Human, Mouse and Rat Phospho-SHC (Tyr427) and Total SHC ELISA Kit

掲載日情報:2017/06/21 現在Webページ番号:204123

RayBiotech社のHuman, Mouse and Rat Phospho-SHC (Tyr427) and Total SHC ELISA Kitです。
本製品は研究用です。研究用以外には使用できません。

RayBiotech社ELISA kit

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[在庫・価格 :2024年04月30日 00時00分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
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Human, Mouse and Rat Phospho-SHC (Tyr427) and Total SHC ELISA Kit (96-Well Strip Microplate)
10日程度 ※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。 0
Human, Mouse and Rat Phospho-SHC (Tyr427) and Total SHC ELISA Kit (96-Well Strip Microplate)
10日程度 ※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。 0
Human, Mouse and Rat Phospho-SHC (Tyr427) and Total SHC ELISA Kit (96-Well Strip Microplate)
10日程度 ※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。 0

[在庫・価格 :2024年04月30日 00時00分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。

Human, Mouse and Rat Phospho-SHC (Tyr427) and Total SHC ELISA Kit (96-Well Strip Microplate)

文献数: 0

Human, Mouse and Rat Phospho-SHC (Tyr427) and Total SHC ELISA Kit (96-Well Strip Microplate)

文献数: 0

Human, Mouse and Rat Phospho-SHC (Tyr427) and Total SHC ELISA Kit (96-Well Strip Microplate)

文献数: 0

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製品説明

■Product Features

  • Rapidly measure phosphorylated protein in lysates
  • Screen numerous different cell lysates without performing a Western Blot analysis
  • Minimal hands-on time, convenient, and non-radioactive material

■Specificity

The antibody pair provided in this kit recognizes Human, Mouse and Rat SHC phosphorylated at site Tyrosine-427 as well as total SHC.

■Target Protein Information

Synonyms: SHC-transforming protein 1 (SHC-transforming protein 3) (SHC-transforming protein A) (Src homology 2 domain-containing-transforming protein C1) (SH2 domain protein C1)

Accession Number: P29353; P98083; O70143

Gene Names: SHC1 SHC SHCA

Gene ID: 6464

■Application Notes

Kit Components

  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Anti-Phospho Antibody
  • HRP-Conjugated Secondary Antibody
  • Assay Diluent
  • TMB One-Step Substrate
  • Stop Solution
  • Lysis Buffer
  • Positive Control Sample

Other Materials Required

  • Distilled or deionized water
  • 100 ml and 1 liter graduated cylinders
  • Tubes to prepare sample dilutions
  • Protease and Phosphatase inhibitors
  • Precision pipettes to deliver 2 µl to 1 ml volumes
  • Adjustable 1-25 ml pipettes for reagent preparation
  • Benchtop rocker or shaker
  • Microplate reader capable of measuring absorbance at 450 nm

Protocol Outline

  1. Prepare all reagents and samples as instructed in the manual.
  2. Add 100 µl of sample or positive control to each well.
  3. Incubate 2.5 h at RT or O/N at 4 °C.
  4. Add 100 µl of prepared primary antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared 1X HRP-Streptavidin to each well.
  7. Incubate 1 h at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

■Typical Data

Positive Control

A431 cells were treated with EGF. Solubilize cells at 4 x 10^7 cells/ml in Cell Lysate Buffer. Serial dilutions of lysates were analyzed in this ELISA. Please see step 3 of Part VI Reagent Preparation for detail.

EGF Stimulation of A431 Cell Lines

A431 cells were treated or untreated with EGF. Cell lysates were analyzed using this phosphoELISA and Western Blot.


■Storage/Stability

Upon receipt, the kit should be stored at –20°C. Please use within 6 months from the date of shipment.

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製品詳細

■ Antigen Information

Gene Symbol:

  • SHC1

■ Product Specifications

Size: 1, 2, or 5 x 96-Well Strip Microplate Kit

■ Array/ELISA Format

Pathway:

  • B Cell Receptor
  • HER/ErbB Family
  • MAPK Signaling

Species Detected:

  • Human
  • Mouse
  • Rat

Solid Support: 96-well Microplate

Design Principle:

  • Sandwich-based

Quantitative/Semi-Quantitative: Semi-Quantitative

Method of Detection: Colorimetric

Compatible Sample Types:

  • Cell Lysates
  • Tissue Lysates

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お問い合わせ先

(テクニカルサポート 試薬担当)

reagent@funakoshi.co.jp

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