抗Mouse IL-17 R抗体(Anti-Mouse IL-17 R antibody)

• 2025/12/01

  試薬 特別価格

  [RSD]厳選 抗体＆リコンビナントタンパク質 30％OFFキャンペーン[～2026/02/27]

  期間：2025/12/01 ～2026/02/27

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  R&D Systems, Inc.（RSD）

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse
Label	Unconjugated
Immunogen	Mouse myeloma cell line NS0-derived recombinant mouse IL‑17 RA/IL‑17 RSer32-Trp322Accession # Q60943
Source	Monoclonal Rat IgG2A Clone # 657603
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects mouse IL‑17 RA/IL‑17 R in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human IL-17 RA, recombinant mouse (rm) IL-17B R, rmIL-17 RD, or rmIL-17 RC is observed. In Western blots, less than 10% cross‑reactivity with recombinant human (rh) IL-17 RA or rhIL-17B R is observed.

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Applications and Data

	Recommended Concentration	Sample
Flow Cytometry	2.5 µg/106 cells	See below
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Neutralization	Measured by its ability to neutralize IL‑17-induced IL-6 secretion in the NIH‑3T3 mouse embryonic fibroblast cell line. Yao, Z. et al. (1995) Immunity 3:811.The Neutralization Dose (ND50) is typically 0.2-0.8 μg/mL in the presence of 10 ng/mL Recombinant Mouse IL‑17.

Flow Cytometry
	Detection of IL‑17 RA/IL‑17 R in EL-4 Mouse Cell Line by Flow Cytometry. EL‑4 mouse lymphoblast cell line was stained with Rat Anti-Mouse IL‑17 RA/IL‑17 R Monoclonal Antibody (Catalog # MAB4481, filled histo­gram) or isotype control antibody (Catalog # MAB006, open histo­gram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113).
Neutralization
	IL-6 secretion Induced by IL‑17 and Neutralization by Mouse IL‑17 RA/IL‑17 R Antibody. Recombinant Mouse IL‑17 (Catalog # 421-ML) induces IL-6 secretion in the NIH‑3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line), as measured by the Mouse IL-6 Quantikine kit (Catalog # M6000B). IL-6 secretion elicited by Recombinant Mouse IL‑17 (10 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse IL‑17 RA/IL‑17 R Monoclonal Antibody (Catalog # MAB4481). The ND50 is typically 0.2‑0.8 μg/mL.

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Related Product & Information

Long Name	Interleukin 17 Receptor
Background	IL-17 RA/IL-17 R
background_content	Background: IL-17 RA/IL-17 R IL-17 receptor alpha subunit (IL-17 RA), also known as IL-17 R, is a widely expressed 120 kDa glycosylated type I transmembrane protein that plays a central role in inflammatory responses. IL‑17 RA associates with IL-17 RC to form a signaling receptor complex for IL-17 and IL-17F. IL-17 RA ligation promotes T cell activation and the production of IL-6, G‑CSF, SCF, and multiple proinflammatory chemokines. Within the ECD, mouse IL-17 RA shares 84% and 72% aa sequence identity with rat and human IL17 RA, respectively.

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Citations

1. Blocking IL-17A Alleviates Diabetic Retinopathy in Rodents
   Authors: AW Qiu, QH Liu, JL Wang
   Cell. Physiol. Biochem, 2017;41(3):960-972.
   Species: Mouse
   Sample Type: In Vivo
   Application: In vivo


2. Interleukin-17A Promotes Lung Tumor Progression Through Neutrophil Attraction to Tumor Sites and Mediating Resistance to PD-1 Blockade
   Authors: EA Akbay, S Koyama, Y Liu, R Dries, LE Bufe, M Silkes, MM Alam, DM Magee, R Jones, M Jinushi, M Kulkarni, J Carretero, X Wang, T Warner-Hat, JD Cavanaugh, A Osa, A Kumanogoh, GJ Freeman, MM Awad, DC Christiani, R Bueno, PS Hammerman, G Dranoff, KK Wong
   J Thorac Oncol, 2017;0(0):.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Neut


3. Keratinocytes contribute intrinsically to psoriasis upon loss of Tnip1 function
   Proc Natl Acad Sci USA, 2016;0(0):.
   Species: Mouse
   Sample Type: In Vivo
   Application: Neut


4. Galectin-3 negatively regulates dendritic cell production of IL-23/IL-17-axis cytokines in infection by Histoplasma capsulatum.
   Authors: Wu S, Yu J, Liu F, Miaw S, Wu-Hsieh B
   J Immunol, 2013;190(7):3427-37.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Neut

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