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Flt-3 Ligand Protein 組換え体(リコンビナント)タンパク質 | Recombinant Human Flt-3 Ligand Protein

掲載日情報：2018/02/07 現在Webページ番号：215999

アールアンドディー システムス /
R&D Systems, Inc.
[メーカー略称：RSD]

R&D Systems社製の高品質なFlt-3 Ligand Proteinの組換え体タンパク質(Recombinant Human Flt-3 Ligand Protein)です。
※本製品は研究用です。研究用以外には使用できません。

[在庫・価格 ：2024年05月25日 08時35分現在]

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Recombinant Human Flt-3 Ligand Protein
 10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。 0
説明文
純度：>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.，由来動物：Human，M.W.：17.5 kDa，Genbank：2323，エンドトキシンレベル：<0.10 EU per 1 µg of the protein by the LAL method.，産生：Sf 21 (baculovirus)
別名：FL
Genbank No： 2323
Protein Accession No： AAA17999.1
法規制等
保存条件 -20℃ 法規備考
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製品記事 抗幹細胞マーカー抗体／抗造血幹細胞マーカー抗体（R&D Systems社）
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Recombinant Human Flt-3 Ligand Protein, CF
 10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。 0
説明文
純度：>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.，由来動物：Human，M.W.：17.5 kDa，Genbank：2323，エンドトキシンレベル：<0.10 EU per 1 µg of the protein by the LAL method.，産生：Sf 21 (baculovirus)
別名：FL
Genbank No： 2323
Protein Accession No： AAA17999.1
法規制等
保存条件 -20℃ 法規備考
掲載カタログ

製品記事 抗幹細胞マーカー抗体／抗造血幹細胞マーカー抗体（R&D Systems社）
関連記事

[在庫・価格 ：2024年05月25日 08時35分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。

Recombinant Human Flt-3 Ligand Protein

文献数： 0

説明文 純度：>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.，由来動物：Human，M.W.：17.5 kDa，Genbank：2323，エンドトキシンレベル：<0.10 EU per 1 µg of the protein by the LAL method.，産生：Sf 21 (baculovirus)
別名：FL
Genbank No： 2323
Protein Accession No： AAA17999.1
法規制等
保存条件 -20℃ 法規備考
掲載カタログ

製品記事 抗幹細胞マーカー抗体／抗造血幹細胞マーカー抗体（R&D Systems社）
関連記事

Recombinant Human Flt-3 Ligand Protein, CF

文献数： 0

説明文 純度：>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.，由来動物：Human，M.W.：17.5 kDa，Genbank：2323，エンドトキシンレベル：<0.10 EU per 1 µg of the protein by the LAL method.，産生：Sf 21 (baculovirus)
別名：FL
Genbank No： 2323
Protein Accession No： AAA17999.1
法規制等
保存条件 -20℃ 法規備考
掲載カタログ

製品記事 抗幹細胞マーカー抗体／抗造血幹細胞マーカー抗体（R&D Systems社）
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CF(Carrier-Free)とは？

R&D Systems社組換え体タンパク質製品では通常ウシ血清アルブミン(BSA)をキャリアタンパク質として加えています。キャリアタンパク質を加えることで組換え体タンパク質の安定性が高まり、使用期限が長くなります。また、より濃度の低い溶液での保管も可能となります。CF製品はBSAが含まれない製品となります。一般的に細胞培養や、ELISAのスタンダードにはBSA含有製品を推奨しています。CF製品はBSAが影響してしまうアプリケーションに推奨されます。

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Product Details

Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derivedThr27-Pro185
Accession #
AAA17999.1
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Predicted Molecular Mass
17.5 kDa
SDS-PAGE
17-30 kDa, reducing conditions
Activity
Measured in a cell proliferation assay using BaF3 mouse pro‑B cells transfected with mouse Flt-3. The ED50 for this effect is 0.2-1 ng/mL.The specific activity of Recombinant Human Flt-3 Ligand is approximately 1.2 x 103 U/μg, which is calibrated against recombinant humanFlt-3 Ligand WHO Standard (NIBSC code: 96/532).

Bioactivity
Recombinant Human Flt-3 Ligand (Catalog # 308-FK) stimulates cell proliferation in the BaF3 mouse pro-B cell line transfected with mouse Flt-3. The ED50 for this effect is 0.2-1 ng/mL.
SDS-PAGE
1 µg/lane of Recombinant Human Flt-3 Ligand was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing major bands at 22‑26 kDa. The multiple bands are due to variable glycosylation of the protein.

