抗Mouse CD6抗体(Anti-Mouse CD6 antibody)
掲載日情報：2021/01/28 現在Webページ番号：199174
Mouse CD6に対する抗体(Anti-Mouse CD6 )です。
※ 本製品は研究用です。研究用以外には使用できません。
追加しました。
- 価格
- Product Details
- Applications and Data
- References
- Related Research Areas
- Related Product & Information
価格
[在庫・価格 ：2024年05月16日 19時35分現在]
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Anti-Mouse CD6 MAb (Clone 96117) |
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Anti-Mouse CD6 MAb (Clone 96117) |
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[在庫・価格 ：2024年05月16日 19時35分現在]
Anti-Mouse CD6 MAb (Clone 96117)
文献数： 0
- 商品コード：MAB7271
- メーカー：RSD
- 包装：500μg
- 価格：¥121,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 |
マッチドペア：Mouse CD6 サンドイッチELISAの補足用抗体として利用可能，検出用抗体として#BAM727，スタンダードとして#727-CD-100を用いる。 別名：CD6 クローン：96117 Genbank No： 923 Protein Accession No： Q61003 |
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| 別包装品 | 別包装品あり | ||||||
| 法規制等 | |||||||
| 保存条件 | -20℃ | 法規備考 | |||||
| 抗原種 | Mouse | 免疫動物 | Rat | ||||
| 交差性 | Mouse | 適用 | Blocking,ELISA | ||||
| 標識 | Unlabeled | 性状 | Protein A/G Affinity Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Monoclonal | フォーマット | |||||
| 掲載カタログ |
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| 製品記事 |
Proteome Profiler™ Human Hematopoietic and Non-hematopoietic Soluble Receptor Antibody Arrays |
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| 関連記事 |
R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Anti-Mouse CD6 MAb (Clone 96117)
文献数： 0
- 商品コード：MAB7271-SP
- メーカー：RSD
- 包装：25μg
- 価格：¥30,000
- 在庫：無(未発注)
- 納期：2～3週間 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 |
※受注発注品。形状：溶液または凍結乾燥 別名：CD6 クローン：96117 Genbank No： 923 Protein Accession No： Q61003 |
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|---|---|---|---|---|---|---|---|
| 別包装品 | 別包装品あり | ||||||
| 法規制等 | |||||||
| 保存条件 | -20℃ | 法規備考 | |||||
| 抗原種 | 免疫動物 | Rat | |||||
| 交差性 | Mouse | 適用 | Blocking,ELISA | ||||
| 標識 | Unlabeled | 性状 | Protein A/G Affinity Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Monoclonal | フォーマット | |||||
| 掲載カタログ |
|
||||||
| 製品記事 | |||||||
| 関連記事 |
R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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追加しました。
Product Details
| Species Reactivity | Mouse |
|---|---|
| Label | Unconjugated |
| Immunogen | Mouse myeloma cell line NS0-derived recombinant mouse CD6Leu18-Gly396Accession # Q61003 |
| Source | Monoclonal Rat IgG1 Clone # 96117 |
| Purification | Protein A or G purified from hybridoma culture supernatant |
| Specificity | Detects mouse CD6 in ELISAs. In sandwich immunoassays, no cross-reactivity or interference was observed with recombinant human CD6 or recombinant mouse ALCAM. |
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Applications and Data
| Recommended Concentration | Sample | |
| Adhesion Blockade | The adhesion of HuT 78 human cutaneous T cell lymphoma cells (5 x 104 cells/well) to immobilized Recombinant mouse CD6 Fc Chimera (Catalog # 727-CD, 10 µg/mL, 100 µL/well) was maximally inhibited (80-100%) by 3 µg/mL of the antibody. | |
| Mouse CD6 Sandwich Immunoassay | Reagent | |
| ELISA Capture (Matched Antibody Pair) | 2-8 µg/mL | Mouse CD6 Antibody (Catalog #MAB7271 ) |
| ELISA Detection (Matched Antibody Pair) | 0.5-2.0 µg/mL | Mouse CD6 Biotinylated Antibody (Catalog #BAM727 ) |
| ELISA Standard | Recombinant Mouse CD6 Fc Chimera Protein, CF (Catalog #727-CD ) | |
| Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website. | Preparation and Storage | |
| Reconstitution | Reconstitute at 0.5 mg/mL in sterile PBS. | Reconstitution Buffer Available |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C | |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. | |
| 1 month, 2 to 8 °C under sterile conditions after reconstitution. | ||
| 6 months, -20 to -70 °C under sterile conditions after reconstitution. | ||
| Background: CD6 | CD6 is a member of the group B scavenger receptor cysteine-rich (SRCR) superfamily. CD6 is a type I membrane glycoprotein and contains three extracellular SRCR domains. CD6 is expressed at low levels on immature thymocytes and at high levels on mature thymocytes. The majority of peripheral blood T cells, a subset of B cells, and a subset of neuronal cells express CD6. Mouse CD6 is a 626 amino acid (aa) protein with a 24 aa sequence, a 372 aa extracellular domain, and a 204 aa cytoplasmic region. The 668 aa human homolog has also been identified. The human and murine proteins share 70% aa identity over their full-lengths. The role of CD6 has not been fully elucidated. However, it appears to play a role as both a co-stimulatory molecule in T cell activation and as an adhesion receptor. Studies demonstrating a mitogenic effect for T cells with