抗Mouse IL-27 Fluorescein抗体(Anti-Mouse IL-27 Fluorescein antibody)
掲載日情報：2018/11/26 現在Webページ番号：195822
Mouse IL-27 Fluoresceinに対する抗体(Anti-Mouse IL-27 Fluorescein )です。
※ 本製品は研究用です。研究用以外には使用できません。
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価格
[在庫・価格 ：2024年05月31日 00時00分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
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Anti-Mouse IL-27 Fluorescein MAb (Clone 234205) |
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[在庫・価格 ：2024年05月31日 00時00分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
Anti-Mouse IL-27 Fluorescein MAb (Clone 234205)
文献数： 1
- 商品コード：IC1834F
- メーカー：RSD
- 包装：100tests
- 価格：¥66,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 |
別名：IL27 クローン：234205 Genbank No： 246778 Protein Accession No： O35228(EBI3) Q8K3I6(p28) |
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| 法規制等 | |||
| 保存条件 | 法規備考 | ||
| 抗原種 | Mouse | 免疫動物 | Rat |
| 交差性 | Mouse | 適用 | FCM |
| 標識 | FITC | 性状 | Protein A/G Affinity Purified |
| 吸収処理 | クラス | IgG | |
| クロナリティ | Monoclonal | フォーマット | |
| 掲載カタログ |
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| 製品記事 |
Helper T Cells Marker Antibodies |
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| 関連記事 | |||
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Product Details
| Species Reactivity | Mouse |
|---|---|
| Label | Fluorescein |
| Immunogen | Chinese hamster ovary cell line CHO-derived recombinant mouse IL‑27Tyr19-Pro228 (EBI3) & Phe29-Ser234 (p28)Accession # O35228 (EBI3) & Q8K3I6 (p28) |
| Source | Monoclonal Rat IgG2B Clone # 234205 |
| Purification | Protein A or G purified from hybridoma culture supernatant |
| Specificity | Detects mouse IL-27 in Western blots. This antibody recognizes the p28 subunit either as part of a p28/EBI-3 heterodimer or as a free subunit. |
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Applications and Data
| Recommended Concentration | Sample | |
| Intracellular Staining by Flow Cytometry | 10 µL/106 cells | See below |
| Intracellular Staining by Flow Cytometry | |
|---|---|
 | Detection of IL‑27 in RAW 264.7 Mouse Cell Line by Flow Cytometry. RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse IL‑27 Fluorescein‑conjugated Monoclonal Antibody (Catalog # IC1834F, filled histogram) or isotype control antibody (Catalog # IC013F, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules. |
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Related Product & Information
| Long Name | Interleukin 27 |
|---|---|
| Background | IL-27 |
| background_content | Background: IL-27 IL-27 is a non-covalent heterodimeric molecule that belongs to the IL-6/IL-12 family of long type I cytokines. It is composed of EBI3 (EBV-induced gene 3), a 33-34 kDa glycoprotein that is related to the p40 subunit of IL-12 and IL-23, and p28, the 28-30 kDa glycoprotein that is related to the p35 chain of IL-12. The mouse EBI3 gene encodes a 228 amino acid (aa) precursor that contains an 18 aa signal peptide and 210 aa mature protein. The mature region contains two potential N-linked glycosylation sites, two fibronectin type III domains, and two pairs of conserved cysteine residues with a WSXWS-like motif that places the molecule in the hematopoietin receptor family. Although p40, the EBI3 counterpart in IL-12, is known to form homodimers, there is no evidence to date that EBI3 also homodimerizes. EBI3 is known to heterodimerize with the IL-12 p35 subunit to form IL-35. Mature mouse EBI3 shares 91% and 61% aa sequence identity to mature rat and human EBI3, respectfully. The mouse p28 gene encodes a 234 aa precursor that contains a 28 aa signal sequence and 206 aa mature region. The mature region is characterized by the presence of four alpha -helices, placing it in the IL-6 family of helical cytokines. Mature mouse p28 shares 89% and 70% aa sequence identity with mature rat and human p28, respectfully. IL-27 is expressed by monocytes, endothelial cells and dendritic cells. IL-27 binds to and signals through a heterodimeric receptor complex composed of WSX-1 (TCCR) and gp130. Evidence suggests IL-27 interacts only with WSX-1. In addition, and in mouse but not human, p28 is secreted as a monomer and appears to activate the IL-6R complex. This is consistent with the observation that p28 also heterodimerizes with CLF1, and signals through the IL-6R:gp130 complex. IL-27 has both anti- and proinflammatory properties. As an anti‑inflammatory, IL-27 seems to induce a general negative feedback program that limits T and NK-T cell activity. At the onset of infection, IL-27 induces an IL‑12 receptor on naïve CD4+ T cells, making them susceptible to subsequent IL-12 activity that generates Th1 cells at the expense of Th2 and Th17 cells. Finally, IL-27 upregulates both MHC-II and chemokine (CXCL9; CXCL10) expression on vascular endothelium, suggesting a role for IL-27 in vascular inflammation. |
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Citations
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
- The transcription factor IRF8 activates integrin-mediated TGF-beta signaling and promotes neuroinflammation.
Authors: Yoshida Y, Yoshimi R, Yoshii H, Kim D, Dey A, Xiong H, Munasinghe J, Yazawa I, O'Donovan M, Maximova O, Sharma S, Zhu J, Wang H, Morse H, Ozato K
Immunity, 2014;40(2):187-98.
Species: Mouse
Sample Type: Whole Cells
Application: Flow
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