抗体／抗造血幹細胞マーカー抗体（R&D Systems社）
関連記事

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CF(Carrier-Free)とは？

R&D Systems社組換え体タンパク質製品では通常ウシ血清アルブミン(BSA)をキャリアタンパク質として加えています。キャリアタンパク質を加えることで組換え体タンパク質の安定性が高まり、使用期限が長くなります。また、より濃度の低い溶液での保管も可能となります。CF製品はBSAが含まれない製品となります。一般的に細胞培養や、ELISAのスタンダードにはBSA含有製品を推奨しています。CF製品はBSAが影響してしまうアプリケーションに推奨されます。

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Product Details

Source
Chinese Hamster Ovary cell line, CHO-derivedSer19-Lys352
Accession #
NP_149122
Purity
>75%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Predicted Molecular Mass
37.4 kDa
SDS-PAGE
40 kDa, reducing conditions
Activity
Measured by its ability to induce alkaline phosphatase production by MC3T3‑E1 mouse preosteoblast cells. The ED50 for this effect is 5-25 ng/mL. Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The ED50 for this effect is Optimal concentrations should be determined by each laboratory for each application.

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Related Product & Information

Entrez Gene IDs
89780 (Human); 22416 (Mouse)
Background
Wnt-3a
Wnt-3a is one of 19 vertebrate members of the Wingless-type MMTV integration site (Wnt) family of highly conserved cysteine-rich secreted glycoproteins important for normal developmental processes (1). Wnts bind to the cell surface Frizzled family receptors in conjunction with low-density lipoprotein receptor-related protein family receptors (LRP5 or 6) resulting in the stabilization of intracellular beta -catenin levels (2). As intracellular beta -catenin levels rise, beta -catenin binds to TCF/LEF transcription factors leading to expression of Wnt target genes (3). Endo-IWR 1 (Catalog # 3532, # PSM1324) is a cell-permeant small molecule inhibitor of Axin turnover that suppresses Wnt signal transduction by stabilizing the beta -catenin destruction complex (4). Wnt-3a is a 44 kDa secreted hydrophobic glycoprotein containing a conserved pattern of 24 cysteine residues (5). Wnt-3a has two N-linked glycosylation sites (Asn 87, Asn 298), and Ser 209 is modified with palmitoleic acid (6). Glycosylation and acylation are essential for efficient Wnt secretion and biological activity, respectively (6, 7). Human Wnt-3a shares 96% amino acid (aa) identity with mouse mouse, bovine and canine Wnt-3a, and 89%, 86% and 84% aa identity with chicken, Xenopus and zebrafish Wnt-3a, respectively. It also shares 87% aa identity with Wnt3. During embryonic development, Wnt-3a is necessary for proper development of the hippocampus, anterior-posterior patterning, somite development, and tailbud formation (9-12). Wnt-3a also promotes self-renewal of hematopoietic stem cells, neural stem cells, and embryonic stem cells (13, 14).

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Citations

R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
Directed Differentiation of Human Induced Pluripotent Stem Cells into Fallopian Tube Epithelium
Authors: N Yucer, M Holzapfel, T Jenkins Vo, L Lenaeus, L Ornelas, A Laury, D Sareen, R Barrett, BY Karlan, CN Svendsen
Sci Rep, 2017;7(1):10741.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Wnt/?-catenin signaling suppresses expressions of Scx, Mkx, and Tnmd in tendon-derived cells
Authors: Y Kishimoto, B Ohkawara, T Sakai, M Ito, A Masuda, N Ishiguro, C Shukunami, D Docheva, K Ohno
PLoS ONE, 2017;12(7):e0182051.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Up-regulated NRIP2 in colorectal cancer initiating cells modulates the Wnt pathway by targeting ROR?
