Phospho-P27/Kip1(T198), Human, ELISA Kit
掲載日情報:2017/06/21 現在Webページ番号:204087
RayBiotech社のPhospho-P27/Kip1(T198), Human, ELISA Kitです。
※本製品は研究用です。研究用以外には使用できません。
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価格
[在庫・価格 :2024年05月03日 00時01分現在]
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Phospho-P27/Kip1(T198), Human, ELISA Kit |
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Human Phospho-P27/Kip1 (T198) ELISA Kit |
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[在庫・価格 :2024年05月03日 00時01分現在]
Phospho-P27/Kip1(T198), Human, ELISA Kit
文献数: 0
- 商品コード:PEL-P27-T198-1
- メーカー:RAY
- 包装:1kit
- 価格:¥124,000
- 在庫:無(未発注)
- 納期:10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等:
説明文 |
種交差性:Human,アッセイ法:Sandwich-based,検出方法:呈色,測定試料:細胞ライセート, 組織抽出物 別名:PSMD9 |
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法規制等 | |||
保存条件 | 法規備考 | ||
掲載カタログ |
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製品記事 |
RayBio Phosphorylation ELISA Kit for Cell Lysates Phosphorylation ELISA Kit(RayBiotech社) 翻訳後修飾(PTM)タンパク質研究用アレイ/ELISAキット製品(RayBiotech社) |
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関連記事 |
Phospho-P27/Kip1(T198), Human, ELISA Kit
文献数: 0
- 商品コード:PEL-P27-T198-2
- メーカー:RAY
- 包装:2kits
- 価格:¥222,000
- 在庫:無(未発注)
- 納期:10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等:
説明文 |
種交差性:Human,アッセイ法:Sandwich-based,検出方法:呈色,測定試料:細胞ライセート, 組織抽出物 別名:PSMD9 |
||
---|---|---|---|
法規制等 | |||
保存条件 | 法規備考 | ||
掲載カタログ |
|
||
製品記事 |
RayBio Phosphorylation ELISA Kit for Cell Lysates Phosphorylation ELISA Kit(RayBiotech社) 翻訳後修飾(PTM)タンパク質研究用アレイ/ELISAキット製品(RayBiotech社) |
||
関連記事 |
Human Phospho-P27/Kip1 (T198) ELISA Kit
文献数: 0
- 商品コード:PEL-P27-T198-5
- メーカー:RAY
- 包装:5kits
- 価格:¥491,000
- 在庫:無(未発注)
- 納期:10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等:
説明文 |
種交差性:Human,アッセイ法:Sandwich-based,検出方法:呈色,測定試料:細胞ライセート, 組織抽出物 別名:PSMD9 |
||
---|---|---|---|
法規制等 | |||
保存条件 | 法規備考 | ||
掲載カタログ |
|
||
製品記事 |
RayBio Phosphorylation ELISA Kit for Cell Lysates Phosphorylation ELISA Kit(RayBiotech社) 翻訳後修飾(PTM)タンパク質研究用アレイ/ELISAキット製品(RayBiotech社) |
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関連記事 |
追加しました。
製品説明
■Introduction
RayBio® Phospho-P27/Kip1 (T198) ELISA kit is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates. By determining phosphorylated P27/Kip1 protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.
This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human phospho-P27/Kip1. An anti-pan P27/Kip1 antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and P27/Kip1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and rabbit anti-P27/Kip1 (T198) antibody is used to detect phosphorylated P27/Kip1. After washing away unbound antibody, HRP-conjugated anti-rabbit IgG is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of P27/Kip1 (T198) bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
■Product Features
- Rapidly measure phosphorylated protein in lysates
- Screen numerous different cell lysates without performing a Western Blot analysis
- Minimal hands-on time, convenient, and non-radioactive material
■Specificity
The antibody pair provided in this kit recognizes human p27/Kip1 Phosphorylated at site Threonine-198.
■Target Protein Information
Synonyms: 26S proteasome non-ATPase regulatory subunit 9 (26S proteasome regulatory subunit p27)
Accession Number: O00233
Gene Names: PSMD9
Gene ID: 5715
■Species Detected
Human
■Application Notes
Kit Components
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Anti-Phospho Antibody
- HRP-Conjugated Secondary Antibody
- Assay Diluent
- TMB One-Step Substrate
- Stop Solution
- Lysis Buffer
- Positive Control Sample
Other Materials Required
- Distilled or deionized water
- 100 ml and 1 liter graduated cylinders
- Tubes to prepare sample dilutions
- Protease and Phosphatase inhibitors
- Precision pipettes to deliver 2 µl to 1 ml volumes
- Adjustable 1-25 ml pipettes for reagent preparation
- Benchtop rocker or shaker
- Microplate reader capable of measuring absorbance at 450 nm
Protocol Outline
- Prepare all reagents and samples as instructed in the manual.
- Add 100 µl of sample or positive control to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 µl of prepared primary antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared 1X HRP-Streptavidin to each well.
- Incubate 1 h at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
■Typical Data
Positive Control
Jurkat cells were treated with H2O2 at 37°C for 2 min. Solubilize cells at 4 x 107 cells/ml in Cell Lysate Buffer. Serial dilutions of lysates were analyzed in this ELISA. Please see step 3 of Part VI Reagent Preparation for detail.
H2O2 Stimulation of Jurkat Cell Lines
Jurkat cells were treated or untreated with 2mM H2O2 for 2 min. Cell lysates were analyzed using this phosphoELISA and Western Blot.
■Storage/Stability
Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
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製品詳細
■ Antigen Information
Gene Symbol:
- PSMD9
■ Product Specifications
Size: 1, 2, or 5 x 96-Well Strip Microplate Kit
■ Array/ELISA Format
Pathway:
- Cell Cycle
Species Detected:
- Human
Solid Support: 96-well Microplate
Design Principle:
- Sandwich-based
Quantitative/Semi-Quantitative: Semi-Quantitative
Method of Detection: Colorimetric
Compatible Sample Types:
- Cell Lysates
- Tissue Lysates
追加しました。
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