抗Human CXCR4抗体(Anti-Human CXCR4 antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	3T3 cells transfected with human CXCR4
Source	Monoclonal Mouse IgG2B Clone # 44716
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects human CXCR4. It will also react with cells expressing feline CXCR4 but not rat CXCR4.

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Applications and Data

	Recommended Concentration	Sample
Flow Cytometry	0.25 µg/106 cells	See below
Immunohistochemistry	8-25 µg/mL	See below
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Neutralization	Measured by its ability to neutralize CXCL12/SDF‑1 alpha -induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR4. The Neutralization Dose (ND50) is typically 2.5-12 µg/mL in the presence of 1 ng/mL Recombinant Human/Feline/Rhesus Macaque CXCL12/SDF‑1 alpha.

Flow Cytometry
	Detection of CXCR4 in Jurkat Human Cell Line by Flow Cytometry. Jurkat human acute T cell leukemia cell line was stained with Mouse Anti-Human CXCR4 Monoclonal Antibody (Catalog # MAB172, filled histogram) or isotype control antibody (Catalog # MAB004, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.
Immunohistochemistry
	CXCR4 in Human Lymph Node. CXCR4 was detected in immersion fixed paraffin-embedded sections of human lymph node using 15 µg/mL Human CXCR4 Monoclonal Antibody (Catalog # MAB172) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-AEC Cell & Tissue Staining Kit (red; Catalog # CTS003) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Immunohistochemistry
	CXCR4 in Human Spleen. CXCR4 was detected in immersion fixed paraffin-embedded sections of human spleen using Mouse Anti-Human CXCR4 Monoclonal Antibody (Catalog # MAB172) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Neutralization
	Chemotaxis Induced by CXCL12/SDF‑1 alpha and Neutralization by Human CXCR4 Antibody. Recombinant Human/Feline/Rhesus Macaque CXCL12/SDF‑1 alpha (Catalog # 350-NS) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR4 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human/Feline/Rhesus Macaque CXCL12/SDF‑1 alpha (1 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CXCR4 Monoclonal Antibody (Catalog # MAB172). The ND50 is typically 2.5-12 µg/mL.

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Related Product & Information

Entrez Gene IDs	7852 (Human); 12767 (Mouse); 60628 (Rat); 483900 (Canine); 493676 (Feline)
Background	CXCR4
background_content	Background: CXCR4 CXCR4 is a G-protein-linked seven transmembrane spanning receptor that binds stromal cell-derived factor-1 (SDF-1). CXCR4 acts as a co-factor for T-cell tropic HIV-1 and -2 viral entry into cells. While primarily a membrane protein, CXCR4 undergoes trafficking and internalization in response to stimulation with phorbol esters and ligand (1). Cytoplasmic and nuclear localization of CXCR4 has been observed in colorectal and renal carcinomas (2,3) and it has been used as the basis of prognosis and metastatic state (3,4,5).

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Citations

1. Delayed and repeated intranasal delivery of bone marrow stromal cells increases regeneration and functional recovery after ischemic stroke in mice
   Authors: MJ Chau, TC Deveau, X Gu, YS Kim, Y Xu, SP Yu, L Wei
   BMC Neurosci, 2018;19(1):20.
   Species: Rat
   Sample Type: Whole Cells
   Application: ICC


2. Mechanisms by which CXCR4/CXCL12 cause metastatic behavior in pancreatic cancer
   Authors: J Zhang, C Liu, X Mo, H Shi, S Li
   Oncol Lett, 2018;15(2):1771-1776.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC


3. Expression of CXC-motif-chemokine 12 and the receptor C-X-C receptor 4 in glioma and theeffect on peritumoral brain edema
   Authors: W Tang, Y Chen, X Wang, Y Chen, J Zhang, Z Lin
   Oncol Lett, 2018;15(2):2501-2507.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC-P


4. Cell-based therapy using miR-302-367 expressing cells represses glioblastoma growth
   Authors: M Fareh, F Almairac, L Turchi, F Burel-Vand, P Paquis, D Fontaine, S Lacas-Gerv, MP Junier, H Chneiweiss, T Virolle
   Cell Death Dis, 2017;8(3):e2713.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


5. The Glycosylphosphatidylinositol-Anchored Variable Region of Llama Heavy Chain-Only Antibody JM4 Efficiently Blocks both Cell-Free and T Cell-T Cell Transmission of Human Immunodeficiency Virus Type 1
   Authors: L Liu, W Wang, J Matz, C Ye, L Bracq, J Delon, JT Kimata, Z Chen, S Benichou, P Zhou
   J. Virol., 2016;90(23):10642-10659.
   Species: Human
   Sample Type: Whole Cells
   Application: Functional Assay


6. Cathepsin K cleavage of SDF-1? inhibits its chemotactic activity towards glioblastoma stem-like cells
   Authors: Vashendriya V V Hira
   Biochim. Biophys. Acta, 2016;0(0):.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


7. Simultaneous Activation of Induced Heterodimerization between CXCR4 Chemokine Receptor and Cannabinoid Receptor 2 (CB2) Reveals a Mechanism for Regulation of Tumor Progression
   Authors: CJ Coke, KA Scarlett, MA Chetram, KJ Jones, BJ Sandifer, AS Davis, AI Marcus, CV Hinton
   J. Biol. Chem., 2016;291(19):9991-10005.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


8. Signal transmission through the CXC chemokine receptor 4 (CXCR4) transmembrane helices
   Proc Natl Acad Sci USA, 2016;0(0):.
   Species: Human
   Sample Type: Whole Cells
   Application: IHC Fresh


9. The long pentraxin PTX3 promotes fibrocyte differentiation.
   Authors: Pilling D, Cox N, Vakil V, Verbeek J, Gomer R
   PLoS ONE, 2015;10(3):e0119709.
   Species: Human
   Sample Type: Whole Cells
   Application: IHC Fresh


10. Prevailing role of contact guidance in intrastromal T-cell trapping in human pancreatic cancer.
    Authors: Hartmann N, Giese N, Giese T, Poschke I, Offringa R, Werner J, Ryschich E
    Clin Cancer Res, 2014;20(13):3422-33.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC

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