Authors: Z Wen, T Pan, S Yang, J Liu, H Tao, Y Zhao, D Xu, W Shao, J Wu, X Liu, Y Wang, J Mao, Y Zhu
Mol. Cancer, 2017;16(1):20.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
LPS-induced inflammatory response is suppressed by Wnt inhibitors, Dickkopf-1 and LGK974
Authors: J Jang, Y Jung, Y Kim, EH Jho, Y Yoon
Sci Rep, 2017;7(0):41612.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Patient-specific hepatocyte-like cells derived from induced pluripotent stem cells model pazopanib-mediated hepatotoxicity
Authors: Y Choudhury, YC Toh, J Xing, Y Qu, J Poh, L Huan, HS Tan, R Kanesvaran, H Yu, MH Tan
Sci Rep, 2017;7(0):41238.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Aristaless Related Homeobox (ARX) Interacts with ?-Catenin, BCL9, and P300 to Regulate Canonical Wnt Signaling
Authors: IT Cho, Y Lim, JA Golden, G Cho
PLoS ONE, 2017;12(1):e0170282.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Physico-electrochemical Characterization of Pluripotent Stem Cells during Self-Renewal or Differentiation by a Multi-modal Monitoring System
Authors: K Low, LY Wong, M Maldonado, C Manjunath, CB Horner, M Perez, NV Myung, J Nam
Stem Cell Reports, 2017;0(0):.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Gelatin device for the delivery of growth factors involved in endochondral ossification
Authors: LA Ahrens, D Vonwil, J Christense, VP Shastri
PLoS ONE, 2017;12(4):e0175095.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Dishevelled stability is positively regulated by PKC?-mediated phosphorylation induced by Wnt agonists
Authors: DM Velázquez, MC Castañeda-, M Robles-Flo
Cell. Signal., 2017;35(0):107-117.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Efficient Derivation of Functional Human Airway Epithelium from Pluripotent Stem Cells via Temporal Regulation of Wnt Signaling
Authors: KB McCauley, F Hawkins, M Serra, DC Thomas, A Jacob, DN Kotton
Cell Stem Cell, 2017;0(0):.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
AKAP2 is upregulated in ovarian cancer, and promotes growth and migration of cancer cells
Authors: X Li, C Wang, G Zhang, M Liang, B Zhang
Mol Med Rep, 2017;0(0):.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Generation of Human Liver Chimeric Mice with Hepatocytes from Familial Hypercholesterolemia Induced Pluripotent Stem Cells
Authors: J Yang, Y Wang, T Zhou, LY Wong, XY Tian, X Hong, WH Lai, KW Au, R Wei, Y Liu, LH Cheng, G Liang, Z Huang, W Fan, P Zhao, X Wang, DP Ibañez, Z Luo, Y Li, X Zhong, S Chen, D Wang, L Li, L Lai, B Qin, X Bao, AP Hutchins, CW Siu, Y Huang, MA Esteban, HF Tse
Stem Cell Reports, 2017;0(0):.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Loss of Usp9x disrupts cell adhesion, and components of the Wnt and Notch signaling pathways in neural progenitors
Authors: S Premarathn, M Murtaza, N Matigian, LA Jolly, SA Wood
Sci Rep, 2017;7(1):8109.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Wnt/?-catenin signaling contributes to tumor malignancy and is targetable in gastrointestinal stromal tumor
Authors: S Zeng, AM Seifert, JQ Zhang, MJ Cavnar, TS Kim, VP Balachandr, JA Santamaria, NA Cohen, MJ Beckman, B Medina, F Rossi, MH Crawley, JK Loo, JH Maltbaek, P Besmer, CR Antonescu, RP DeMatteo
Mol. Cancer Ther., 2017;0(0):.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Surrogate Wnt agonists that phenocopy canonical Wnt and ?-catenin signalling
Authors: CY Janda, LT Dang, C You, J Chang, W de Lau, ZA Zhong, KS Yan, O Marecic, D Siepe, X Li, JD Moody, BO Williams, H Clevers, J Piehler, D Baker, CJ Kuo, KC Garcia
Nature, 2017;0(0):.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Generation of Mouse and Human Organoid-Forming Intestinal Progenitor Cells by Direct Lineage Reprogramming
Authors: S Miura, A Suzuki
Cell Stem Cell, 2017;0(0):.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
DOT1L safeguards cartilage homeostasis and protects against osteoarthritis
Authors: S Monteagudo, FMF Cornelis, C Aznar-Lope, P Yibmantasi, LA Guns, P Carmeliet, F Cailotto, RJ Lories
Nat Commun, 2017;8(0):15889.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Wnt3a Induces the Expression of Acetylcholinesterase during Osteoblast Differentiation via the Runx2 Transcription Factor
