抗Polyglutamate chain (polyE), pAb (IN105)抗体 | Anti-Polyglutamate chain (polyE), pAb (IN105) antibody | Adipogen
掲載日情報：2017/10/30 現在Webページ番号：205377
Adipogen社の抗Polyglutamate chain (polyE), pAb (IN105)抗体(Anti-Polyglutamate chain (polyE), pAb (IN105) antibody)です。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2024年05月05日 00時00分現在]
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Anti-Polyglutamate Chain (polyE), Rabbit-Poly |
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[在庫・価格 ：2024年05月05日 00時00分現在]
Anti-Polyglutamate Chain (polyE), Rabbit-Poly
文献数： 0
- 商品コード：AG-25B-0030-C050
- メーカー：KOM
- 包装：50μg
- 価格：¥99,000
- 在庫：無(未発注)
- 納期：2週間程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | |||
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| 法規制等 | |||
| 保存条件 | 4℃,凍結禁止 | 法規備考 | |
| 抗原種 | 免疫動物 | Rabbit | |
| 交差性 | All | 適用 | IC,IHC,Western Blot |
| 標識 | Unlabeled | 性状 | |
| 吸収処理 | クラス | ||
| クロナリティ | Polyclonal | フォーマット | |
| 掲載カタログ |
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| 製品記事 |
チューブリン翻訳後修飾（PTM）関連抗体 細胞周期・微小管研究用製品特集 |
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製品説明
Microtubules are key elements of the eukaryotic cytoskeleton that dynamically assemble from heterodimers of α- and β-tubulin. Two different mechanisms can generate microtubule diversity: the expression of different α- and β-tubulin genes, referred to as tubulin isotypes, and the generation of posttranslational modifications (PTMs) on α- and β-tubulin. Tubulin PTMs include the well-known acetylation or phosphorylation, and others that have so far mostly been found on tubulin, detyrosination/tyrosination, polyglutamylation and polyglycylation. These PTMs might have evolved to specifically regulate tubulin and microtubule functions. Polyglutamylation is a PTM that occurs when secondary glutamate side chains are formed on γ-carboxyl groups of glutamate residues in a protein. Enzymes catalyzing polyglutamylation belong to the TTL-like (TTLL; Tubulin tyrosine ligase-like) family of glutamylases. Deglutamylases, the enzymes that reverse polyglutamylation, were identified within a novel family of CCPs (cytosolic carboxypeptidase). Subtle differences in polyglutamylation can be seen on diverse microtubules in different cell types. The functions of these modifications remain to be studied. However, its wide distribution strengthens the idea that it could be involved in fine-tuning a range of microtubule functions.PolyE labels centrioles as they mature, such that two foci are present throughout the cell cycle.
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製品情報
| Source/Host | Rabbit |
|---|---|
| Immunogen/Antigen | Polyglutamate peptide. |
| Application |
Immunocytochemistry: (1:5'000) Western Blot: (1:2'000) |
| Crossreactivity | All |
| Specificity | Recognizes C-terminally located linear glutamate chains of 4 and more glutamate residues. |
| Purity Detail | Epitope-affinity purified. |
| Concentration | 0.5mg/ml |
| Formulation | Liquid. In PBS containing 0.02% sodium azide. |
| Product Type | Polyclonal Antibody |
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Western blot analysis of protein polyglutamylation by anti-Polyglutamate chain (polyE), pAb (IN105) (Prod. No. AG-25B-0030).Method: HEK-293T cells are grown in standard culture conditions, transfected with plasmids expressing the tubulin glutamylases TTLL4 and TTLL6 (J. van Dijk, et al.; Mol. Cell 2007) and are lysed in SDS sample buffer and run on a 10% SDS-PAGE. Brain tubulin was prepared following standard procedures (M. Castoldi & A.V. Popov; Protein Expr. Purif. 2003). Different C-terminally modified variants of GST-telokin were produced in bacteria as described before (K. Rogowski, et al.; Cell 2010). The proteins are transferred to a nitrocellulose membrane and detected by standard immunoblot protocol using the anti-polyE, pAb (IN105) (1:2'000) in TBS containing 0.1% Tween-20 for washing steps and 2.5% fat free milk for antibody incubation. The same samples were probed with the anti-Polyglutamylation Modification, mAb (GT335) (Prod. No. AG-20B-0020) for comparison. In the normal HEK-293T cell extracts, no tubulin detection is observed, even on high exposure. Expression of TTLL4 glutamylase does not lead to detection with anti-polyE, pAb (IN105) while glutamylation is observed with GT335. This indicates that TTLL4 generates short glutamate chains on tubulin. Expression of TTLL6 generates long glutamate chains (J. van Dijk, et al.; Mol. Cell 2007), which are detected by anti-polyE, pAb (IN105). Brain tubulin is a heterogenous mixture of different glutamylation states of tubulin (B. Edde, et al.; Science 1990) and therefore strongly detected by anti-polyE, pAb (IN105) and mAb GT335. Anti-polyE, pAb (IN105) also detects gene-encoded linear C-terminal glutamate chains. To illustrate the specificity of anti-polyE, pAb (IN105), recombinant GST-telokin proteins are included in the western blot analysis. Anti-polyE, pAb (IN105) specifically detects glutamate-chains of four and more glutamates. In contrast, GT335 cannot detect such chains (K. Rogowski, et al.; Cell 2010).Picture courtesy of Dr. Sudarshan Gadadhar & Dr. Carsten Janke, Curie Institute, Paris |
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