抗Caspase-8 (mouse), mAb (3B10)抗体 | Anti-Caspase-8 (mouse), mAb (3B10) antibody | Adipogen
掲載日情報：2017/10/30 現在Webページ番号：205198
Adipogen社の抗Caspase-8 (mouse), mAb (3B10)抗体(Anti-Caspase-8 (mouse), mAb (3B10) antibody)です。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2024年05月18日 00時00分現在]
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[在庫・価格 ：2024年05月18日 00時00分現在]
Anti-Caspase-8 (mouse), mAb (3B10)
文献数： 0
- 商品コード：AG-20T-0138-C100
- メーカー：KOM
- 包装：100μg
- 価格：¥73,000
- 在庫：無(未発注)
- 納期：2週間程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 |
別名：Apoptotic Cysteine Protease，Apoptotic Protease Mch-5，CAP4，FADD-homologous ICE/ced-3-like Protease クローン：3B10 Protein Accession No： O89110 |
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| 法規制等 | |||
| 保存条件 | -20℃ | 法規備考 | |
| 抗原種 | Mouse | 免疫動物 | Rat |
| 交差性 | Mouse | 適用 | ELISA,FCM,IC,IHC,Western Blot |
| 標識 | Unlabeled | 性状 | |
| 吸収処理 | クラス | IgG | |
| クロナリティ | Monoclonal | フォーマット | |
| 掲載カタログ |
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| 製品記事 |
インフラマソーム（Inflammasome）研究用抗体 PANoptosome Antibody Markers for PANoptosis (Pyroptosis, Apoptosis, and Necroptosis) PANoptosome Human/Mouse Antibodies Starter Set |
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製品説明
Procaspase-8 belongs to the family of caspases. Binding of FasL to Fas leads to formation of a receptor complex at the cellular membrane, which was named DISC. The DISC consists of oligomerized receptors, the DD-containing adaptor molecule FADD, procaspase-8, procaspase-10 and c-FLIP. The DISC structure provides a platform for the oligomerization of procaspase-8 that allows two procaspase-8 homodimers to be in the close proximity leading to the initial activation of procaspase-8. At the first cleavage step, the N-terminal p43/p41 and the C-terminal p30 cleavage products are generated. Importantly, these cleavage products already possess catalytic activity. At the second cleavage step, p43/p41 and p30 are processed to p10 and p20, respectively, which leads to the generation of the active caspase-8 heterotetramer (p20/p10)2.
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製品情報
| Clone | 3B10 |
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| Isotype | Rat IgG1κ |
| Immunogen/Antigen | Recombinant mouse caspase-8 (p20 subunit). |
| Application |
ELISA: (recombinant protein) Flow Cytometry: (overexpressed in cell lines) Immunocytochemistry: (excellent, see literature reference 2) Immunohistochemistry Western Blot: (1-2μg/ml) Optimal conditions must be determined individually for each application. |
| Crossreactivity | Mouse |
| Specificity | Recognizes the p20 subunit of mouse caspase-8. Detects bands of ~55kDa (full-length caspase-8) and ~18kDa (apoptosis-induced cleavage fragment) by Western blot. Does not cross-react with human caspase-8. |
| Purity | ≥95% (SDS-PAGE) |
| Purity Detail | Protein G-affinity purified (sepharose column). |
| Endotoxin Content | |
| Concentration | 1mg/ml |
| Formulation | Liquid. In PBS containing 0.02% sodium azide. |
| Product Type | Monoclonal Antibody |
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画像
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Flow cytometry data in permeabilized cells of overexpressed FLAG-epitope-tagged pro-caspase-8 (active-site-mutant) in 293T cells using anti-caspase-8 mAbs (3B10) (Prod. No. AG-20T-0138), anti-FLAG or control. As a positive control staining with a MAb to FLAG was performed. As a negative control an isotype control was employed. |
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Western blot using anti-Caspase-8 (mouse), mAb (3B10) (Prod. No. AG-20T-0138) detecting endogenous caspase-8 in various mouse cell line, but not in human cell lines. |
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Western blot using anti-Caspase-8 (mouse), mAb (3B10) (Prod. No. AG-20T-0138) detecting the cleaved active p20 subunit of mouse caspase-8 in addition to the caspase-8 precursor, upon an apoptotic stimulus e.g. by cross-linked rhsFasL. |
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Western blot using anti-Caspase-8 (mouse), mAb (3B10) (Prod. No. AG-20T-0138) detecting endogenous caspase-8 in MEFs from WT mice, but not in MEFs from caspase-8-/- mice. Several smaller bands detected in the caspase-8-/- MEFs, correspond to truncated forms of caspase-8 made in the caspase-8-/- mice since only exons 1 and 2 of mouse caspase-8 were deleted in these knock-out mice and not the region encoding the p20 subunit. Note: Extra bands marked by * are only seen in lysates from caspase-8-/- MEFs and not in lysates from any WT cell lines or mouse WT tissue. |
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