Authors: ML Xu, CWC Bi, EYL Liu, TTX Dong, KWK Tsim
J. Biol. Chem., 2017;0(0):.
Species: Rat
Sample Type: Whole Cells
Application: Bioassay
Multilineage communication regulates human liver bud development from pluripotency
Authors: JG Camp, K Sekine, T Gerber, H Loeffler-W, H Binder, M Gac, S Kanton, J Kageyama, G Damm, D Seehofer, L Belicova, M Bickle, R Barsacchi, R Okuda, E Yoshizawa, M Kimura, H Ayabe, H Taniguchi, T Takebe, B Treutlein
Nature, 2017;0(0):.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Beta-catenin inhibitor BC2059 is efficacious as monotherapy or in combination with proteasome inhibitor bortezomib in multiple myeloma
Authors: I Savvidou, T Khong, A Cuddihy, C McLean, S Horrigan, A Spencer
Mol. Cancer Ther., 2017;0(0):.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Ubiquitin carboxyl-terminal esterase L1 (UCHL1) is associated with stem-like cancer cell functions in pediatric high-grade glioma
Authors: PC Sanchez-Di, JC Chang, ES Moses, T Dao, Y Chen, JY Hung
PLoS ONE, 2017;12(5):e0176879.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Effects of hepatitis C virus core protein and nonstructural protein 4B on the Wnt/?-catenin pathway
Authors: XH Jiang, YT Xie, YP Cai, J Ren, T Ma
BMC Microbiol., 2017;17(1):124.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Accreditation of Biosafe Clinical-Grade Human Embryonic Stem Cells According to Chinese Regulations
Authors: Q Gu, J Wang, L Wang, ZX Liu, WW Zhu, YQ Tan, WF Han, J Wu, CJ Feng, JH Fang, L Liu, L Wang, W Li, XY Zhao, BY Hu, J Hao, Q Zhou
Stem Cell Reports, 2017;0(0):.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
TNIK inhibition abrogates colorectal cancer stemness
Nat Commun, 2016;7(0):12586.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Activation of endothelial ?-catenin signaling induces heart failure
Sci Rep, 2016;6(0):25009.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
PGE2 is a direct and robust mediator of anion/fluid secretion by human intestinal epithelial cells
Sci Rep, 2016;6(0):36795.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Growth hormone is permissive for neoplastic colon growth
Proc Natl Acad Sci USA, 2016;0(0):.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
ROCK inhibitor primes human induced pluripotent stem cells to selectively differentiate towards mesendodermal lineage via epithelial-mesenchymal transition-like modulation
Stem Cell Res, 2016;17(2):222-227.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Wnt signaling promotes breast cancer by blocking ITCH-mediated degradation of the YAP/TAZ transcriptional coactivator WBP2
Cancer Res, 2016;0(0):.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Tumor-Free Transplantation of Patient-Derived Induced Pluripotent Stem Cell Progeny for Customized Islet Regeneration
Stem Cells Transl Med, 2016;5(5):694-702.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Anthelmintic niclosamide disrupts the interplay of p65 and FOXM1/?-catenin and eradicates leukemia stem cells in chronic myelogenous leukemia
Clin Cancer Res, 2016;0(0):.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Quantifying ?-catenin subcellular dynamics and cyclin D1 mRNA transcription during Wnt signaling in single living cells
Elife, 2016;5(0):.
Species: Mouse
Sample Type: Whole Cells
Application: Bioassay
Modeling Smith-Lemli-Opitz syndrome with induced pluripotent stem cells reveals a causal role for Wnt/?-catenin defects in neuronal cholesterol synthesis phenotypes
Nat. Med., 2016;22(4):388-96.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Aberrantly expressed LGR4 empowers Wnt signaling in multiple myeloma by hijacking osteoblast-derived R-spondins
Proc. Natl. Acad. Sci. U.S.A, 2016;0(0):.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
The AKAP GSKIP regulates beta-catenin through both its interactions with PKA and GSK3beta
J Biol Chem, 2016;0(0):.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Downregulation of the Wnt inhibitor CXXC5 predicts a better prognosis in acute myeloid leukemia.
