抗Mouse Lipocalin-2/NGAL Biotinylated抗体(Anti-Mouse Lipocalin-2/NGAL Biotinylated antibody)
掲載日情報:2021/01/28 現在Webページ番号:195209
Mouse Lipocalin-2/NGAL Biotinylatedに対する抗体(Anti-Mouse Lipocalin-2/NGAL Biotinylated )です。
※ 本製品は研究用です。研究用以外には使用できません。
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- 価格
- Product Details
- Applications and Data
- References
- Related Research Areas
- Related Product & Information
価格
[在庫・価格 :2024年05月18日 00時00分現在]
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Anti-Mouse Lipocalin-2/NGAL Biotinylated MAb (Clone 228418) |
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[在庫・価格 :2024年05月18日 00時00分現在]
Anti-Mouse Lipocalin-2/NGAL Biotinylated MAb (Clone 228418)
文献数: 0
- 商品コード:BAM1857
- メーカー:RSD
- 包装:250μg
- 価格:¥118,000
- 在庫:無(未発注)
- 納期:10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等:
説明文 |
マッチドペア:Mouse Lipocalin-2/NGAL サンドイッチELISAの検出用抗体として利用可能,補足用抗体として#MAB18571-500,スタンダードとして#1857-LC-050を用いる。 別名:24p3 クローン:228418 Genbank No: 3934 Protein Accession No: P11672 |
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法規制等 | |||
保存条件 | 法規備考 | ||
抗原種 | 免疫動物 | Rat | |
交差性 | Mouse | 適用 | ELISA |
標識 | Biotin | 性状 | Protein A/G Affinity Purified |
吸収処理 | クラス | IgG | |
クロナリティ | Monoclonal | フォーマット | |
掲載カタログ |
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製品記事 | |||
関連記事 |
R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Product Details
Species Reactivity | Mouse |
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Label | Biotin |
Immunogen | Mouse myeloma cell line NS0-derived recombinant mouse Lipocalin-2/NGALGln21-Asn200Accession # P11672 |
Source | Monoclonal Rat IgG2A Clone # 228418 |
Purification | Protein A or G purified from hybridoma culture supernatant |
Specificity | Detects mouse Lipocalin-2/NGAL in direct ELISAs. In sandwich immunoassays, no cross-reactivity or interference with recombinant human (rh) Lipocalin-1, rhLipocalin-2, or recombinant rat Lipocalin-2 is observed. |
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Applications and Data
Recommended Concentration | Sample | |
Mouse Lipocalin-2/NGAL Sandwich Immunoassay | Reagent | |
ELISA Capture (Matched Antibody Pair) | 2-8 µg/mL | Mouse Lipocalin‑2/NGAL Antibody (Catalog #MAB18571 ) |
ELISA Detection (Matched Antibody Pair) | 0.5-2.0 µg/mL | Mouse Lipocalin‑2/NGAL Biotinylated Antibody (Catalog #BAM1857 ) |
ELISA Standard | Recombinant Mouse Lipocalin-2/NGAL Protein, CF (Catalog #1857-LC ) | |
Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website. | Preparation and Storage | |
Reconstitution | Reconstitute at 0.5 mg/mL in sterile PBS. | Reconstitution Buffer Available |
Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | |
Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. | |
1 month, 2 to 8 °C under sterile conditions after reconstitution. | ||
6 months, -20 to -70 °C under sterile conditions after reconstitution. | ||
Background: Lipocalin-2/NGAL | Mouse Lipocalin-2 was cloned from mouse kidney cells (1). Its very high level of expression at the post-stratum uterus gave it the name uterocalin (2). Lipocalin-2 has been implicated in a variety of processes including cell differentiation, tumorigenesis, and apoptosis (3‑5). Studies indicate that Lipocalin-2 binds a bacterial catecholate siderophore that is bound to a ferric ion, such as enterobactin, with a subnanomolar dissociation constant (KD = 0.41 nM) (6). The bound ferric enterobactin complex breaks down slowly in a month into dihydroxybenzoyl serine and dihydroxybenzoic acid (DHBA). It also binds to a ferric DHBA complex with much less KD values (7.9 nM) (6). Secretion of Lipocalin-2 in immune cells increases in response to stimulation of Toll-like receptor as an acute phase response to infection. As a result, it acts as a potent bacteriostatic reagent by sequestering iron (7). Moreover, Lipocalin-2 can alter the invasive and metastatic behavior of Ras-transformed breast cancer cells in vitro and in vivo by reversing the epithelial to mesenchymal transition inducing activity of Ras, through restoration of E-cadherin expression, via effects on the Ras-MAPK signaling pathway (8). |
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References
Hraba-Renevey, s. et al. (1989) Oncogene. 4:601. | |
Liu, Q. et al. (1993) Mol Reprod Dev. 46:507. | |
Kjeldsen L, et al. (2000) Biochim Biophys Acta. 1482:272. | |
Devireddy, L.R. et al. (2001) Science 293:829. | |
Yang, M.B. et al. (2002) Mol. Cell. 10:1045. | |
Goetz, D.H. et al. (2002) Mol. Cell 10:1033. | |
Flo, T.H. et al. (2004) Nature 432:917. | |
Hanai, J. et al. (2005) J. Biol. Chem. 280:13641. | |
Long Name: | Neutrophil Gelatinase-associated Lipocalin |
Entrez Gene IDs: | 3934 (Human); 16819 (Mouse); 170496 (Rat); 102137149 (Cynomolgus Monkey) |
Alternate Names: | 24p3; 25 kDa alpha-2-microglobulin-related subunit of MMP-9; HNL; LCN2; lipocalin 2 (oncogene 24p3); lipocalin 2; Lipocalin2; Lipocalin-2; migration-stimulating factor inhibitor; MSFI; neutrophil gelatinase-associated lipocalin; NGAL; NGALlipocalin-2; Oncogene 24p3; p25; Siderocalin |
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Related Research Areas
Adipocytokines |
Cancer Biomarkers |
Metalloproteases and Regulators |
MMPs, TIMPs and Related Molecules |
Renal Markers |
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Related Product & Information
Background | Lipocalin-2/NGAL |
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background_content | Background: Lipocalin-2/NGAL Mouse Lipocalin-2 was cloned from mouse kidney cells (1). Its very high level of expression at the post-stratum uterus gave it the name uterocalin (2). Lipocalin-2 has been implicated in a variety of processes including cell differentiation, tumorigenesis, and apoptosis (3‑5). Studies indicate that Lipocalin-2 binds a bacterial catecholate siderophore that is bound to a ferric ion, such as enterobactin, with a subnanomolar dissociation constant (KD = 0.41 nM) (6). The bound ferric enterobactin complex breaks down slowly in a month into dihydroxybenzoyl serine and dihydroxybenzoic acid (DHBA). It also binds to a ferric DHBA complex with much less KD values (7.9 nM) (6). Secretion of Lipocalin-2 in immune cells increases in response to stimulation of Toll-like receptor as an acute phase response to infection. As a result, it acts as a potent bacteriostatic reagent by sequestering iron (7). Moreover, Lipocalin-2 can alter the invasive and metastatic behavior of Ras-transformed breast cancer cells in vitro and in vivo by reversing the epithelial to mesenchymal transition inducing activity of Ras, through restoration of E-cadherin expression, via effects on the Ras-MAPK signaling pathway (8). |
追加しました。
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