抗IL-12/IL-35 p35抗体(Anti-IL-12/IL-35 p35, Mouse-Mono, APC antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human, Mouse
Label	Allophycocyanin
Immunogen	S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL‑12/IL-35 p35Arg23-Ser219Accession # P29459
Source	Monoclonal Mouse IgG1 Clone # 27537
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects human IL‑12/IL-35 p35 in direct ELISAs and Western blots. Detects the p35 subunit either as part of a p40/p35 heterodimer or as a free subunit after reduction of the heterodimer. This antibody does not recognize IL-12 p40 homodimers but shows strong cross-reactivity with the p35 subunits from porcine and mouse systems.

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Applications and Data

	Recommended Concentration	Sample
Intracellular Staining by Flow Cytometry	10 µL/106 cells	See below

Intracellular Staining by Flow Cytometry

Detection of IL‑12/IL‑35 p35 in Human PBMCs or Mouse Splenocytes by Flow Cytometry. (A) Human peripheral blood mononuclear cell (PBMCs) or (B) mouse splenocytes either untreated (light orange filled histogram) or treated with LPS (dark orange filled histogram) were stained with Mouse Anti-Human/Mouse IL‑12/IL‑35 p35 APC‑conjugated Monoclonal Antibody (Catalog # IC2191A) or isotype control antibody (Catalog # IC002A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

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Related Product & Information

Long Name	Interleukin 12 p35
Background	IL-12/IL-35 p35
background_content	Background: IL-12/IL-35 p35 p35 is a 34-36 kDa monomeric member of the IL-6 superfamily of molecules. It is 219 amino acids in length, and possibly contains an N-terminal 22 aa signal sequence. In any event, it does not appear to be individually secreted, and its release from cells is commonly assumed to be as part of a disulfide-linked heterodimer linked to either p40 (forming IL-12) or EBI3 (forming IL-35). Multiple cell types are known to express p35, and include keratinocytes, CD138+ plasma cells, naïve B cells, monocyte-derived dendritic cells, macrophages, CD86lo B7-H1hi CD206+ B7-DC+ tolerogenic dendritic cells, neutrophils, activated monocytes and macrophages, trophoblasts, testicular endothelial cells, and T cells, including CD4+ and CD8+ T helper cells, and CD4+ CD25+ Tregs. The presence of the subunit does not necessarily indicate function, as the p35:p40 heterodimer is associated with promoting inflammatory IFN-gamma production by NK cells, while the p35:EBI3 heterodimer is anti-inflammatory through its inhibition of Th17 cell development. Over aa 23-219, human p35 shares 83% and 57% aa sequence identity with pig and mouse p35, respectively.

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Citations

1. Differential regulation of interleukin-12 (IL-12)/IL-23 by Tim-3 drives T(H)17 cell development during hepatitis C virus infection.
   Authors: Wang, Jia M, Shi, Lei, Ma, Cheng J, Ji, Xiao J, Ying, Ruo S, Wu, Xiao Y, Wang, Ke S, Li, Guangyu, Moorman, Jonathan, Yao, Zhi Q
   J Virol, 2013;87(8):4372-83.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


2. Regulatory T cells that recognize a ubiquitous stress-inducible self-antigen are long-lived suppressors of autoimmune arthritis.
   Authors: van Herwijnen M, Wieten L, van der Zee R, van Kooten P, Wagenaar-Hilbers J, Hoek A, den Braber I, Anderton S, Singh M, Meiring H, van Els C, van Eden W, Broere F
   Proc Natl Acad Sci U S A, 2012;109(35):14134-9.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow

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