Authors: Kuhnl A, Valk P, Sanders M, Ivey A, Hills R, Mills K, Gale R, Kaiser M, Dillon R, Joannides M, Gilkes A, Haferlach T, Schnittger S, Duprez E, Linch D, Delwel R, Lowenberg B, Baldus C, Solomon E, Burnett A, Grimwade D
Blood, 2015;125(19):2985-94.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Hepcidin regulation in prostate and its disruption in prostate cancer.
Authors: Tesfay L, Clausen K, Kim J, Hegde P, Wang X, Miller L, Deng Z, Blanchette N, Arvedson T, Miranti C, Babitt J, Lin H, Peehl D, Torti F, Torti S
Cancer Res, 2015;75(11):2254-63.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Resveratrol ameliorates the maturation process of beta-cell-like cells obtained from an optimized differentiation protocol of human embryonic stem cells.
Authors: Pezzolla D, Lopez-Beas J, Lachaud C, Dominguez-Rodriguez A, Smani T, Hmadcha A, Soria B
PLoS ONE, 2015;10(3):e0119904.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Transcription factor binding dynamics during human ES cell differentiation.
Authors: Tsankov, Alexande, Gu, Hongcang, Akopian, Veronika, Ziller, Michael, Donaghey, Julie, Amit, Ido, Gnirke, Andreas, Meissner, Alexande
Nature, 2015;518(7539):344-9.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Tumour suppressor TRIM33 targets nuclear beta-catenin degradation.
Authors: Xue J, Chen Y, Wu Y, Wang Z, Zhou A, Zhang S, Lin K, Aldape K, Majumder S, Lu Z, Huang S
Nat Commun, 2015;6(0):6156.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
microRNA-103/107 Family Regulates Multiple Epithelial Stem Cell Characteristics.
Authors: Peng H, Park J, Katsnelson J, Kaplan N, Yang W, Getsios S, Lavker R
Stem Cells, 2015;33(5):1642-56.
A protein kinase A (PKA)/beta-catenin pathway sustains the BMP2/DLX3-induced osteogenic differentiation in dental follicle cells (DFCs).
Authors: Viale-Bouroncle S, Klingelhoffer C, Ettl T, Reichert T, Morsczeck C
Cell Signal, 2015;27(3):598-605.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Canonical Wnt pathway inhibitor ICG-001 induces cytotoxicity of multiple myeloma cells in Wnt-independent manner.
Authors: Grigson E, Ozerova M, Pisklakova A, Liu H, Sullivan D, Nefedova Y
PLoS ONE, 2015;10(1):e0117693.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Activin-A and Bmp4 levels modulate cell type specification during CHIR-induced cardiomyogenesis.
Authors: Kim M, Horst A, Blinka S, Stamm K, Mahnke D, Schuman J, Gundry R, Tomita-Mitchell A, Lough J
PLoS ONE, 2015;10(2):e0118670.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Efficient generation of functional CFTR-expressing airway epithelial cells from human pluripotent stem cells.
Authors: Wong, Amy P, Chin, Stephani, Xia, Sunny, Garner, Jodi, Bear, Christin, Rossant, Janet
Nat Protoc, 2015;10(3):363-81.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Epsin is required for Dishevelled stability and Wnt signalling activation in colon cancer development.
Authors: Chang B, Tessneer K, McManus J, Liu X, Hahn S, Pasula S, Wu H, Song H, Chen Y, Cai X, Dong Y, Brophy M, Rahman R, Ma J, Xia L, Chen H
Nat Commun, 2015;6(0):6380.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
A Regulatory Network Involving beta-Catenin, e-Cadherin, PI3k/Akt, and Slug Balances Self-Renewal and Differentiation of Human Pluripotent Stem Cells In Response to Wnt Signaling.