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Related Product & Information

Entrez Gene IDs
2323 (Human); 14256 (Mouse); 493796 (Feline)
Background
Flt-3 Ligand
Flt‑3 Ligand, also known as FL, is an alpha -helical cytokine that promotes the differentiation of multiple hematopoietic cell lineages (1-3). Mature human Flt‑3 Ligand consists of a 158 amino acid (aa) extracellular domain (ECD) with a cytokine-like domain and a juxtamembrane tether region, a 21 aa transmembrane segment, and a 30 aa cytoplasmic tail (4-7). Within the ECD, human Flt‑3 Ligand shares 71% and 65% aa sequence identity with mouse and rat Flt‑3 Ligand, respectively. Human and mouse Flt‑3 Ligand show cross-species activity (4-6). Flt‑3 Ligand is expressed as a noncovalently-linked dimer by T cells and bone marrow and thymic fibroblasts (1, 8). Each 36 kDa chain carries approximately 12 kDa of N- and O-linked carbohydrates (8). Alternate splicing and proteolytic cleavage of the transmembrane form can generate a soluble 30 kDa fragment that includes the cytokine domain (4, 8). Alternate splicing of human Flt‑3 Ligand also generates membrane-associated isoforms that contain either a truncated cytoplasmic tail or an 85 aa substitution following the cytokine domain (4, 5, 8). Both transmembrane and soluble Flt‑3 Ligand signal through the tyrosine kinase receptor Flt-3/Flk-2 (3, 4, 6, 7). Flt‑3 Ligand induces the expansion of monocytes and immature dendritic cells as well as early B cell lineage differentiation (2, 9). It synergizes with IL-3, GM-CSF, and SCF to promote the mobilization and myeloid differentiation of hematopoietic stem cells (4-6). It cooperates with IL-2, -6, -7, and -15 to induce NK cell development and with IL-3, -7, and -11 to induce terminal B cell maturation (1, 10). Animal studies also showFlt‑3 Ligand to reduce the severity of experimentally induced allergic inflammation (11).

