抗Human BMP-2 Biotinylated抗体(Anti-Human BMP-2 Biotinylated antibody)
掲載日情報：2021/01/28 現在Webページ番号：195227
Human BMP-2 Biotinylatedに対する抗体(Anti-Human BMP-2 Biotinylated )です。
※ 本製品は研究用です。研究用以外には使用できません。
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- 価格
- Product Details
- Applications and Data
- References
- Related Research Areas
- Related Product & Information
価格
[在庫・価格 ：2025年04月26日 20時35分現在]
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Anti-Human BMP-2 Biotinylated MAb (Clone 100230) |
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[在庫・価格 ：2025年04月26日 20時35分現在]
Anti-Human BMP-2 Biotinylated MAb (Clone 100230)
文献数： 0
- 商品コード：BAM3552
- メーカー：RSD
- 包装：250μg
- 価格：¥118,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | マッチドペア：Human BMP-2 サンドイッチELISAの検出用抗体として利用可能，補足用抗体として#MAB3551-500，スタンダードとして#355-BM-010を用いる。 別名：BMP2 クローン：100230 Genbank No： 650 Protein Accession No： P12643 |
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| 保存条件 | -20℃ | 法規備考 | |
| 抗原種 | 免疫動物 | Mouse | |
| 交差性 | Human | 適用 | ELISA |
| 標識 | Biotin | 性状 | Protein A/G Affinity Purified |
| 吸収処理 | クラス | IgG | |
| クロナリティ | Monoclonal | フォーマット | |
| 掲載カタログ |
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| 製品記事 | |||
| 関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Product Details
| Species Reactivity | Human |
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| Label | Biotin |
| Immunogen | Chinese hamster ovary cell line CHO-derived recombinant human BMP-2Gln283-Arg396Accession # P12643 |
| Source | Monoclonal Mouse IgG1 Clone # 100230 |
| Purification | Protein A or G purified from hybridoma culture supernatant |
| Specificity | Detects human BMP-2 in ELISAs. In sandwich immunoassays, less than 10% cross-reactivity with recombinant human (rh) BMP‑4, rhBMP‑2/BMP‑7 heterodimer, and no cross-reactivity with rhBMP-3, -5, -6, -7, rhTGF-beta 1, rhTGF-beta 2, or rhTGF-beta 3 is observed. |
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Applications and Data
| Recommended Concentration | Sample | |
| Human BMP-2 Sandwich Immunoassay | Reagent | |
| ELISA Capture (Matched Antibody Pair) | 2-8 µg/mL | Human BMP‑2 Antibody (Catalog #MAB3551 ) |
| ELISA Detection (Matched Antibody Pair) | 0.5-2.0 µg/mL | Human BMP‑2 Biotinylated Antibody (Catalog #BAM3552 ) |
| ELISA Standard | Recombinant Human/Mouse/Rat BMP-2 Protein (Catalog #355-BM ) | |
| Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website. | Preparation and Storage | |
| Reconstitution | Reconstitute at 0.5 mg/mL in sterile PBS. | Reconstitution Buffer Available |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. | |
| 1 month, 2 to 8 °C under sterile conditions after reconstitution. | ||
| 6 months, -20 to -70 °C under sterile conditions after reconstitution. | ||
| Background: BMP-2 | Bone morphogenetic protein 2 (BMP-2) is a member of the BMP subgroup of the TGF-beta superfamily. It plays a dominant role in embryonic dorsal-ventral patterning, organogenesis, limb bud formation, and bone formation and regeneration (1, 2). Human BMP-2 is synthesized as a 396 amino acid (aa) preproprotein that contains a 23 aa signal sequence, a 259 aa prosegment, and a 114 aa mature region (3). Proteolytic removal of the propeptide enables mature BMP-2 to form active disulfide linked homodimers and heterodimers with BMP-7 (2). Mature monomeric BMP-2 is an 18 kDa glycosylated peptide with seven conserved cysteines that form a cystine knot structure (4). Mature human BMP-2 shares 100% aa sequence identity with mouse and rat BMP-2. It shares 85% aa sequence identity with human BMP-4 and less than 51% with other BMPs. BMP-2 signals through heterodimeric complexes composed of a type I receptor (Activin RI, BMPR-IA, or BMPR-IB) and a type II receptor (BMP RII or Activin