抗Feline CXCL8/IL-8抗体(Anti-Feline CXCL8/IL-8 antibody)
掲載日情報：2021/01/28 現在Webページ番号：196038
Feline CXCL8/IL-8に対する抗体(Anti-Feline CXCL8/IL-8 )です。
※ 本製品は研究用です。研究用以外には使用できません。
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- 価格
- Product Details
- Applications and Data
- Data Examples
- References
- Related Research Areas
- Related Product & Information
価格
[在庫・価格 ：2025年05月01日 15時35分現在]
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Anti-Feline CXCL8/IL-8 MAb (Clone 344615) |
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Anti-Feline CXCL8/IL-8 MAb (Clone 344615) |
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[在庫・価格 ：2025年05月01日 15時35分現在]
Anti-Feline CXCL8/IL-8 MAb (Clone 344615)
文献数： 0
- 商品コード：MAB22772
- メーカー：RSD
- 包装：500μg
- 価格：¥121,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | マッチドペア：Feline CXCL8/IL-8 サンドイッチELISAの補足用抗体として利用可能，検出用抗体として#BAM22771，スタンダードとして#2277-FL-025を用いる。 別名：3-10C クローン：344615 Genbank No： 3576 Protein Accession No： Q9XSX5.1 |
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| 別包装品 | 別包装品あり | ||||||
| 法規制等 | |||||||
| 保存条件 | 法規備考 | ||||||
| 抗原種 | Feline | 免疫動物 | Rat | ||||
| 交差性 | Feline | 適用 | ELISA,Neutralising | ||||
| 標識 | Unlabeled | 性状 | Protein A/G Affinity Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Monoclonal | フォーマット | |||||
| 掲載カタログ |
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| 製品記事 | M1/M2 Macrophage Activation Marker |
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| 関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Anti-Feline CXCL8/IL-8 MAb (Clone 344615)
文献数： 0
- 商品コード：MAB22772-SP
- メーカー：RSD
- 包装：25μg
- 価格：¥30,000
- 在庫：無(未発注)
- 納期：2～3週間 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | ※受注発注品。形状：溶液または凍結乾燥 別名：3-10C クローン：344615 Genbank No： 3576 Protein Accession No： Q9XSX5.1 |
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| 別包装品 | 別包装品あり | ||||||
| 法規制等 | |||||||
| 保存条件 | 法規備考 | ||||||
| 抗原種 | 免疫動物 | Rat | |||||
| 交差性 | Feline | 適用 | ELISA,Neutralising | ||||
| 標識 | Unlabeled | 性状 | Protein A/G Affinity Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Monoclonal | フォーマット | |||||
| 掲載カタログ |
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| 製品記事 | M1/M2 Macrophage Activation Marker 使いっきり抗体 |
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| 関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Product Details
| Species Reactivity | Feline |
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| Label | Unconjugated |
| Immunogen | E. coli-derived recombinant feline IL-8/CXCL8Ala23-Ala101Accession # Q9XSX5.1 |
| Source | Monoclonal Rat IgG2A Clone # 344615 |
| Purification | Protein A or G purified from hybridoma culture supernatant |
| Specificity | Detects feline IL-8/CXCL8 in ELISAs. In sandwich immunoassays, no cross-reactivity with recombinant human IL-8/CXCL8, recombinant canine IL-8/CXCL8, or recombinant porcine IL-8/CXCL8 is observed. |
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Applications and Data
| Recommended Concentration | Sample | |
| Feline IL-8/CXCL8 Sandwich Immunoassay | Reagent | |
| ELISA Capture (Matched Antibody Pair) | 2-8 µg/mL | Feline IL-8/CXCL8 Antibody (Catalog #MAB22772 ) |
| ELISA Detection (Matched Antibody Pair) | 0.5-2.0 µg/mL | Feline IL-8/CXCL8 Biotinylated Antibody (Catalog #BAM22771 ) |
| ELISA Standard | Recombinant Feline IL-8/CXCL8 Protein (Catalog #2277-FL ) | |
| Neutralization | Measured by its ability to neutralize IL-8/CXCL8-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR2. The Neutralization Dose (ND50) is typically 0.075-0.375 µg/mL in the presence of 20 ng/mL Recombinant Feline IL-8/CXCL8. | |
| Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website. | ||
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Data Examples
| Neutralization |
![Neutralization CXCL8/IL-8 Antibody (344615) [Unconjugated]](http://resources.rndsystems.com/images/datasheets/antibody/CXCL8_MAB22772_Block_Neutralize_9085.jpg) click image to view larger | Chemotaxis Induced by IL-8/CXCL8 and Neutralization by Feline IL-8/CXCL8 Antibody.Recombinant Feline IL-8/CXCL8 (Catalog # 2277-FL) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Feline IL-8/CXCL8 (20 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Feline IL-8/CXCL8 Monoclonal Antibody (Catalog # MAB22772). The ND50 is typically 0.075‑0.375 µg/mL. |