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Citations

R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
Let-7 microRNA-dependent control of leukotriene signaling regulates the transition of hematopoietic niche in mice
Authors: X Jiang, JS Hawkins, J Lee, CO Lizama, FL Bos, JP Zape, P Ghatpande, Y Peng, J Louie, G Lagna, AC Zovein, A Hata
Nat Commun, 2017;8(1):128.
Species: Mouse
Sample Type: Whole Cells
Application: Bioassay
Stage-Specific Human Induced Pluripotent Stem Cells Map the Progression of Myeloid Transformation to Transplantable Leukemia
Authors: AG Kotini, CJ Chang, A Chow, H Yuan, TC Ho, T Wang, S Vora, A Solovyov, C Husser, M Olszewska, J Teruya-Fel, D Perumal, VM Klimek, A Spyridonid, RK Rampal, L Silverman, EP Reddy, E Papaemmanu, S Parekh, BD Greenbaum, CS Leslie, MG Kharas, EP Papapetrou
Cell Stem Cell, 2017;0(0):.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
A novel approach for the identification of efficient combination therapies in primary human acute myeloid leukemia specimens
Authors: I Baccelli, J Krosl, G Boucher, I Boivin, VP Lavallée, J Hébert, S Lemieux, A Marinier, G Sauvageau
Blood Cancer J, 2017;7(2):e529.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Pentraxin-2 suppresses c-Jun/AP-1 signaling to inhibit progressive fibrotic disease
JCI Insight, 2016;1(20):e87446.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Induced Pluripotent Stem Cell-Derived Natural Killer Cells for Treatment of Ovarian Cancer.
Authors: Hermanson D, Bendzick L, Pribyl L, McCullar V, Vogel R, Miller J, Geller M, Kaufman D
Stem Cells, 2016;34(1):93-101.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Reversible lineage-specific priming of human embryonic stem cells can be exploited to optimize the yield of differentiated cells.
Authors: Lee J, Graham M, Collins T, Lee J, Hong S, McNicol A, Shapovalova Z, Bhatia M
Stem Cells, 2015;33(4):1142-52.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
SCL, LMO1 and Notch1 reprogram thymocytes into self-renewing cells.
Authors: Gerby B, Tremblay C, Tremblay M, Rojas-Sutterlin S, Herblot S, Hebert J, Sauvageau G, Lemieux S, Lecuyer E, Veiga D, Hoang T
PLoS Genet, 2015;10(12):e1004768.
Species: Mouse
Sample Type: Whole Cells
Application: Bioassay
Functional Characterization of D9, a Novel Deazaneplanocin A (DZNep) Analog, in Targeting Acute Myeloid Leukemia (AML).
Authors: Jiang X, Lim C, Li Z, Lee P, Yatim S, Guan P, Li J, Zhou J, Pan J, Chng W, Chai C, Yu Q
PLoS ONE, 2015;10(4):e0122983.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Small-molecule inhibitor of glycogen synthase kinase 3beta 6-Bromoindirubin-3-oxime inhibits hematopoietic regeneration in stem cell recipient mice.
Authors: Shen S, Xu N, Klamer G, Ko K, Khoo M, Ma D, Moore J, O'Brien T, Dolnikov A
Stem Cells Dev, 2015;24(6):724-36.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Regulated apoptosis of genetically modified hematopoietic stem and progenitor cells via an inducible caspase-9 suicide gene in rhesus macaques.
Authors: Barese C, Felizardo T, Sellers S, Keyvanfar K, Di Stasi A, Metzger M, Krouse A, Donahue R, Spencer D, Dunbar C
Stem Cells, 2015;33(1):91-100.
Species: Primate - Macaca mulatta (Rhesus Macaque)
Sample Type: Whole Cells
Application: Bioassay
Prospectively Isolated Human Bone Marrow Cell-Derived MSCs Support Primitive Human CD34-Negative Hematopoietic Stem Cells.
Authors: Matsuoka Y, Nakatsuka R, Sumide K, Kawamura H, Takahashi M, Fujioka T, Uemura Y, Asano H, Sasaki Y, Inoue M, Ogawa H, Takahashi T, Hino M, Sonoda Y
Stem Cells, 2015;33(5):1554-65.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Clonal tracking of rhesus macaque hematopoiesis highlights a distinct lineage origin for natural killer cells.
Authors: Wu C, Li B, Lu R, Koelle S, Yang Y, Jares A, Krouse A, Metzger M, Liang F, Lore K, Wu C, Donahue R, Chen I, Weissman I, Dunbar C
Cell Stem Cell, 2014;14(4):486-99.
Repression of BIM mediates survival signaling by MYC and AKT in high-risk T-cell acute lymphoblastic leukemia.
Authors: Reynolds, C, Roderick, J E, LaBelle, J L, Bird, G, Mathieu, R, Bodaar, K, Colon, D, Pyati, U, Stevenson, K E, Qi, J, Harris, M, Silverman, L B, Sallan, S E, Bradner, J E, Neuberg, D S, Look, A T, Walensky, L D, Kelliher, M A, Gutierrez, A
Leukemia, 2014;28(9):1819-27.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Blood stem cell fate regulation by Delta-1-mediated rewiring of IL-6 paracrine signaling.
Authors: Csaszar, Elizabet, Wang, Weijia, Usenko, Tatiana, Qiao, Wenlian, Delaney, Colleen, Bernstein, Irwin D, Zandstra, Peter W
Blood, 2014;123(5):650-8.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Patient-specific naturally gene-reverted induced pluripotent stem cells in recessive dystrophic epidermolysis bullosa.
Authors: Tolar J, McGrath J, Xia L, Riddle M, Lees C, Eide C, Keene D, Liu L, Osborn M, Lund T, Blazar B, Wagner J
J Invest Dermatol, 2014;134(5):1246-54.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