RIIB) (2, 5). BMP-2 induces chondrocyte proliferation, endochondral bone formation, longitudinal bone growth, and bone and cartilage repair (6, 7). It induces ectopic bone formation or calcification by promoting osteogenic and chondrogenic differentiation in mesenchymal cells, stem cells, and vascular smooth muscle cells (2, 8‑10). BMP-2/BMP-7 heterodimers are significantly more potent than BMP-2 homodimers at inducing bone formation in vivo (11). BMP-2 also promotes the maintenance and repair of colonic epithelium, suppresses neuronal dopamine synthesis and release, induces apoptosis in medulloblastoma cells, and is required for cardiac contractility (12‑15). |
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References
| Kishigami, S. and Y. Mishina (2005) Cytokine Growth Factor Rev. 16:265. | |
| Chen, D. et al. (2004) Growth Factors 22:233. | |
| Wozney, J. et al. (1988) Science 242:1528. | |
| Sun, P.D. and D.R. Davies (1995) Annu. Rev. Biophys. Biomol. Struct. 24:269. | |
| Sebald, W. et al. (2004) Biol. Chem. 385:697. | |
| De Luca, F. et al. (2001) Endocrinology 142:430. | |
| Davidson, E.N.B., et al. (2007) Arthritis Res. Ther. 9:R102. | |
| Ryoo, H.-M. et al. (2006) Gene 366:51. | |
| Kramer, J. et al. (2000) Mech. Dev. 92:193. | |
| Li, X. et al. (2008) Atherosclerosis January 5 epub. | |
| Zhu, W. et al. (2004) J. Bone Miner. Res. 19:2021. | |
| Peiris, D. et al. (2007) Am. J. Physiol. Gastrointest. Liver Physiol. 292:G753. | |
| Kano, Y. et al. (2005) Endocrinology 146:5332. | |
| Hallahan, A.R. et al. (2003) Nat. Med. 9:1033. | |
| Wang, Y.-X. et al. (2007) Cardiovasc. Res. 74:290. | |
| Long Name: | Bone Morphogenetic Protein 2 |
| Entrez Gene IDs: | 650 (Human); 12156 (Mouse); 29373 (Rat) |
| Alternate Names: | BMP2; BMP-2; BMP-2A; BMP2ABone morphogenetic protein 2A; bone morphogenetic protein 2 |
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Related Research Areas
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Related Product & Information
| Background | BMP-2 |
|---|---|
| background_content | Background: BMP-2 Bone morphogenetic protein 2 (BMP-2) is a member of the BMP subgroup of the TGF-beta superfamily. It plays a dominant role in embryonic dorsal-ventral patterning, organogenesis, limb bud formation, and bone formation and regeneration (1, 2). Human BMP-2 is synthesized as a 396 amino acid (aa) preproprotein that contains a 23 aa signal sequence, a 259 aa prosegment, and a 114 aa mature region (3). Proteolytic removal of the propeptide enables mature BMP-2 to form active disulfide linked homodimers and heterodimers with BMP-7 (2). Mature monomeric BMP-2 is an 18 kDa glycosylated peptide with seven conserved cysteines that form a cystine knot structure (4). Mature human BMP-2 shares 100% aa sequence identity with mouse and rat BMP-2. It shares 85% aa sequence identity with human BMP-4 and less than 51% with other BMPs. BMP-2 signals through heterodimeric complexes composed of a type I receptor (Activin RI, BMPR-IA, or BMPR-IB) and a type II receptor (BMP RII or Activin RIIB) (2, 5). BMP-2 induces chondrocyte proliferation, endochondral bone formation, longitudinal bone growth, and bone and cartilage repair (6, 7). It induces ectopic bone formation or calcification by promoting osteogenic and chondrogenic differentiation in mesenchymal cells, stem cells, and vascular smooth muscle cells (2, 8‑10). BMP-2/BMP-7 heterodimers are significantly more potent than BMP-2 homodimers at inducing bone formation in vivo (11). BMP-2 also promotes the maintenance and repair of colonic epithelium, suppresses neuronal dopamine synthesis and release, induces apoptosis in medulloblastoma cells, and is required for cardiac contractility (12‑15). |
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