Phospho-H2AX(S139) and Total H2AX, Human, ELISA Kit
掲載日情報:2017/06/21 現在Webページ番号:204043
RayBiotech社のPhospho-H2AX(S139) and Total H2AX, Human, ELISA Kitです。
※本製品は研究用です。研究用以外には使用できません。
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価格
[在庫・価格 :2024年04月29日 15時15分現在]
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[在庫・価格 :2024年04月29日 15時15分現在]
Phospho-H2AX(S139) and Total H2AX, Human, ELISA Kit
文献数: 0
- 商品コード:PEL-H2AX-S139-T-1
- メーカー:RAY
- 包装:1kit
- 価格:¥124,000
- 在庫:無(未発注)
- 納期:10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等:
説明文 |
種交差性:Human,アッセイ法:Sandwich-based,検出方法:呈色,測定試料:細胞ライセート, 組織抽出物 別名:H2AFX H2AX |
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法規制等 | |||
保存条件 | -20℃ | 法規備考 | |
掲載カタログ |
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製品記事 |
RayBio Phosphorylation ELISA Kit for Cell Lysates Phosphorylation ELISA Kit(RayBiotech社) 翻訳後修飾(PTM)タンパク質研究用アレイ/ELISAキット製品(RayBiotech社) |
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関連記事 |
Phospho-H2AX(S139) and Total H2AX, Human, ELISA Kit
文献数: 0
- 商品コード:PEL-H2AX-S139-T-2
- メーカー:RAY
- 包装:2kits
- 価格:¥222,000
- 在庫:無(未発注)
- 納期:10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等:
説明文 |
種交差性:Human,アッセイ法:Sandwich-based,検出方法:呈色,測定試料:細胞ライセート, 組織抽出物 別名:H2AFX H2AX |
||
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法規制等 | |||
保存条件 | 法規備考 | ||
掲載カタログ |
|
||
製品記事 |
RayBio Phosphorylation ELISA Kit for Cell Lysates Phosphorylation ELISA Kit(RayBiotech社) 翻訳後修飾(PTM)タンパク質研究用アレイ/ELISAキット製品(RayBiotech社) |
||
関連記事 |
Human Phospho-H2AX (S139) and Total H2AX ELISA Kit
文献数: 0
- 商品コード:PEL-H2AX-S139-T-5
- メーカー:RAY
- 包装:5kits
- 価格:¥491,000
- 在庫:無(未発注)
- 納期:10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等:
説明文 |
種交差性:Human,アッセイ法:Sandwich-based,検出方法:呈色,測定試料:細胞ライセート, 組織抽出物 別名:H2AFX H2AX |
||
---|---|---|---|
法規制等 | |||
保存条件 | 法規備考 | ||
掲載カタログ |
|
||
製品記事 |
RayBio Phosphorylation ELISA Kit for Cell Lysates Phosphorylation ELISA Kit(RayBiotech社) 翻訳後修飾(PTM)タンパク質研究用アレイ/ELISAキット製品(RayBiotech社) |
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関連記事 |
追加しました。
製品説明
■Introduction
RayBio® Phospho-H2AX (S139) and Total H2AX ELISA kit is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates. By determining phosphorylated H2AX protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.
This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human phospho-H2AX and total H2AX. An anti-pan H2AX antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and H2AX present in a sample is bound to the wells by the immobilized antibody. The wells are washed and rabbit anti-H2AX (S139) antibody is used to detect phosphorylated H2AX or rabbit anti-H2AX antibody is used to detect pan H2AX. After washing away unbound antibody, HRP-conjugated anti-rabbit IgG is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of H2AX (S139) or pan H2AX bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
■Product Features
- Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
- Screen numerous different cell lysates without performing a Western Blot analysis
- Minimal hands-on time, convenient, and non-radioactive material
■Specificity
The antibody pair provided in this kit recognizes human H2AX phosphorylated at Serine-139 and total H2AX.
■Target Protein Information
Synonyms: Histone H2AX (H2a/x) (Histone H2A.X)
Accession Number: P16104
Gene Names: H2AFX H2AX
Gene ID: 3014
■Species Detected
Human
■Application Notes
Kit Components
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Anti-Phospho Antibody
- Anti-Pan Antibody
- HRP-Conjugated Secondary Antibody
- Streptavidin-Conjugated HRP
- Assay Diluent
- TMB One-Step Substrate
- Stop Solution
- Lysis Buffer
- Positive Control Sample
Other Materials Required
- Distilled or deionized water
- 100 ml and 1 liter graduated cylinders
- Tubes to prepare sample dilutions
- Protease and Phosphatase inhibitors
- Precision pipettes to deliver 2 µl to 1 ml volumes
- Adjustable 1-25 ml pipettes for reagent preparation
- Benchtop rocker or shaker
- Microplate reader capable of measuring absorbance at 450 nm
Protocol Outline
- Prepare all reagents and samples as instructed in the manual.
- Add 100 µl of sample or positive control to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 µl of prepared primary antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared 1X HRP-Streptavidin to each well.
- Incubate 1 h at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
■Typical Data
Positive Control
Jurkat cells were treated with CPT at 37°C for 16 hours. Solubilize cells at 4 x 10^7 cells/ml in Cell Lysate Buffer. Serial dilutions of lysates were analyzed in this ELISA. Please see step 3 of Part VI Reagent Preparation for detail.
CPT Stimulation of Jurkat Cell Lines
Jurkat cells were treated or untreated with CPT for 16 hours. Cell lysates were analyzed using this phosphoELISA and Western Blot.
■Storage/Stability
Upon receipt, the kit should be stored at –20°C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4°C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge, and store at –20°C. Item D, store at 2-8°C for up to one month (store at -20°C for up to 6 months, avoid repeated freeze-thaw cycles). Reconstituted Positive Control (Item K) should be stored at -70°C.
追加しました。
製品詳細
■ Antigen Information
Gene Symbol:
- H2AFX
■ Product Specifications
Size: 1, 2, or 5 x 96-Well Strip Microplate Kit
■ Array/ELISA Format
Pathway:
- DNA Damage
Species Detected:
- Human
Solid Support: 96-well Microplate
Design Principle:
- Sandwich-based
Quantitative/Semi-Quantitative: Semi-Quantitative
Method of Detection: Colorimetric
Compatible Sample Types:
- Cell Lysates
- Tissue Lysates
追加しました。
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