抗IL-12/IL-35抗体(Anti-IL-12/IL-35, Mouse-Mono, PE antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human, Mouse
Label	Phycoerythrin
Immunogen	S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL‑12/IL-35 p35Arg23-Ser219Accession # P29459
Source	Monoclonal Mouse IgG1 Clone # 27537
Purification	Protein A or G purified from ascites
Specificity	Detects human IL‑12/IL-35 p35 in direct ELISAs and Western blots. Detects the p35 subunit either as part of a p40/p35 heterodimer or as a free subunit after reduction of the heterodimer. This antibody does not recognize IL-12 p40 homodimers but shows strong cross-reactivity with the p35 subunits from porcine and mouse systems.

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Applications and Data

	Recommended Concentration	Sample
Intracellular Staining by Flow Cytometry	10 µL/106 cells	See below

Intracellular Staining by Flow Cytometry
	Detection of IL‑12/IL‑35 p35 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) either untreated (orange open histogram) or treated with LPS (orange filled histogram) were stained with Mouse Anti-Human/Mouse IL‑12/IL‑35 p35 PE‑conjugated Monoclonal Antibody (Catalog # IC2191P) or isotype control antibody (Catalog # IC002P, blue open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Intracellular Staining by Flow Cytometry
	Detection of IL‑12/IL‑35 p35 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes either untreated (orange open histogram) or treated with LPS (orange filled histogram) were stained with Mouse Anti-Human/Mouse IL‑12/IL‑35 p35 PE‑conjugated Monoclonal Antibody (Catalog # IC2191P) or isotype control antibody (Catalog # IC002P, blue open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

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Related Product & Information

Background

IL-12/IL-35 p35

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Background: IL-12/IL-35 p35 Interleukin 12 (IL-12) and interleukin 35 (IL-35) are heterodimeric cytokines composed of alpha and beta chains. IL-12 is composed of p35 and p40 subunits, while IL-35 is comprised of p35 paired with EBI-3 (1). In mice, IL-35 is produced by FoxP3+ regulatory T cells and may function as an inhibitory cytokine to suppress T cell proliferation (2). Human FoxP3+ Tregs do not constitutively express IL-35 (3), but expression may be induced by activated dendritic cells (4).

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Citations

1. Reprogramming antitumor immunity against chemoresistant ovarian cancer by a CXCR4 antagonist-armed viral oncotherapy
   Authors: Marcin P Komorowski
   Mol Ther Oncolytics, 2016;3(0):16034.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


2. IL-35 over-expression increases apoptosis sensitivity and suppresses cell growth in human cancer cells.
   Biochem Biophys Res Commun, 2013;430(1):364-9.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


3. Generation of inducible immortalized dendritic cells with proper immune function in vitro and in vivo.
   Authors: Richter C, Thieme S, Bandola J, Laugsch M, Anastassiadis K, Brenner S
   PLoS ONE, 2013;8(4):e62621.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


4. Cutting edge: Human regulatory T cells require IL-35 to mediate suppression and infectious tolerance.
   Authors: Chaturvedi V, Collison LW, Guy CS, Workman CJ, Vignali DA
   J. Immunol., 2011;186(12):6661-6.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow

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