Recombinant 組換え体(リコンビナント)タンパク質 | Galectin-9, Human, Recombinant
掲載日情報:2018/02/08 現在Webページ番号:216312
R&D Systems社製の高品質なRecombinantの組換え体タンパク質(Galectin-9, Human, Recombinant)です。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 :2024年04月20日 00時00分現在]
詳細 | 商品名 |
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Galectin-9, Human, Recombinant, Carrier-free |
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[在庫・価格 :2024年04月20日 00時00分現在]
Galectin-9, Human, Recombinant, Carrier-free
文献数: 5
- 商品コード:2045-GA-050
- メーカー:RSD
- 包装:50μg
- 価格:¥90,000
- 在庫:1個
- 納期:10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等:
説明文 |
純度:>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.,由来動物:Human,M.W.:35.8 kDa,Genbank:3965,エンドトキシンレベル:<1.0 EU per 1 µg of the protein by the LAL method.,産生:E. coli 別名:ecalectin Genbank No: 3965 Protein Accession No: BAA31542 |
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保存条件 | -20℃ | 法規備考 | |
掲載カタログ |
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製品記事 |
Recombinant Human Galectin-9 R&D Systems社のGalectin-9関連製品 |
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関連記事 |
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バルク注文に関して
※バルク包装品も承ります。お気軽にお問合せ下さい。
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CF(Carrier-Free)とは?
R&D Systems社組換え体タンパク質製品では通常ウシ血清アルブミン(BSA)をキャリアタンパク質として加えています。キャリアタンパク質を加えることで組換え体タンパク質の安定性が高まり、使用期限が長くなります。また、より濃度の低い溶液での保管も可能となります。CF製品はBSAが含まれない製品となります。一般的に細胞培養や、ELISAのスタンダードにはBSA含有製品を推奨しています。CF製品はBSAが影響してしまうアプリケーションに推奨されます。
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Product Details
- Source
- E. coli-derivedAla2-Thr323
- Accession #
- BAA31542
- Purity
- >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
- Predicted Molecular Mass
- 35.8 kDa
- SDS-PAGE
- 34 kDa, reducing conditions
- Activity
- Measured by its ability to induce apoptosis of Jurkat human acute T cell leukemia cells. Lu, L.H. et al. (2007) J. Biochem. 141:157. The ED50 for this effect is 1-5 μg/mL. Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human Galectin-9 at 500 ng/mL can bindRecombinantHuman TIM-3 Fc Chimera (Catalog # 2365-TM)with an apparent Kd et al. (2000) J. Cell Sci. 113:2385. The ED50 for this effect is 2.5‑12.5 µg/mL.
- SDS-PAGE
- 1 μg/lane of Recombinant Human Galectin-9 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 34 kDa.
- Bioactivity
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Related Product & Information
- Entrez Gene IDs
- 3965 (Human); 16859 (Mouse)
- Background
- Galectin-9
- Galectins comprise a family of multifunctional carbohydrate-binding proteins with specificity for N‑acetyl-lactosamine-containing glycoproteins. At least 14 mammalian Galectins share structural similarities in their carbohydrate recognition domains (CRD), forming three groups: prototype (one CRD), tandem-repeat (two CRDs), and chimeric (one CRD, unique N‑terminus) (1, 2). Full length Galectin-9 is a widely expressed 39 kDa tandem-repeat Galectin that contains two CRDs connected by a linker region (3). Progressive deletion within the linker region generates a 36 kDa isoform, also known as Ecalectin or UAT, as well as a 35 kDa isoform (4). This recombinant protein corresponds to the Ecalectin isoform of human Galectin-9 and shares 70% and 73% aa sequence identity with the corresponding regions of mouse and rat Galectin-9, respectively. Galectin-9 exhibits a wide range of activities. All three isoforms function as eosinophil chemoattractants (5, 6). This activity is destroyed by thrombin-mediated cleavage within the linker region of the long isoform, although the Ecalectin isoform is resistant to thrombin (7). Galectin-9 binds to carbohydrate moieties of IgE, thereby preventing immune complex formation, mast cell degranulation, and asthmatic and cutaneous anaphylaxis reactions (8). Independent of its lectin properties, Galectin-9 induces the maturation of dendritic cells which promote Th1 polarization (9). Galectin-9 induces cellular apoptosis in part by direct binding to TIM-3 (10, 11). Its interaction with TIM-3 inhibits Th1 cell and CD8+ cytotoxic T cell responses and also promotes regulatory T cell differentiation and activity (11, 12). Galectin-9 suppresses tumor cell metastasis by interfering with the associations between hyaluronic acid and CD44 and between VCAM-1 and Integrin alpha 4 beta 1 (13). The Ecalectin isoform (UAT; urate transporter) can also be expressed as an integral membrane protein and mediate the cellular efflux of urate (14).
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Citations
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
Authors: Leskela S, Serrano A, de la Fuente H, Rodriguez-Munoz A, Ramos-Levi A, Sampedro-Nunez M, Sanchez-Madrid F, Gonzalez-Amaro R, Marazuela M
PLoS ONE, 2015;10(4):e0123938.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Authors: Ungerer C, Quade-Lyssy P, Radeke H, Henschler R, Konigs C, Kohl U, Seifried E, Schuttrumpf J
Stem Cells Dev, 2014;23(7):755-66.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Authors: Vaitaitis GM, Wagner DH,
PLoS ONE, 2012;7(6):e38708.
Species: Mouse
Sample Type: Whole Cells
Application: Bioassay
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