some CD6 specific monoclonal antibodies, in conjunction with either accessory cells or PMA and anti-CD2 mAb, support the concept of CD6 as a co-stimulatory molecule. Additionally, anti-CD6 monoclonal antibody has been used as an immunosuppressive agent for patients undergoing kidney or bone marrow allograft rejection. It has also been used to remove CD6+ T cells from donor bone marrow prior to allogenic bone marrow transplantation. Other studies have demonstrated an adhesive role for CD6, it has been demonstrated to bind the activated leukocyte cell adhesion molecule (ALCAM, CD166). CD6/ALCAM interactions have been postulated to play a role in thymocyte development. Additionally, the presence of ALCAM on neuronal cells may provide a mechanism of interaction between CD6+ T cell and ALCAM+ neuronal cells. Phosphorylation of the CD6 molecule appears to play a role in CD6-mediated signal transduction. Serine and threonine residues become hyperphosphorylated and tyrosine residues become phosphorylated when T cells are activated with anti-CD6 mAb in conjunction with PMA, anti-CD2, or anti-CD3 mAb. The CD6 intracellular domain contains regions that can interact with SH2 or SH3 containing proteins. However, the signaling pathways have not been elucidated. |
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References
| Gangemi, R. et al. (1989) J. Immunol. 143:2439. | |
| Aruffo, A. et al. (1991) J. Exp. Med. 174:949. | |
| Swack, J.A. et al. (1991) J. Biol. Chem. 266:7137. | |
| Robinson, W.H. et al. (1995) Eur. J. Immunol. 25:2765. | |
| Whitney, G. et al. (1995) Mol. Immunol. 32:89. | |
| Starling, G.C. et al. (1996) Eur. J. Immunol. 26:738. | |
| Degen, W.G. et al. (1998) Am. J. Pathol. 152:805. | |
| Swack, J.A. et al. (1989) Mol. Immunol. 26:1037. | |
| Pawelec, G. and H.J. Buhring (1991) Human Immunol. 31:165. | |
| Osorio, L.M. et al. (1995) Cell Immunol. 166:44. | |
| Robinson, W.H. et al. (1995) J. Immunol. 155:4739. | |
| Singer, N.G. et al. (1996) Immunology 88:537. | |
| Aruffo, A. et al. (1997) Immunol. Today 18:498. | |
| Entrez Gene IDs: | 923 (Human); 12511 (Mouse) |
| Alternate Names: | CD6 antigenFLJ44171; CD6 molecule; CD6; T12; Tp120; TP120T-cell differentiation antigen CD6 |
追加しました。
Related Research Areas
| ITIM/ITAM Immunoreceptors and Related Molecules |
| Leukocyte Receptors/Markers in Allergy and Asthma |
| Scavenger Receptors |
| T Cell Migration/Adhesion |
追加しました。
Related Product & Information
| Entrez Gene IDs | 923 (Human); 12511 (Mouse) |
|---|---|
| Background | CD6 |
| background_content | Background: CD6 CD6 is a member of the group B scavenger receptor cysteine-rich (SRCR) superfamily. CD6 is a type I membrane glycoprotein and contains three extracellular SRCR domains. CD6 is expressed at low levels on immature thymocytes and at high levels on mature thymocytes. The majority of peripheral blood T cells, a subset of B cells, and a subset of neuronal cells express CD6. Mouse CD6 is a 626 amino acid (aa) protein with a 24 aa sequence, a 372 aa extracellular domain, and a 204 aa cytoplasmic region. The 668 aa human homolog has also been identified. The human and murine proteins share 70% aa identity over their full-lengths. The role of CD6 has not been fully elucidated. However, it appears to play a role as both a co-stimulatory molecule in T cell activation and as an adhesion receptor. Studies demonstrating a mitogenic effect for T cells with some CD6 specific monoclonal antibodies, in conjunction with either accessory cells or PMA and anti-CD2 mAb, support the concept of CD6 as a co-stimulatory molecule. Additionally, anti-CD6 monoclonal antibody has been used as an immunosuppressive agent for patients undergoing kidney or bone marrow allograft rejection. It has also been used to remove CD6+ T cells from donor bone marrow prior to allogenic bone marrow transplantation. Other studies have demonstrated an adhesive role for CD6, it has been demonstrated to bind the activated leukocyte cell adhesion molecule (ALCAM, CD166). CD6/ALCAM interactions have been postulated to play a role in thymocyte development. Additionally, the presence of ALCAM on neuronal cells may provide a mechanism of interaction between CD6+ T cell and ALCAM+ neuronal cells. Phosphorylation of the CD6 molecule appears to play a role in CD6-mediated signal transduction. Serine and threonine residues become hyperphosphorylated and tyrosine residues become phosphorylated when T cells are activated with anti-CD6 mAb in conjunction with PMA, anti-CD2, or anti-CD3 mAb. The CD6 intracellular domain contains regions that can interact with SH2 or SH3 containing proteins. However, the signaling pathways have not been elucidated. |
追加しました。
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