Authors: Huang T, Li L, Moalim-Nour L, Jia D, Bai J, Yao Z, Bennett S, Figeys D, Wang L
Stem Cells, 2015;33(5):1419-33.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Robust derivation of epicardium and its differentiated smooth muscle cell progeny from human pluripotent stem cells.
Authors: Iyer D, Gambardella L, Bernard W, Serrano F, Mascetti V, Pedersen R, Talasila A, Sinha S
Development, 2015;142(8):1528-41.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Focal adhesion kinase and Wnt signaling regulate human ductal carcinoma in situ stem cell activity and response to radiotherapy.
Authors: Williams K, Bundred N, Landberg G, Clarke R, Farnie G
Stem Cells, 2015;33(2):327-41.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Transcriptome analysis of wnt3a-treated triple-negative breast cancer cells.
Authors: Maubant S, Tesson B, Maire V, Ye M, Rigaill G, Gentien D, Cruzalegui F, Tucker G, Roman-Roman S, Dubois T
PLoS ONE, 2015;10(4):e0122333.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Endogenously produced Indian Hedgehog regulates TGFbeta-driven chondrogenesis of human bone marrow stromal/stem cells.
Authors: Handorf A, Chamberlain C, Li W
Stem Cells Dev, 2015;24(8):995-1007.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Characterization and propagation of tumor initiating cells derived from colorectal liver metastases: trials, tribulations and a cautionary note.
Authors: James M, Howells L, Karmokar A, Higgins J, Greaves P, Cai H, Dennison A, Metcalfe M, Garcea G, Lloyd D, Berry D, Steward W, Brown K
PLoS ONE, 2015;10(2):e0117776.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Intestinal Commitment and Maturation of Human Pluripotent Stem Cells Is Independent of Exogenous FGF4 and R-spondin1.
Authors: Tamminen K, Balboa D, Toivonen S, Pakarinen M, Wiener Z, Alitalo K, Otonkoski T
PLoS ONE, 2015;10(7):e0134551.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
The in vitro generation of lung and airway progenitor cells from human pluripotent stem cells.
Authors: Huang S, Green M, de Carvalho A, Mumau M, Chen Y, D'Souza S, Snoeck H
Nat Protoc, 2015;10(3):413-25.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Novel cancer chemotherapy hits by molecular topology: dual Akt and Beta-catenin inhibitors.
Authors: Zanni R, Galvez-Llompart M, Morell C, Rodriguez-Henche N, Diaz-Laviada I, Recio-Iglesias M, Garcia-Domenech R, Galvez J
PLoS ONE, 2015;10(4):e0124244.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Rapid and efficient differentiation of human pluripotent stem cells into intermediate mesoderm that forms tubules expressing kidney proximal tubular markers.
Authors: Lam A, Freedman B, Morizane R, Lerou P, Valerius M, Bonventre J
J Am Soc Nephrol, 2014;25(6):1211-25.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Modulation of stemness in a human normal intestinal epithelial crypt cell line by activation of the WNT signaling pathway.
Authors: Guezguez A, Pare F, Benoit Y, Basora N, Beaulieu J
Exp Cell Res, 2014;322(2):355-64.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Nodal.Gdf1 heterodimers with bound prodomains enable serum-independent nodal signaling and endoderm differentiation.
Authors: Fuerer C, Nostro M, Constam D
J Biol Chem, 2014;289(25):17854-71.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
CD44v6 is a marker of constitutive and reprogrammed cancer stem cells driving colon cancer metastasis.
Authors: Todaro M, Gaggianesi M, Catalano V, Benfante A, Iovino F, Biffoni M, Apuzzo T, Sperduti I, Volpe S, Cocorullo G, Gulotta G, Dieli F, De Maria R, Stassi G
Cell Stem Cell, 2014;14(3):342-56.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Whole-exome sequencing reveals LRP5 mutations and canonical Wnt signaling associated with hepatic cystogenesis.
