抗Mouse IL-33 Alexa Fluor 405抗体(Anti-Mouse IL-33 Alexa Fluor 405 antibody)

掲載日情報:2018/11/26 現在Webページ番号:212799

Mouse IL-33 Alexa Fluor 405に対する抗体(Anti-Mouse IL-33 Alexa Fluor 405 )です。
本製品は研究用です。研究用以外には使用できません。

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Anti-Mouse IL-33 Alexa Fluor 405 Antibody (Clone 396118)
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説明文
別名:C9orf26
クローン:396118
Genbank No: 90865
Protein Accession No: Q8BVZ5
法規制等
保存条件 法規備考
抗原種 免疫動物 Rat クラス IgG 標識 Alexa Fluor 405
交差性 Mouse 適用 FCM
クロナリティ Monoclonal フォーマット 性状 Protein A/G Affinity Purified 吸収処理
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製品記事 Helper T Cells Marker Antibodies
関連記事

[在庫・価格 :2024年06月17日 20時55分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。

Anti-Mouse IL-33 Alexa Fluor 405 Antibody (Clone 396118)

文献数: 0

説明文 別名:C9orf26
クローン:396118
Genbank No: 90865
Protein Accession No: Q8BVZ5
法規制等
保存条件 法規備考
抗原種 免疫動物 Rat
交差性 Mouse 適用 FCM
標識 Alexa Fluor 405 性状 Protein A/G Affinity Purified
吸収処理 クラス IgG
クロナリティ Monoclonal フォーマット
掲載カタログ

製品記事 Helper T Cells Marker Antibodies
関連記事



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Product Details

Species ReactivityMouse
LabelAlexa Fluor 405
ImmunogenE. coli-derived recombinant mouse IL-33Ser109-Ile266Accession # Q8BVZ5
SourceMonoclonal Rat IgG2A Clone # 396118
PurificationProtein A or G purified from hybridoma culture supernatant
SpecificityDetects mouse IL-33 in direct ELISAs.


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Applications and Data

 Recommended
Concentration
Sample
Intracellular Staining by Flow Cytometry0.25-1 µg/106 cellsSee below


Intracellular Staining by Flow Cytometry
Detection of IL‑33 in bEnd.3 Mouse Cell Line by Flow Cytometry.
bEnd.3 mouse endothelioma cell line was stained with Rat Anti-Mouse IL‑33 Alexa Fluor® 405‑conjugated Monoclonal Antibody (Catalog # IC3626V, filled histogram) or isotype control antibody (Catalog # IC006V, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.


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Related Product & Information

BackgroundIL-33
background_contentBackground:
IL-33
IL-33, also known as NF-HEV and DVS 27, is a 30 kDa proinflammatory protein that may also regulate gene transcription (1-3). DVS 27 was identifed as a gene that is upregulated in vasospastic cerebral arteries (1). NF-HEV was described as a nuclear factor that is preferentially expressed in the endothelial cells of high endothelial venules relative to endothelial cells from other tissues (2). IL-33 was identified based on sequence and structural homology with IL-1 family cytokines (3). DVS 27, NF-HEV, and IL-33 share 100% amino acid sequence identity. IL-33 is constitutively expressed in smooth muscle and airway epithelia. It is upregulated in arterial smooth muscle, dermal fibroblasts, and keratinocytes following IL-1 alpha or IL-1 beta stimulation (1, 3). Similar to IL-1, IL-33 can be cleaved in vitro by caspase-1, generating an N-terminal fragment that is slightly shorter than the C-terminal fragment (3, 4). The N-terminal portion of full length IL-33 contains a predicted bipartite nuclear localization sequence and a homeodomain-like helix-turn-helix DNA binding domain. By immunofluorescence, full length IL-33 localizes to the nucleus in HUVECs and transfectants (2). The C-terminal fragment, corresponding to mature IL-33, binds and triggers signaling through mast cell IL-1 R4/ST2L, a longtime orphan receptor involved in the augmentation of Th2 cell responses (3, 5-7). A ternary signaling complex is formed by the subsequent association of IL-33 and ST2L with IL-1R AcP (8). Stimulation of Th2 polarized lymphocytes with mature IL-33 in vitro induces IL-5 and IL-13 secretion (3). In vivo administration of mature IL-33 promotes increased production of IL-5, IL-13, IgE, and IgA, as well as splenomegaly and inflammatory infiltration of mucosal tissues (3). Full length and mature mouse IL-33 share approximately 55% and 90% amino acid (aa) sequence identity with human and rat IL-33, respectively. Mouse IL-33 shares less than 25% aa sequence identity with other IL-1 family proteins.


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