抗Polyglutamylation Modification, mAb (GT335)抗体 | Anti-Polyglutamylation Modification, mAb (GT335) antibody | Adipogen
掲載日情報：2017/10/30 現在Webページ番号：205099
Adipogen社の抗Polyglutamylation Modification, mAb (GT335)抗体(Anti-Polyglutamylation Modification, mAb (GT335) antibody)です。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2025年04月26日 00時00分現在]
| 詳細 | 商品名 |
|
文献数 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
|
Anti-Polyglutamylation Modification, mAb (GT335) |
|
7 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
|
|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
[在庫・価格 ：2025年04月26日 00時00分現在]
Anti-Polyglutamylation Modification, mAb (GT335)
文献数： 7
- 商品コード：AG-20B-0020-C100
- メーカー：KOM
- 包装：100μg
- 価格：¥104,000
- 在庫：無(未発注)
- 納期：2週間程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | Recognizes most forms of polyglutamylated tubulin( - and -tubulin), independent of the length of theglutamate side chains. No specificity to particular tubulinisoforms nor to tubulin from particular speciesare observed. Detects also other (poly)glutamylatedproteins. Since no consensus modification site isknown for protein (poly)glutamylation, the detectionis not sequence-specific. However, an acidic environmentof the modification site is required.。純度：>95% (SDS-PAGE) 別名：Polyglutamylated Tubulin，Glutamylated Tubulin，Postranslational Protein Glutamylation クローン：GT335 |
||
|---|---|---|---|
| 法規制等 | |||
| 保存条件 | -20℃ | 法規備考 | |
| 抗原種 | 免疫動物 | Mouse | |
| 交差性 | All | 適用 | Electron Microscopy,IC,IHC,IP,Western Blot |
| 標識 | Unlabeled | 性状 | |
| 吸収処理 | クラス | IgG | |
| クロナリティ | Monoclonal | フォーマット | |
| 掲載カタログ |
|
||
| 製品記事 | Anti-Polyglutamylation Modification Antibody チューブリン翻訳後修飾（PTM）関連抗体 細胞周期・微小管研究用製品特集 |
||
| 関連記事 | |||
追加しました。
製品説明
Polyglutamylation is a post-translational modification in which glutamate side chains of variable lengths are formed on the modified protein. It is evolutionarily conserved and the most prominent substrate is tubulin, the microtubule (MT) building block. Polyglutamylation has been proposed to be involved in the functional adaptation of MTs, as it occurs within the carboxy-terminal tubulin tails that participate directly in the binding of many structural and motor MT-associated proteins. The recent identification of new substrates of polyglutamylation indicates that this post-translational modification could be a potential regulator of diverse cellular processes and be involved in cell cycle and cell proliferation.
追加しました。
製品情報
| Clone | GT335 |
|---|---|
| Isotype | Mouse IgG1κ |
| Source/Host | Purified from concentrated hybridoma tissue culture supernatant. |
| Immunogen/Antigen | Octapeptide EGEGE*EEG, modified by the addition of two glutamyl units onto the fifth E (indicated by an asterisk). |
| Application |
Electron Microscopy (see Lit. 3) Immunocytochemistry: (1:2000) Immunoprecipitation Western Blot: (1:4000) Optimal conditions must be determined individually for each application. |
| Crossreactivity | All |
| Specificity | Recognizes the posttranslational modification (poly)glutamylation on proteins. Reacts with polyglutamylated α- and β-tubulin. |
| Purity | ≥95% (SDS-PAGE) |
| Purity Detail | Protein G-affinity purified. |
| Concentration | 1mg/ml |
| Formulation | Liquid. In PBS containing 10% glycerol and 0.02% sodium azide. |
| Other Product Data | Recognizes most forms of polyglutamylated tubulin (α- and β-tubulin), independent of the length of the glutamate side chains. No specificity to particular tubulin isoforms nor to tubulin from particular species are observed. Detects also other (poly)glutamylated proteins. Since no consensus modification site is known for protein (poly)glutamylation, the detection is not sequence-specific. However, an acidic environment of the modification site is required. The use of the antibody at too high concentrations obscures its specificity in immunofluorescence. |
| Product Type | Monoclonal Antibody |
追加しました。
画像
 |
Immunofluorescence analysis of tubulin glutamylation with anti-Polyglutamylation Modification, mAb (GT335) (Prod. No. AG-20B-0020).HeLa cells grown in standard culture conditions are transfected with GFP-fusion expression plasmids for the polyglutamylases TTLL4 and TTLL6. Glutamylated microtubules are detected with anti-Polyglutamylation Modification, mAb (GT335). Only in the cells expressing glutamylases (red arrowheads), strong microtubule labelling is detected, whereas in non-transfected cells (white arrowheads), only very weak labelling is seen. Note that the level of endogenous microtubule glutamylation can vary between different cell lines.Picture courtesy of Dr. Carsten Janke, Curie Institute, Paris. |
 |
Western blot analysis of protein glutamylation with MAb to polyglutamylation modification (GT335) (Prod. No. AG-20B-0020).Method: HeLa cells grown in standard culture conditions are lysed in SDS sample buffer and run on 10% SDS-PAGE. The proteins are transferred to nitrocellulose membrane and detected by standard Western blot protocol, using the MAb to polyglutamylation modification (GT335) (1:1’000) in TBS containing 0.1% Tween 20 for washing steps and 5% fat free milk for antibody incubation.In HeLa cells, only two non-tubulin proteins are detected (Regnard et al., 2000; van Dijk et al., 2008), whereas afterthe expression of either TTLL4 or TTLL6 polyglutamylases (van Dijk et al., 2007), tubulin and additional proteins aredetected. Brain tubulin is highly polyglutamylated and therefore strongly detected with the MAb to polyglutamylation modification (GT335).Picture courtesy of Dr Carsten Janke, Curie Institute, Paris |
追加しました。
製品情報は掲載時点のものですが、価格表内の価格については随時最新のものに更新されます。お問い合わせいただくタイミングにより製品情報・価格などは変更されている場合があります。
表示価格に、消費税等は含まれていません。一部価格が予告なく変更される場合がありますので、あらかじめご了承下さい。