Authors: Cnossen W, te Morsche R, Hoischen A, Gilissen C, Chrispijn M, Venselaar H, Mehdi S, Bergmann C, Veltman J, Drenth J
Proc Natl Acad Sci U S A, 2014;111(14):5343-8.
sRNA-seq analysis of human embryonic stem cells and definitive endoderm reveals differentially expressed microRNAs and novel IsomiRs with distinct targets.
Authors: Hinton A, Hunter S, Afrikanova I, Jones G, Lopez A, Fogel G, Hayek A, King C
Stem Cells, 2014;32(9):2360-72.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Type I collagen as an extracellular matrix for the in vitro growth of human small intestinal epithelium.
Authors: Jabaji Z, Brinkley G, Khalil H, Sears C, Lei N, Lewis M, Stelzner M, Martin M, Dunn J
PLoS ONE, 2014;9(9):e107814.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
RSPO2-LGR5 signaling has tumour-suppressive activity in colorectal cancer.
Authors: Wu C, Qiu S, Lu L, Zou J, Li W, Wang O, Zhao H, Wang H, Tang J, Chen L, Xu T, Sun Z, Liao W, Luo G, Lu X
Nat Commun, 2014;5(0):3149.
Sphingosine-1-phosphate promotes expansion of cancer stem cells via S1PR3 by a ligand-independent Notch activation.
Authors: Hirata, Naoya, Yamada, Shigeru, Shoda, Takuji, Kurihara, Masaaki, Sekino, Yuko, Kanda, Yasunari
Nat Commun, 2014;5(0):4806.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Interleukin-1beta-induced Wnt5a enhances human corneal endothelial cell migration through regulation of Cdc42 and RhoA.
Authors: Lee J, Heur M
Mol Cell Biol, 2014;34(18):3535-45.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Wnts enhance neurotrophin-induced neuronal differentiation in adult bone-marrow-derived mesenchymal stem cells via canonical and noncanonical signaling pathways.
Authors: Tsai H, Deng W, Lai W, Chiu W, Yang C, Tsai Y, Hwang S, Renshaw P
PLoS ONE, 2014;9(8):e104937.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Influence of in vitro and in vivo oxygen modulation on beta cell differentiation from human embryonic stem cells.
Authors: Cechin S, Alvarez-Cubela S, Giraldo J, Molano R, Villate S, Ricordi C, Pileggi A, Inverardi L, Fraker C, Dominguez-Bendala J
Stem Cells Transl Med, 2014;3(3):277-89.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Differentiation of human pluripotent stem cells into nephron progenitor cells in a serum and feeder free system.
Authors: Kang, Minyong, Han, Yong-Mah
PLoS ONE, 2014;9(4):e94888.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
WNT5A induces release of exosomes containing pro-angiogenic and immunosuppressive factors from malignant melanoma cells.
Authors: Ekstrom E, Bergenfelz C, von Bulow V, Serifler F, Carlemalm E, Jonsson G, Andersson T, Leandersson K
Mol Cancer, 2014;13(0):88.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
WNT3A promotes myogenesis of human embryonic stem cells and enhances in vivo engraftment.
Authors: Hwang Y, Suk S, Shih Y, Seo T, Du B, Xie Y, Li Z, Varghese S
Sci Rep, 2014;4(0):5916.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Canonical Wnt signaling skews TGF-beta signaling in chondrocytes towards signaling via ALK1 and Smad 1/5/8.
Authors: van den Bosch M, Blom A, van Lent P, van Beuningen H, Blaney Davidson E, van der Kraan P, van den Berg W
Cell Signal, 2014;26(5):951-8.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
A synthetic peptide-acrylate surface for production of insulin-producing cells from human embryonic stem cells.
Authors: Lin P, Hung S, Yang Y, Liao L, Hsieh Y, Yen H, Lu H, Lee M, Chu I, Hwang S
Stem Cells Dev, 2014;23(4):372-9.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
RSPO-LGR4 functions via IQGAP1 to potentiate Wnt signaling.
Authors: Carmon K, Gong X, Yi J, Thomas A, Liu Q
Proc Natl Acad Sci U S A, 2014;111(13):E1221-9.
Species: Human
Sample Type: Whole Cells
Application: Bioassay

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