抗Human SOST Biotinylated抗体(Anti-Human SOST Biotinylated antibody)

• 価格
• Product Details
• Applications and Data
• References
• Related Research Areas
• Related Product & Information

価格

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Product Details

Species Reactivity	Human
Label	Biotin
Immunogen	Mouse myeloma cell line NS0-derived recombinant human SOST/SclerostinGln24-Tyr213Accession # Q9BQB4
Source	Monoclonal Mouse IgG1 Clone # 220910
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects human SOST/Sclerostin in ELISAs. In sandwich immunoassays, 30% cross-reactivity with recombinant mouse SOST is observed and no cross-reactivity or interference with recombinant human BMP-2, -4, -5, -6, or -7 was observed.

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Applications and Data

	Recommended Concentration	Sample
Human SOST Sandwich Immunoassay	Reagent
ELISA Capture (Matched Antibody Pair)	2-8 µg/mL	Human SOST/Sclerostin Antibody (Catalog #MAB1406 )
ELISA Detection (Matched Antibody Pair)	0.5-2.0 µg/mL	Human SOST/Sclerostin Biotinylated Antibody (Catalog #BAM14061 )
ELISA Standard		Recombinant Human SOST/Sclerostin Protein (Catalog #1406-ST )
Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website.		Preparation and Storage
Reconstitution	Reconstitute at 0.5 mg/mL in sterile PBS.	Reconstitution Buffer Available
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: SOST/Sclerostin	SOST, also known as sclerostin, is a member of the cerberus/DAN family, a group of secreted glycoproteins characterized by a cysteine-knot motif. Cerberus/DAN family members are putative BMP antagonists, and include Dan, Cerberus, Gremlin, PRDC, and Caronte. While the overall sequence identity between members of the family is low, they have conserved spacing of six cysteine residues. Cerberus and dan have an additional cysteine residue used for dimerization; however, SOST does not and is secreted as a monomer. SOST was originally identified as an important regulator of bone homesotasis. Positional cloning studies identified that mutations in the SOST gene can cause sclerosteosis and van Buchem disease, bone dysplasia disorders characterized by progressive skeletal overgrowth. Significant levels of SOST expression are detected in bone, cartilage, kidney, and liver. SOST is expressed by osteoclasts in developing bones of mouse embryos, including both intramembranously forming skull bones and endochondrally forming long bones. SOST plays a physiological role as a negative regulator of bone formation by repressing BMP-induced osteogenesis. SOST has been shown to have unique ligand specificity, binding BMP-5, -6, and -7 with high affinity and BMP-2 and -4 with low affinity. This seems to be the first example of a BMP antagonist being localized to osteoclasts, cells derived from the hematopoietic lineage, that function to degrade bone matrix. Recombinant human SOST preparations from R&D Systems bind BMP-5 and BMP-6 in a functional ELISA. Human and mouse SOST share 88% amino acid identity (1‑3).

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References

Kusu, N. et al. (2003) J. Biol. Chem. 278:24113.
Balemans, W. et al. (2001) Hum. Mol. Genet. 10:537.
Brunkow, M.E. et al. (2001) Am. J. Hum. Genet. 68:577.
Entrez Gene IDs:	50964 (Human); 74499 (Mouse)
Alternate Names:	sclerostin; SOST; VBCHsclerosteosis

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Related Research Areas

BMP Family

Osteocyte Markers

Osteogenesis Markers

TGF-beta Superfamily Modulators

Wnt Inhibitors

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Related Product & Information

Background

SOST/Sclerostin

background_content

Background: SOST/Sclerostin SOST, also known as sclerostin, is a member of the cerberus/DAN family, a group of secreted glycoproteins characterized by a cysteine-knot motif. Cerberus/DAN family members are putative BMP antagonists, and include Dan, Cerberus, Gremlin, PRDC, and Caronte. While the overall sequence identity between members of the family is low, they have conserved spacing of six cysteine residues. Cerberus and dan have an additional cysteine residue used for dimerization; however, SOST does not and is secreted as a monomer. SOST was originally identified as an important regulator of bone homesotasis. Positional cloning studies identified that mutations in the SOST gene can cause sclerosteosis and van Buchem disease, bone dysplasia disorders characterized by progressive skeletal overgrowth. Significant levels of SOST expression are detected in bone, cartilage, kidney, and liver. SOST is expressed by osteoclasts in developing bones of mouse embryos, including both intramembranously forming skull bones and endochondrally forming long bones. SOST plays a physiological role as a negative regulator of bone formation by repressing BMP-induced osteogenesis. SOST has been shown to have unique ligand specificity, binding BMP-5, -6, and -7 with high affinity and BMP-2 and -4 with low affinity. This seems to be the first example of a BMP antagonist being localized to osteoclasts, cells derived from the hematopoietic lineage, that function to degrade bone matrix. Recombinant human SOST preparations from R&D Systems bind BMP-5 and BMP-6 in a functional ELISA. Human and mouse SOST share 88% amino acid identity (1‑3).

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