抗Mouse/Rat CCR2 Allophycocyanin抗体(Anti-Mouse/Rat CCR2 Allophycocyanin antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse
Label	Allophycocyanin
Immunogen	L1.2 mouse pro-B cell line transfected with mouse CCR2
Source	Monoclonal Rat IgG2B Clone # 475301
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects mouse CCR2 transfectants but not the parental cell line in flow cytometry. Does not detect HEK293 human embryonic kidney cell line transfected with mouse CCR5 in flow cytometry.

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Applications and Data

	Recommended Concentration	Sample
Flow Cytometry	10 µL/106 cells	See below

Flow Cytometry
	Detection of CCR2 in L1.2 Mouse Cell Line Transfected with Mouse CCR2 by Flow Cytometry. L1.2 mouse pro-B cell line transfected with mouse CCR2 was stained with Rat Anti-Mouse CCR2 APC‑conjugated Monoclonal Antibody (Catalog # FAB5538A, filled histogram) or isotype control antibody (Catalog # IC013A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Flow Cytometry
	Detection of CCR2 in Mouse Splenocytes byFlow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse CCR2 APC‑conjugated Monoclonal Antibody (Catalog # FAB5538A) and Rat Anti-Mouse Gr‑1/Ly‑6G Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB1037F). View our protocol for Staining Membrane-associated Proteins.

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Related Product & Information

Entrez Gene IDs	729230 (Human); 12772 (Mouse); 60463 (Rat)
Background	CCR2
background_content	Background: CCR2 CCR2, also known as CD192, is a 38 kDa 7TM chemokine receptor that preferentially binds CCL2, CCL7, and CCL13. CCR2 is expressed by multiple hematopoietic cells, endothelial cells, fibroblasts, neurons, and smooth muscle cells. It functions as an HIV fusion co-factor and facilitates T cell recruitment during inflammation. Two alternate splice forms (CCR2A and CCR2B) differ only by the addition of 14 amino acids to the intracellular carboxyl terminal. Mouse CCR2 shares 67% and 95% aa sequence identity with human and rat CCR2, respectively.

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Citations

1. Effect of Anti-C5a Therapy in a Murine Model of Early/Intermediate Dry Age-Related Macular Degeneration
   Authors: CB Toomey, M Landowski, M Klingeborn, U Kelly, J Deans, H Dong, O Harrabi, T Van Blarco, YA Yeung, R Grishanin, JC Lin, DR Saban, C Bowes Rick
   Invest. Ophthalmol. Vis. Sci., 2018;59(2):662-673.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


2. Myeloid cells that impair immunotherapy are restored in melanomas which acquire resistance to BRAF inhibitors
   Authors: SM Steinberg, T Shabaneh, P Zhang, V Martyanov, Z Li, B Malik, T Wood, A Boni, A Molodtsov, CV Angeles, TJ Curiel, M Whitfield, MJ Turk
   Cancer Res, 2017;0(0):.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


3. CCR2(-) and CCR2(+) corneal macrophages exhibit distinct characteristics and balance inflammatory responses after epithelial abrasion
   Authors: J Liu, Y Xue, D Dong, C Xiao, C Lin, H Wang, F Song, T Fu, Z Wang, J Chen, H Pan, Y Li, D Cai, Z Li
   Mucosal Immunol, 2017;0(0):.
   


4. Impact of myeloid-derived suppressor cell on Kupffer cells from mouse livers with hepatocellular carcinoma
   Authors: Stéphanie Lacotte
   Oncoimmunology, 2016;5(11):e1234565.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


5. Pivotal role for skin trans-endothelial radio-resistant anti-inflammatory macrophages in tissue repair
   Authors: Olga Barreiro
   Elife, 2016;5(0):.
   Species: Mouse
   Sample Type: Tissue Homogenates
   Application: Flow


6. Alternatively activated macrophages determine repair of the infarcted adult murine heart
   J Clin Invest, 2016;0(0):.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


7. Monocyte Trafficking, Engraftment, and Delivery of Nanoparticles and an Exogenous Gene into the Acutely Inflamed Brain Tissue - Evaluations on Monocyte-Based Delivery System for the Central Nervous System
   PLoS ONE, 2016;11(4):e0154022.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


8. Vitamin D deficiency decreases survival of bacterial meningoencephalitis in mice.
   Authors: Djukic M, Sostmann N, Bertsch T, Mecke M, Nessler S, Manig A, Hanisch U, Triebel J, Bollheimer L, Sieber C, Nau R
   J Neuroinflammation, 2015;12(0):208.
   Species: Mouse
   Sample Type: Tissue Homogenates
   Application: Flow


9. Donor colonic CD103+ dendritic cells determine the severity of acute graft-versus-host disease.
   Authors: Koyama M, Cheong M, Markey K, Gartlan K, Kuns R, Locke K, Lineburg K, Teal B, Leveque-El Mouttie L, Bunting M, Vuckovic S, Zhang P, Teng M, Varelias A, Tey S, Wockner L, Engwerda C, Smyth M, Belz G, McColl S, MacDonald K, Hill G
   J Exp Med, 2015;212(8):1303-21.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


10. SHP-1-dependent macrophage differentiation exacerbates virus-induced myositis.
    Authors: Watson N, Schneider K, Massa P
    J Immunol, 2015;194(6):2796-809.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow

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