| Reconstitution | Reconstitute at 0.5 mg/mL in sterile PBS. | Reconstitution Buffer Available |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C | |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. | |
| 1 month, 2 to 8 °C under sterile conditions after reconstitution. | ||
| 6 months, -20 to -70 °C under sterile conditions after reconstitution. | ||
| Background: IL-8/CXCL8 | Interleukin 8 (IL-8), also named CXCL8, monocyte-derived neutrophil chemotactic factor (MDNCF), neutrophil-activating protein 1 (NAP-1), neutrophil-activating factor (NAF) and granulocyte chemotactic peptide (GCP), belongs to the Glu-Leu-Arg motif containing (ELR+) CXC chemokine family and has been designated CXCL8. IL-8 is a potent neutrophil chemoattractant that recruits neutrophils to sites of inflammation. IL-8 also activates neutrophil functions and promotes angiogenesis. The biological activites of IL-8 is mediated by two types of G protein-coupled chemokine receptors, CXCR1 and CXCR2 (1, 2). In normal tissues, IL-8 expression and secretion is barely detectable. Upon stimulation by a wide range of pro-inflammatory signals including exposure to IL-1, TNF, bacterial or viral products, IL-8 production is rapidly induced in many different cell types (3, 4). Feline IL-8 encodes a 101 amino acid (aa) precursor protein with a putative 22 aa signal peptide. It shares 61% and 76% aa sequence identity with human and canine IL-8, respectively. |
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References
| Van Damme, J. et al. (1998) The Cytokine Handbook, A.W. Thomson, ed., Academic Press, NY p. 271. | |
| Heidemann, J. et al. (2003) J. Biol. Chem. 178:8508. | |
| Yang, M.P. et al. (2002) Vet. Immunol. Immunopathol. 86:43. | |
| Parhar, K. et al. (2003) Immunology 108:502. | |
| Long Name: | Interleukin 8 |
| Entrez Gene IDs: | 3576 (Human); 396880 (Porcine); 403850 (Canine); 493836 (Feline) |
| Alternate Names: | 3-10C; AMCF-I; C-X-C motif chemokine 8; CXCL8; CXCL8SCYB8; Emoctakin; GCP1; GCP-1TSG-1; IL8; IL-8; interleukin 8; K60; LAI; LECT; MDNCF; MDNCFb-ENAP; member 8; MONAPGCP1; NAP1; NAP-1NAP1; NCF; Neutrophil-activating protein 1; Protein 3-10C; T cell chemotactic factor; T-cell chemotactic factor; TCF; TSG1 |
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Related Research Areas
| Neutralization | |
|---|---|
 | Chemotaxis Induced by IL-8/CXCL8 and Neutralization by Feline IL-8/CXCL8 Antibody. Recombinant Feline IL-8/CXCL8 (Catalog # 2277-FL) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant FelineIL-8/CXCL8 (20 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Feline IL-8/CXCL8 Monoclonal Antibody (Catalog # MAB22772). The ND50 is typically0.075‑0.375 µg/mL. |
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Related Product & Information
| Background | IL-8/CXCL8 |
|---|---|
| background_content | Background: IL-8/CXCL8 Interleukin 8 (IL-8), also named CXCL8, monocyte-derived neutrophil chemotactic factor (MDNCF), neutrophil-activating protein 1 (NAP-1), neutrophil-activating factor (NAF) and granulocyte chemotactic peptide (GCP), belongs to the Glu-Leu-Arg motif containing (ELR+) CXC chemokine family and has been designated CXCL8. IL-8 is a potent neutrophil chemoattractant that recruits neutrophils to sites of inflammation. IL-8 also activates neutrophil functions and promotes angiogenesis. The biological activites of IL-8 is mediated by two types of G protein-coupled chemokine receptors, CXCR1 and CXCR2 (1, 2). In normal tissues, IL-8 expression and secretion is barely detectable. Upon stimulation by a wide range of pro-inflammatory signals including exposure to IL-1, TNF, bacterial or viral products, IL-8 production is rapidly induced in many different cell types (3, 4). Feline IL-8 encodes a 101 amino acid (aa) precursor protein with a putative 22 aa signal peptide. It shares 61% and 76% aa sequence identity with human and canine IL-8, respectively. |
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