抗Human CD4 Alexa Fluor 488抗体(Anti-Human CD4 Alexa Fluor 488 antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Alexa Fluor 488
Immunogen	S. frugiperda insect ovarian cell line Sf 21-derived recombinant human CD4Lys26-Trp390Accession # P01730
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects human CD4 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse CD4, recombinant feline CD4, recombinant cotton rat CD4, and recombinant canine CD4 is observed.

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Applications and Data

	Recommended Concentration	Sample
Flow Cytometry	5 µL/106 cells	See below
Immunocytochemistry	8-25 µg/mL	See below

Flow Cytometry
	Detection of CD4 in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Goat Anti-Human CD4 Alexa Fluor® 488‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB8165G, filled histogram) or isotype control antibody (Catalog # IC108G, open histogram). View our protocol for Staining Membrane-associated Proteins.
Immunocytochemistry
	CD4 in Human PBMCs. CD4 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Goat Anti-Human CD4 Alexa Fluor® 488-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB8165G, green) at 10 µg/mL for 3 hours at room temperature. Cells were counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

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Related Product & Information

Background

CD4

background_content

Background: CD4 CD4 is an approximately 55 kDa type I membrane glycoprotein that is expressed predominantly on most thymocytes and a subset of mature T lymphocytes. In humans, CD4 is also expressed to a lesser extent on monocytes and macrophage related cells. Human CD4 cDNA encodes a 458 amino acid (aa) residue precursor protein with a 25 aa residue signal peptide, a 371 aa residue extracellular region containing four immunoglobulin homology domains, a 24 aa residue transmembrane domain and a 38 aa residue cytoplasmic domain. CD4 is a coreceptor required for T cell recognition of antigens that are presented by class II major histocompatibility complexes. CD4 has been shown to be a coreceptor of HIV entry and specifically binds gp120, the external envelope glycoprotein of HIV.

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Citations

1. Accumulation of follicular CD8+ T cells in pathogenic SIV infection
   Authors: S Ferrando-M, E Moysi, A Pegu, S Andrews, K Nganou Mak, D Ambrozak, AB McDermott, D Palesch, M Paiardini, GN Pavlakis, JM Brenchley, D Douek, JR Mascola, C Petrovas, RA Koup
   J. Clin. Invest., 2018;0(0):.
   Species: Primate
   Sample Type: Whole Cells
   Application: ICC


2. Treatment with native heterodimeric IL-15 increases cytotoxic lymphocytes and reduces SHIV RNA in lymph nodes
   Authors: DC Watson, E Moysi, A Valentin, C Bergamasch, S Devasundar, SP Fortis, J Bear, E Chertova, J Bess, R Sowder, DJ Venzon, C Deleage, JD Estes, JD Lifson, C Petrovas, BK Felber, GN Pavlakis
   PLoS Pathog., 2018;14(2):e1006902.
   Species: Macaca mulatta (Rhesus Macque)
   Sample Type: Whole Tissue
   Application: Confocal Imaging


3. Quantitative Multiplexed Imaging Analysis Reveals a Strong Association between Immunogen-Specific B Cell Responses and Tonsillar Germinal Center Immune Dynamics in Children after Influenza Vaccination
   Authors: D Amodio, N Cotugno, G Macchiarul, S Rocca, Y Dimopoulos, MR Castrucci, R De Vito, FM Tucci, AB McDermott, S Narpala, P Rossi, RA Koup, P Palma, C Petrovas
   J. Immunol., 2017;0(0):.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC

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