抗IL-1β抗体(Anti-IL-1β, Mouse-Mono antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse
Label	Unconjugated
Immunogen	E. coli-derived recombinant mouse IL-1 beta Accession # P10749
Source	Monoclonal Hamster IgG Clone # B122
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects mouse IL-1 beta /IL-1 F2 in direct ELISAs and Western blots. In Western blots, shows 100% cross-reactivity with recombinant rat (rr) IL‑1 beta and rcrIL‑1 beta, 15%‑35 % with rhIL‑1 beta and rpIL‑1 beta, and no cross-reactivity with rmIL-1 alpha, rmIL-18, or rhIL-1 beta rp.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	1 µg/mL	Recombinant Mouse IL‑1 beta /IL‑1F2 (Catalog # 401-ML)
Immunoprecipitation	Hogquist, K.A. et al. (1991) J. Immunol. 146:1534.
Neutralization	Measured by its ability to neutralize IL‑1 beta /IL‑1F2-induced proliferation in the D10.G4.1 mouse helper T cell line. Symons, J. A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M. J. et al. (eds): IRL Press. 272. The Neutralization Dose (ND50) is typically 0.3-1.5 µg/mL in the presence of 50 pg/mL Recombinant Mouse IL‑1 beta /IL‑1F2 and 1.25 µg/mL concanavalin A.

Neutralization

Cell Proliferation Induced by IL‑1 beta /IL‑1F2 and Neutralization by Mouse IL‑1 beta /IL‑1F2 Antibody. Recombinant Mouse IL‑1 beta /IL‑1F2 (Catalog # 401-ML) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL‑1 beta /IL‑1F2 (50 pg/mL) is neutralized (green line) by increasing concentrations of Mouse IL‑1 beta /IL‑1F2 Monoclonal Antibody (Catalog # MAB4012). The ND50 is typically 0.3‑1.5 µg/mL in the presence of concanavalin A (1.25 µg/mL).

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Related Product & Information

Long Name	Interleukin 1 beta
Entrez Gene IDs	3553 (Human); 16176 (Mouse); 24494 (Rat); 397122 (Porcine); 403974 (Canine); 100034237 (Equine); 100135556 (Guinea Pig)
Background	IL-1 beta/IL-1F2
background_content	Background: IL-1 beta/IL-1F2 IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 17% amino acid (aa) identity in mouse. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL‑1 R3/IL‑1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI (1‑4). The mouse IL-1 beta cDNA encodes a 269 aa precursor. A 117 aa propeptide is cleaved intracellularly by the cysteine protease IL-1 beta -converting enzyme (Caspase-1/ICE) to generate the active cytokine (5, 6). The 17 kDa mature mouse IL-1 beta shares 90% aa sequence identity with cotton rat and rat and 65%‑78% identity with canine, equine, feline, human, porcine, and rhesus IL-1 beta.

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Citations

1. Key Role of the Scavenger Receptor MARCO in Mediating Adenovirus Infection and Subsequent Innate Responses of Macrophages
   Authors: MD Maler, PJ Nielsen, N Stichling, I Cohen, Z Ruzsics, C Wood, P Engelhard, M Suomalaine, I Gyory, M Huber, J Müller-Que, WWA Schamel, S Gordon, T Jakob, SF Martin, W Jahnen-Dec, UF Greber, MA Freudenber, G Fejer
   MBio, 2017;8(4):.
   Species: Mouse
   Sample Type: Cell Lysates
   Application: WB


2. Cholinergic anti-inflammatory pathway inhibits neointimal hyperplasia by suppressing inflammation and oxidative stress
   Authors: DJ Li, H Fu, J Tong, YH Li, LF Qu, P Wang, FM Shen
   Redox Biol, 2017;15(0):22-33.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC-F


3. Cigarette smoke-induced accumulation of lung dendritic cells is interleukin-1alpha-dependent in mice.
   Authors: Botelho F, Nikota J, Bauer C, Morissette M, Iwakura Y, Kolbeck R, Finch D, Humbles A, Stampfli M
   Respir Res, 2012;13(0):81.
   Species: Mouse
   Sample Type: In Vivo
   Application: Neut


4. IL-1alpha and IL-1beta recruit different myeloid cells and promote different stages of sterile inflammation.
   Authors: Rider P, Carmi Y, Guttman O, Braiman A, Cohen I, Voronov E, White MR, Dinarello CA, Apte RN
   J. Immunol., 2011;187(9):4835-43.
   Species: Mouse
   Sample Type: Cell Lysates
   Application: WB

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