抗BMP-9抗体(Anti-BMP-9, Mouse-Mono antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human, Mouse, Primate
Label	Unconjugated
Immunogen	E. coli-derived recombinant human BMP-9Ser320-Arg429Accession # Q9UK05
Source	Monoclonal Mouse IgG2B Clone # 360107
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects human and primate BMP‑9 in ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human BMP‑1, -1.1, -2, -3, -3b, -4, -5, -6, -7, -8, or -10 is observed. In direct ELISAs, approximately 80% cross-reactivity with recombinant mouse (rm) BMP‑9 and 10% cross‑reactivity with rmBMP-10 is observed.

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Applications and Data

	Recommended Concentration	Sample
Human/Primate BMP-9 Sandwich Immunoassay	Reagent
ELISA Capture (Matched Antibody Pair)	2-8 µg/mL	Human/Mouse/Primate BMP‑9 Antibody (Catalog #MAB3209 )
ELISA Detection (Matched Antibody Pair)	0.1-0.4 µg/mL	Human/Primate BMP‑9 Biotinylated Antibody (Catalog #BAF3209 )
ELISA Standard		Recombinant Human BMP-9 Protein (Catalog #3209-BP )
Neutralization	Measured by its ability to neutralize BMP‑9-induced alkaline phosphatase production in the ATDC5 mouse chondrogenic cell line. The Neutralization Dose (ND50) is typically 0.01-0.04 µg/mL in the presence of 5 ng/mL Recombinant Human BMP‑9.
Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website.

Neutralization

Alkaline Phosphatase Production Induced by BMP‑9 and Neutralization by Human BMP‑9 Antibody. Recombinant Human BMP‑9 (Catalog # 3209‑BP) induces alkaline phosphatase production in the ATDC5 mouse chondrogenic cell line in a dose-dependent manner (orange line). Alkaline phosphatase production elicited by Recombinant Human BMP‑9 (5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human/Mouse/Primate BMP‑9 Monoclonal Antibody (Catalog # MAB3209). The ND50 is typically 0.01-0.04 µg/mL.

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Related Product & Information

Long Name	Bone Morphogenetic Protein 9
Entrez Gene IDs	2658 (Human); 12165 (Mouse)
Background	BMP-9
background_content	Background: BMP-9 Human BMP-9, also known as growth and differentiation factor 2 (GDF-2), is a member of the BMP subgroup of the TGF-beta superfamily proteins that signal through heterodimeric complexes composed of type I and type II BMP receptors. BMP-9 regulates the development and function of a variety of embryonal and adult tissues (1, 2). The human BMP-9 cDNA encodes a 429 amino acid (aa) precursor that includes a 22 aa signal sequence, a 298 aa propeptide, and a 111 aa mature protein (3). Unlike with other BMP family proteins, the propeptide does not interfere with the biological activity of BMP-9 and remains associated with the mature peptide after proteolytic cleavage (4). Human and mouse BMP-9 share 96% aa sequence identity. Within the mature protein, human BMP-9 shares 64% aa sequence identity with human BMP-10 and less than 50% aa sequence identity with other BMPs. BMP-9 is expressed by non-parenchymal cells in the liver, (5, 6) where it promotes lipid metabolism and inhibits glucose production (7). BMP-9 exerts a prolonged hypoglycemic effect which may be due to an enhancement of insulin release (7). BMP-9 interacts with a high affinity specific heteromeric receptor expressed on liver endothelial cells that has been identified as ALK-1 (4-6). In the embryonal CNS, BMP-9 functions in the development and maintenance of the cholinergic neuronal phenotype (8-10). BMP-9 also induces the differentiation of mesenchymal stem cells into the chondrogenic lineage (11, 12). At low concentrations, BMP-9 is a proliferative factor for hematopoietic progenitor cells, but at higher concentrations, it enhances TGF-beta 1 production and inhibits hematopoietic progenitor colony formation (13).

