抗Human IL-1 beta/IL-1F2 Propeptide抗体(Anti-Human IL-1 beta/IL-1F2 Propeptide antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	E. coli-derived recombinant human IL-1 beta /IL-1F2 PropeptideMet1-Asp116Accession # P01584
Source	Monoclonal Mouse IgG1 Clone # 615417
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects human IL-1 beta /IL-1F2 Propeptide in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) IL-1 alpha, rhIL-1F7, rhIL-1F10, rhIL-18, rhIL-36 alpha, rhIL-36 beta, IL-36 gamma, rhIL-36Ra, recombinant mouse IL-1Ra, or the mature peptides of recombinant IL-1 beta from canine, cotton rat, equine, feline, guinea pig, human, mouse, porcine, rabbit, rat, or rhesus is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	2 µg/mL	See below
Simple Western	10 µg/mL	See below
Immunocytochemistry	8-25 µg/mL	See below

Western Blot
	Detection of Human IL‑1 beta /IL‑1F2 Propeptide by Western Blot. Western blot shows lysates of THP‑1 human acute monocytic leukemia cell line untreated(-) or treated (+) with 80 nM PMA for 48 hours. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human IL‑1 beta /IL‑1F2 Propeptide Monoclonal Antibody (Catalog # MAB6964) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for IL‑1 beta /IL‑1F2 Propeptide at approximately 34 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Immunocytochemistry
	IL‑1 beta /IL‑1F2 in Human PBMCs. IL‑1 beta /IL‑1F2 was detected in immersion fixed human peripheral blood mononuclear cells using Mouse Anti-Human IL‑1 beta /IL‑1F2 Propeptide Monoclonal Antibody (Catalog # MAB6964) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI(blue). Specific staining was localized to cytoplasmic. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Simple Western
	Detection of Human IL‑1 beta /IL‑1F2 Propeptide by Simple WesternTM. Simple Western lane view shows lysates of THP‑1 human acute monocytic leukemia cell line untreated (-) or treated (+) with PMA and LPS, loaded at 0.2 mg/mL. A specific band was detected for IL‑1 beta /IL‑1F2 Propeptide at approximately 40 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human IL‑1 beta /IL‑1F2 Propeptide Monoclonal Antibody (Catalog # MAB6964). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

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Related Product & Information

Long Name	Interleukin 1 beta
Background	IL-1 beta/IL-1F2
background_content	Background: IL-1 beta/IL-1F2 IL-1 beta, also known as IL1F2, is a prototypical member of the IL-1 superfamily. It is produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. The biological activity of IL-1 beta is mediated by the heterodimeric receptor complex containing IL-1 R1 (IL-1 RI) and IL-1 R3 (IL-1 RAcP). The IL-1 beta propeptide is cleaved intracellularly by caspase-1/ICE to generate a 17 kDa active cytokine. The propeptide of human IL-1 beta shares approximately 55% aa sequence identity with the propeptides of mouse and rat IL-1 beta.

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Citations

1. MicroRNA-9 Inhibits NLRP3 Inflammasome Activation in Human Atherosclerosis Inflammation Cell Models through the JAK1/STAT Signaling Pathway
   Authors: Y Wang, Z Han, Y Fan, J Zhang, K Chen, L Gao, H Zeng, J Cao, C Wang
   Cell. Physiol. Biochem, 2017;41(4):1555-1571.
   Species: Human
   Sample Type: Cell Culture Supernates
   Application: WB


2. Azithromycin decreases NALP3 mRNA stability in monocytes to limit inflammasome-dependent inflammation
   Authors: EA Lendermon, TA Coon, JS Bednash, NM Weathingto, JF McDyer, RK Mallampall
   Respir. Res., 2017;18(1):131.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


3. NLRP3 inflammasome: a novel link between lipoproteins and atherosclerosis
   Arch Med Sci, 2016;12(5):950-958.
   Species: Human
   Sample Type: Cell Culture Supernates
   Application: WB


4. Propionibacterium acnes Induces IL-1beta secretion via the NLRP3 inflammasome in human monocytes.
   Authors: Qin M, Pirouz A, Kim M, Krutzik S, Garban H, Kim J
   J Invest Dermatol, 2014;134(2):381-8.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


5. Unsaturated fatty acids prevent activation of NLRP3 inflammasome in human monocytes/macrophages.
   Authors: L'homme L, Esser N, Riva L, Scheen A, Paquot N, Piette J, Legrand-Poels S
   J Lipid Res, 2013;54(11):2998-3008.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB

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