抗IL-33抗体(Anti-IL-33, Rat-Mono, PE antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse
Label	Phycoerythrin
Immunogen	E. coli-derived recombinant mouse IL-33Ser109-Ile266Accession # Q8BVZ5
Source	Monoclonal Rat IgG2A Clone # 396118
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects mouse IL-33 in direct ELISAs.

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Applications and Data

	Recommended Concentration	Sample
Intracellular Staining by Flow Cytometry	10 µL/106 cells	See below

Intracellular Staining by Flow Cytometry

Detection of IL‑33 in bEnd.3 Mouse Cell Line by Flow Cytometry. bEnd.3 mouse endothelioma cell line was stained with Rat Anti‑Mouse IL‑33 PE‑conjugated Mono­clonal Antibody (Catalog # IC3626P, filled histogram) or isotype control antibody (Catalog # IC006P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

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Related Product & Information

Background

IL-33

background_content

Background: IL-33 IL-33, also known as NF-HEV and DVS 27, is a 30 kDa proinflammatory protein that may also regulate gene transcription (1-3). DVS 27 was identifed as a gene that is upregulated in vasospastic cerebral arteries (1). NF-HEV was described as a nuclear factor that is preferentially expressed in the endothelial cells of high endothelial venules relative to endothelial cells from other tissues (2). IL-33 was identified based on sequence and structural homology with IL-1 family cytokines (3). DVS 27, NF-HEV, and IL-33 share 100% amino acid sequence identity. IL-33 is constitutively expressed in smooth muscle and airway epithelia. It is upregulated in arterial smooth muscle, dermal fibroblasts, and keratinocytes following IL-1 alpha or IL-1 beta stimulation (1, 3). Similar to IL-1, IL-33 can be cleaved in vitro by caspase-1, generating an N-terminal fragment that is slightly shorter than the C-terminal fragment (3, 4). The N-terminal portion of full length IL-33 contains a predicted bipartite nuclear localization sequence and a homeodomain-like helix-turn-helix DNA binding domain. By immunofluorescence, full length IL-33 localizes to the nucleus in HUVECs and transfectants (2). The C-terminal fragment, corresponding to mature IL-33, binds and triggers signaling through mast cell IL-1 R4/ST2L, a longtime orphan receptor involved in the augmentation of Th2 cell responses (3, 5-7). A ternary signaling complex is formed by the subsequent association of IL-33 and ST2L with IL-1R AcP (8). Stimulation of Th2 polarized lymphocytes with mature IL-33 in vitro induces IL-5 and IL-13 secretion (3). In vivo administration of mature IL-33 promotes increased production of IL-5, IL-13, IgE, and IgA, as well as splenomegaly and inflammatory infiltration of mucosal tissues (3). Full length and mature mouse IL-33 share approximately 55% and 90% amino acid (aa) sequence identity with human and rat IL-33, respectively. Mouse IL-33 shares less than 25% aa sequence identity with other IL-1 family proteins.

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Citations

1. IL-33/ST2-mediated inflammation in macrophages is directly abrogated by IL-10 during rheumatoid arthritis
   Authors: S Chen, B Chen, Z Wen, Z Huang, L Ye
   Oncotarget, 2017;8(20):32407-32418.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


2. Dopaminergic Toxin 1-Methyl-4-Phenylpyridinium, Proteins alpha-Synuclein and Glia Maturation Factor Activate Mast Cells and Release Inflammatory Mediators.
   Authors: Kempuraj D, Thangavel R, Yang E, Pattani S, Zaheer S, Santillan D, Santillan M, Zaheer A
   PLoS ONE, 2015;10(8):e0135776.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


3. Type II cytokines impair host defense against an intracellular fungal pathogen by amplifying macrophage generation of IL-33.
   Authors: Verma, A, Kroetz, D N, Tweedle, J L, Deepe, G S Jr
   Mucosal Immunol, 2015;8(2):380-9.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


4. Murine mast cells secrete and respond to interleukin-33.
   Authors: Tung, Hui-Ying, Plunkett, Beverly, Huang, Shau-Ku, Zhou, Yufeng
   J Interferon Cytokine Res, 2014;34(3):141-7.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


5. IL-33 targeting attenuates intestinal mucositis and enhances effective tumor chemotherapy in mice.
   Authors: Guabiraba R, Besnard A, Menezes G, Secher T, Jabir M, Amaral S, Braun H, Lima-Junior R, Ribeiro R, Cunha F, Teixeira M, Beyaert R, Graham G, Liew F
   Mucosal Immunol, 2014;7(5):1079-93.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


6. Interleukin-33 and alveolar macrophages contribute to the mechanisms underlying the exacerbation of IgE-mediated airway inflammation and remodelling in mice.
   Authors: Mizutani N, Nabe T, Yoshino S
   Immunology, 2013;139(2):205-18.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


7. Trefoil factor 2 rapidly induces interleukin 33 to promote type 2 immunity during allergic asthma and hookworm infection.
   Authors: Wills-Karp M, Rani R, Dienger K
   J. Exp. Med., 2012;209(3):607-22.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow

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