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Citations

1. Identification of rare sequence variation underlying heritable pulmonary arterial hypertension
   Authors: S Gräf, M Haimel, M Bleda, C Hadinnapol, L Southgate, W Li, J Hodgson, B Liu, RM Salmon, M Southwood, RD Machado, JM Martin, CM Treacy, K Yates, LC Daugherty, O Shamardina, D Whitehorn, S Holden, M Aldred, HJ Bogaard, C Church, G Coghlan, R Condliffe, PA Corris, C Danesino, M Eyries, H Gall, S Ghio, HA Ghofrani, JSR Gibbs, B Girerd, AC Houweling, L Howard, M Humbert, DG Kiely, G Kovacs, RV MacKenzie, S Moledina, D Montani, M Newnham, A Olschewski, H Olschewski, AJ Peacock, J Pepke-Zaba, I Prokopenko, CJ Rhodes, L Scelsi, W Seeger, F Soubrier, DF Stein, J Suntharali, EM Swietlik, MR Toshner, DA van Heel, A Vonk Noord, Q Waisfisz, J Wharton, SJ Wort, WH Ouwehand, N Soranzo, A Lawrie, PD Upton, MR Wilkins, RC Trembath, NW Morrell
   Nat Commun, 2018;9(1):1416.
   Species: Human
   Sample Type: Cell Culture Supernates
   Application: ELISA Capture


2. BMP-9 interferes with liver regeneration and promotes liver fibrosis
   Authors: K Breitkopf-, C Meyer, C König, H Gaitantzi, A Addante, M Thomas, E Wiercinska, C Cai, Q Li, F Wan, C Hellerbran, NA Valous, M Hahnel, C Ehlting, JG Bode, S Müller-Boh, U Klingmülle, J Altenöder, I Ilkavets, MJ Goumans, LJ Hawinkels, SJ Lee, M Wieland, C Mogler, MP Ebert, B Herrera, H Augustin, A Sánchez, S Dooley, P Ten Dijke
   Gut, 2017;0(0):.
   Species: Mouse
   Sample Type: Plasma
   Application: ELISA Capture


3. Rapid Activation of Bone Morphogenic Protein 9 by Receptor-mediated Displacement of Pro-domains
   Authors: Y Kienast, U Jucknischk, S Scheiblich, M Thier, M de Wouters, A Haas, C Lehmann, V Brand, D Bernicke, K Honold, S Lorenz
   J. Biol. Chem, 2016;291(7):3395-410.
   Species: Human
   Sample Type: Protein
   Application: ELISA - Capture


4. A mouse model of hereditary hemorrhagic telangiectasia generated by transmammary-delivered immunoblocking of BMP9 and BMP10
   Sci Rep, 2016;5(0):37366.
   Species: Mouse
   Sample Type: In Vivo
   Application: In vivo


5. H3K9 methylation is a barrier during somatic cell reprogramming into iPSCs.
   Authors: Chen, Jiekai, Liu, He, Liu, Jing, Qi, Jing, Wei, Bei, Yang, Jiaqi, Liang, Hanquan, Chen, You, Chen, Jing, Wu, Yaran, Guo, Lin, Zhu, Jieying, Zhao, Xiangjie, Peng, Tianran, Zhang, Yixin, Chen, Shen, Li, Xuejia, Li, Dongwei, Wang, Tao, Pei, Duanqing
   Nat Genet, 2013;45(1):34-42.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Neut


6. Anti-human activin receptor-like kinase 1 (ALK1) antibody attenuates bone morphogenetic protein 9 (BMP9)-induced ALK1 signaling and interferes with endothelial cell sprouting.
   Authors: van Meeteren LA, Thorikay M, Bergqvist S, Pardali E, Stampino CG, Hu-Lowe D, Goumans MJ, ten Dijke P
   J. Biol. Chem., 2012;287(22):18551-61.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


7. Crossveinless 2 regulates bone morphogenetic protein 9 in human and mouse vascular endothelium.
   Authors: Yao Y, Jumabay M, Ly A, Radparvar M, Wang AH, Abdmaulen R, Bostrom KI
   Blood, 2012;119(21):5037-47.
   Species: Human
   Sample Type: Cell Lysates
   Application: IP


8. BMP9 and BMP10 are critical for postnatal retinal vascular remodeling.
   Blood, 2012;119(25):6162-71.
   Species: Mouse
   Sample Type: In Vivo
   Application: In vivo


9. A rapid and sensitive bioassay for the simultaneous measurement of multiple bone morphogenetic proteins. Identification and quantification of BMP4, BMP6 and BMP9 in bovine and human serum.
   Authors: Herrera B, Inman GJ,
   BMC Cell Biol., 2009;10(0):20.
   Species: Human
   Sample Type: Recombinant Protein
   Application: Neut

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