MicroRNA screen of human embryonic stem cell differentiation reveals miR-105 as an enhancer of megakaryopoiesis from adult CD34+ cells.
Authors: Kamat V, Paluru P, Myint M, French D, Gadue P, Diamond S
Stem Cells, 2014;32(5):1337-46.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
A lack of secretory leukocyte protease inhibitor (SLPI) causes defects in granulocytic differentiation.
Authors: Klimenkova O, Ellerbeck W, Klimiankou M, Unalan M, Kandabarau S, Gigina A, Hussein K, Zeidler C, Welte K, Skokowa J
Blood, 2014;123(8):1239-49.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Characterization of CD56+ dendritic-like cells: a normal counterpart of blastic plasmacytoid dendritic cell neoplasm?
Authors: Osaki Y, Yokohama A, Saito A, Tahara K, Yanagisawa K, Ogawa Y, Ishizaki T, Mitsui T, Koiso H, Takizawa M, Uchiumi H, Saitoh T, Handa H, Murakami H, Tsukamoto N, Nojima Y
PLoS ONE, 2013;8(11):e81722.
Species: Human
Sample Type: Whole Cells
Application: cell culture
Epigenetic regulation of NANOG by miR-302 cluster-MBD2 completes induced pluripotent stem cell reprogramming.
Authors: Lee M, Prasain N, Chae H, Kim Y, Mantel C, Yoder M, Broxmeyer H
Stem Cells, 2013;31(4):666-81.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Neurokinin-1 receptor signalling impacts bone marrow repopulation efficiency.
Authors: Berger A, Frelin C, Shah D, Benveniste P, Herrington R, Gerard N, Zuniga-Pflucker J, Iscove N, Paige C
PLoS ONE, 2013;8(3):U630.
Species: Mouse
Sample Type: Whole Cells
Application: cell culture
Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors.
Authors: Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J
Nature, 2012;487(7408):505-9.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
A novel serum-free monolayer culture for orderly hematopoietic differentiation of human pluripotent cells via mesodermal progenitors.
Authors: Niwa A, Heike T, Umeda K
PLoS ONE, 2011;6(7):e22261.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Induced pluripotent stem cells offer new approach to therapy in thalassemia and sickle cell anemia and option in prenatal diagnosis in genetic diseases.
Authors: Ye L, Chang JC, Lin C, Sun X, Yu J, Kan YW
Proc. Natl. Acad. Sci. U.S.A., 2009;106(24):9826-30.
Isolation and characterization of CD146+ multipotent mesenchymal stromal cells.
Authors: Sorrentino A, Ferracin M, Castelli G, Biffoni M, Tomaselli G, Baiocchi M, Fatica A, Negrini M, Peschle C, Valtieri M
Exp. Hematol., 2008;36(8):1035-46.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Refreezing of cord blood hematopoietic stem cells for allogenic transplantation: in vitro and in vivo validation of a clinical phase I/II protocol in European and Italian Good Manufacturing Practice conditions.
Authors: Gunetti M, Ferrero I, Rustichelli D, Berger M, Gammaitoni L, Timeus F, Piacibello W, Aglietta M, Fagioli F
Exp. Hematol., 2008;36(2):235-43.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Thrombopoietin enhances generation of CD34+ cells from human embryonic stem cells.
Authors: Srivastava AS, Nedelcu E, Esmaeli-Azad B, Mishra R, Carrier E
Stem Cells, 2007;25(6):1456-61.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Tal1/Scl gene transduction using a lentiviral vector stimulates highly efficient hematopoietic cell differentiation from common marmoset (Callithrix jacchus) embryonic stem cells.
Authors: Kurita R, Sasaki E, Yokoo T, Hiroyama T, Takasugi K, Imoto H, Izawa K, Dong Y, Hashiguchi T, Soda Y, Maeda T, Suehiro Y, Tanioka Y, Nakazaki Y, Tani K
Stem Cells, 2006;24(9):2014-22.
Species: Primate - Callitrix jacchus (Common Marmoset)
Sample Type: Whole Cells
Application: Bioassay
Effects of busulfan dose escalation on engraftment of infant rhesus monkey hematopoietic stem cells after gene marking by a lentiviral vector.
Authors: Kahl CA, Tarantal AF, Lee CI, Jimenez DF, Choi C, Pepper K, Petersen D, Fletcher MD, Leapley AC, Fisher J, Burns TS, Ultsch MN, Dorey FJ, Kohn DB
Exp. Hematol., 2006;34(3):369-81.
Species: Primate - Macaca mulatta (Rhesus Macaque)
Sample Type: Whole Cells
Application: Bioassay
Long-lasting in vitro hematopoiesis derived from primate embryonic stem cells.
Authors: Hiroyama T, Miharada K, Aoki N, Fujioka T, Sudo K, Danjo I, Nagasawa T, Nakamura Y
Exp. Hematol., 2006;34(6):760-9.
Species: Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
Sample Type: Whole Cells
Application: Bioassay
The reduction of in vitro radiation-induced Fas-related apoptosis in CD34+ progenitor cells by SCF, FLT-3 ligand, TPO, and IL-3 in combination resulted in CD34+ cell proliferation and differentiation.
Authors: Drouet M, 49153, Mathieu J, Grenier N, Multon E, Sotto JJ, Herodin F
Circulating NOD1 Activators and Hematopoietic NOD1 Contribute to Metabolic Inflammation and Insulin Resistance, 1999;17(5):273-85.
Species: Primate - Baboon
Sample Type: Whole Cells
Application: Bioassay

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