Phospho-RSK1(S380), Human, ELISA Kit
掲載日情報:2017/06/21 現在Webページ番号:204116
RayBiotech社のPhospho-RSK1(S380), Human, ELISA Kitです。
※本製品は研究用です。研究用以外には使用できません。
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価格
[在庫・価格 :2024年05月04日 00時00分現在]
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[在庫・価格 :2024年05月04日 00時00分現在]
Phospho-RSK1(S380), Human, ELISA Kit, RayBio
文献数: 0
- 商品コード:PEL-RSK1-S380-1
- メーカー:RAY
- 包装:1kit
- 価格:¥114,000
- 在庫:無(未発注)
- 納期:10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等:
説明文 |
種交差性:Human,アッセイ法:Sandwich-based,検出方法:呈色,測定試料:細胞ライセート, 組織抽出物 別名:RPS6KA1 MAPKAPK1A RSK1 |
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法規制等 | |||
保存条件 | 法規備考 | ||
掲載カタログ |
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製品記事 |
RayBio Phosphorylation ELISA Kit for Cell Lysates Phosphorylation ELISA Kit(RayBiotech社) 翻訳後修飾(PTM)タンパク質研究用アレイ/ELISAキット製品(RayBiotech社) |
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関連記事 |
Phospho-RSK1(S380), Human, ELISA Kit, RayBio
文献数: 0
- 商品コード:PEL-RSK1-S380-2
- メーカー:RAY
- 包装:2kits
- 価格:¥204,000
- 在庫:無(未発注)
- 納期:10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等:
説明文 |
種交差性:Human,アッセイ法:Sandwich-based,検出方法:呈色,測定試料:細胞ライセート, 組織抽出物 別名:RPS6KA1 MAPKAPK1A RSK1 |
||
---|---|---|---|
法規制等 | |||
保存条件 | 法規備考 | ||
掲載カタログ |
|
||
製品記事 |
RayBio Phosphorylation ELISA Kit for Cell Lysates Phosphorylation ELISA Kit(RayBiotech社) 翻訳後修飾(PTM)タンパク質研究用アレイ/ELISAキット製品(RayBiotech社) |
||
関連記事 |
Human Phosph-RSK1 (S380) ELISA Kit
文献数: 0
- 商品コード:PEL-RSK1-S380-5
- メーカー:RAY
- 包装:5kits
- 価格:¥451,000
- 在庫:無(未発注)
- 納期:10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等:
説明文 |
種交差性:Human,アッセイ法:Sandwich-based,検出方法:呈色,測定試料:細胞ライセート, 組織抽出物 別名:RPS6KA1 MAPKAPK1A RSK1 |
||
---|---|---|---|
法規制等 | |||
保存条件 | 法規備考 | ||
掲載カタログ |
|
||
製品記事 |
RayBio Phosphorylation ELISA Kit for Cell Lysates Phosphorylation ELISA Kit(RayBiotech社) |
||
関連記事 |
追加しました。
製品説明
■Introduction
RayBio® Phospho- RSK1 (S380) ELISA kit is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates. By determining phosphorylated RSK1 protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.
This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human phospho-RSK1. An anti-pan RSK1 antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and RSK1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and rabbit anti-RSK1 (S380) antibody is used to detect phosphorylated RSK1. After washing away unbound antibody, HRP-conjugated anti-rabbit IgG is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of RSK1 (S380) bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
■Product Features
- Rapidly measure phosphorylated protein in lysates
- Screen numerous different cell lysates without performing a Western Blot analysis
- Minimal hands-on time, convenient, and non-radioactive material
■Specificity
The antibody pair provided in this kit recognizes human RSK1 phosphorylated at site Serine-380 .
■Target Protein Information
Synonyms: Ribosomal protein S6 kinase alpha-1 (S6K-alpha-1) (EC 2.7.11.1) (90 kDa ribosomal protein S6 kinase 1) (p90-RSK 1) (p90RSK1) (p90S6K) (MAP kinase-activated protein kinase 1a) (MAPK-activated protein kinase 1a) (MAPKAP kinase 1a) (MAPKAPK-1a) (Ribosomal S6 kinase 1) (RSK-1)
Accession Number: Q15418
Gene Names: RPS6KA1 MAPKAPK1A RSK1
Gene ID: 6195
■Species Detected
Human
■Application Notes
Kit Components
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Anti-Phospho Antibody
- HRP-Conjugated Secondary Antibody
- Assay Diluent
- TMB One-Step Substrate
- Stop Solution
- Lysis Buffer
- Positive Control Sample
Other Materials Required
- Distilled or deionized water
- 100 ml and 1 liter graduated cylinders
- Tubes to prepare sample dilutions
- Protease and Phosphatase inhibitors
- Precision pipettes to deliver 2 µl to 1 ml volumes
- Adjustable 1-25 ml pipettes for reagent preparation
- Benchtop rocker or shaker
- Microplate reader capable of measuring absorbance at 450 nm
Protocol Outline
- Prepare all reagents and samples as instructed in the manual.
- Add 100 µl of sample or positive control to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 µl of prepared primary antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared 1X HRP-Streptavidin to each well.
- Incubate 1 h at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
■Typical Data
Positive Control
A431 cells were treated with EGF at 37°C for 20 min. Cells were lysed in Cell Lysate Buffer (4 x 10^7cells/ml). Serial dilutions of lysates were analyzed using the RayBio Human Phospho-RSK1 (S380) ELISA. Please see protocol (Part VI, Reagent Preparation) for details.
EGF Stimulation of A431 Cell Lines
A431 cells were treated or untreated with 100ng/ml EGF for 20 min. Cell lysates were analyzed using the RayBio Human Phospho-RSK1 (S380) ELISA and Western Blot.
■Storage/Stability
Upon receipt, the kit should be stored at –20°C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4°C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge, and store at –20°C. Item D, store at 2-8°C for up to one month (store at -20°C for up to 6 months, avoid repeated freeze-thaw cycles). Reconstituted Positive Control (Item K) should be stored at -70°C.
追加しました。
製品詳細
■ Antigen Information
Gene Symbol:
- RPS6KA1
■ Product Specifications
Size: 1, 2, or 5 x 96-Well Strip Microplate Kit
■ Array/ELISA Format
Pathway:
- Apoptosis
- DNA Damage
- MAPK Signaling
Species Detected:
- Human
Solid Support: 96-well Microplate
Design Principle:
- Sandwich-based
Quantitative/Semi-Quantitative: Semi-Quantitative
Method of Detection: Colorimetric
Compatible Sample Types:
- Cell Lysates
- Tissue Lysates
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