抗TNF Receptor Ⅰ抗体(Anti-TNF Receptor Ⅰ, Soluble, Mouse, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

目次に戻る

Product Details

Species Reactivity	Mouse
Label	Unconjugated
Immunogen	E. coli-derived recombinant mouse TNF RIIle22-Ala212Accession # P25118
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects mouse TNF RI/TNFRSF1A in direct ELISAs and Western blots. In direct ELISAs and Western blots (non-reducing conditions), less than 5% cross-reactivity with recombinant human TNF RI is observed.

目次に戻る

Applications and Data

	Recommended Concentration	Sample
Western Blot	0.2 µg/mL	See below
Flow Cytometry	2.5 µg/106 cells	See below
Immunohistochemistry	5-15 µg/mL	Perfusion fixed frozen sections of mouse intestine
Agonist Activity	Measured in a cytotoxicity assay using L-929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 0.05-0.15 μg/mL.
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Western Blot
	Detection of Mouse TNF RI/TNFRSF1A by Western Blot. Western blot shows lysates of L‑929 mouse fibroblast cell line. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Mouse TNF RI/TNFRSF1A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-425-PB) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for TNF RI/TNFRSF1A at approximately 50-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Flow Cytometry
	Detection of TNF RI/TNFRSF1A in L‑929 Mouse Cell Line by Flow Cytometry. L‑929 mouse fibroblast cell line was stained with Goat Anti‑Mouse TNF RI/TNFRSF1A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-425-PB, filled histo­gram) or control antibody (Catalog # AB-108-C, open histo­gram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).

目次に戻る

Related Product & Information

Long Name	Tumor Necrosis Factor Receptor I
Entrez Gene IDs	7132 (Human); 21937 (Mouse); 25625 (Rat); 102135285 (Cynomolgus Monkey)
Background	TNF RI/TNFRSF1A
background_content	Background: TNF RI/TNFRSF1A TNF receptor 1 (TNF RI; also called TNF R‑p55/p60, TNFRSF1A and CD120a) is a type I transmembrane protein that belongs to the TNF receptor superfamily (1, 2). TNF RI is widely expressed and is present on the cell surface as a trimer of 55 kDa subunits. It serves as a receptor for both TNF‑ alpha and TNF‑ beta /lymphotoxin. Each subunit contains four TNF‑ alpha trimer‑binding cysteine‑rich domains (CRD) in its extracellular domain (ECD) (1‑6). TNF‑ alpha binding to TNF R1 induces the sequestration of TNFRI in lipid rafts, where it activates NF kappa B and is cleaved by ADAM‑17/TACE (7, 8). Release of the 28‑34 kDa TNF RI ECD occurs constitutively, and in response to products of pathogens such as LPS, CpG DNA or S. aureus protein A (1, 7‑12). Full‑length TNF RI may also be released in exosome‑like vesicles (12). Such release helps to resolve inflammatory reactions as it down‑regulates cell surface TNF RI and provides soluble TNF RI to bind TNF‑ alpha (6, 13, 14). Exclusion from lipid rafts causes endocytosis of TNF RI complexes and induces apoptosis (7, 15). Although there is a second receptor for TNF‑ alpha (TNF R2), TNF RI is thought to mediate most of the cellular effects of TNF‑ alpha (3). TNF R1 is essential for proper development of lymph node germinal centers and Peyer’s patches, and for combating intracellular pathogens such as Listeria monocytogenes (1‑3). Mouse TNF RI is a 454 amino acid (aa) protein that contains a 21 aa signal sequence and a 191 aa ECD with a PLAD domain (6). This mediates constitutive trimer formation. The PLAD domain is followed by four CRDs, a 23 aa transmembrane domain, and a 219 aa cytoplasmic sequence that contains a neutral sphingomyelinase activation domain and a death domain (16). The ECD of mouse TNF RI shows 67%, 70%, 64%, 70% and 88% aa identity with canine, feline, procine, human and rat TNF RI, respectively; and it shows 23% aa identity with the ECD of TNF RII.

目次に戻る

Citations

1. Death-domain dimerization-mediated activation of RIPK1 controls necroptosis and RIPK1-dependent apoptosis
   Authors: H Meng, Z Liu, X Li, H Wang, T Jin, G Wu, B Shan, DE Christoffe, C Qi, Q Yu, Y Li, J Yuan
   Proc. Natl. Acad. Sci. U.S.A., 2018;0(0):.
   Species: Mouse
   Sample Type: Cell Lysates
   Application: WB


2. Endogenous TNF? orchestrates the trafficking of neutrophils into and within lymphatic vessels during acute inflammation
   Authors: S Arokiasamy, C Zakian, J Dilliway, W Wang, S Nourshargh, MB Voisin
   Sci Rep, 2017;7(0):44189.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


3. Sorafenib tosylate inhibits directly necrosome complex formation and protects in mouse models of inflammation and tissue injury
   Authors: S Martens, M Jeong, W Tonnus, F Feldmann, S Hofmans, V Goossens, N Takahashi, JH Bräsen, EW Lee, P Van der Ve, J Joossens, K Augustyns, S Fulda, A Linkermann, J Song, P Vandenabee
   Cell Death Dis, 2017;8(6):e2904.
   Species: Mouse
   Sample Type: Cell Lysates
   Application: IP


4. Anti-inflammatory cytokines directly inhibit innate but not adaptive CD8+ T cell functions.
   Authors: Freeman B, Meyer C, Slifka M
   J Virol, 2014;88(13):7474-84.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


5. Transforming growth factor beta-activated kinase 1 signaling pathway critically regulates myocardial survival and remodeling.
   Authors: Li L, Chen Y, Doan J, Murray J, Molkentin J, Liu Q
   Circulation, 2014;130(24):2162-72.
   Species: Mouse
   Sample Type: Cell Lysates
   Application: WB


6. Therapy of peritoneally disseminated colon cancer by TAP-deficient embryonic stem cell-derived macrophages in allogeneic recipients.
   Authors: Haga E, Endo Y, Haruta M, Koba C, Matsumura K, Takamatsu K, Ikeda T, Nishimura Y, Senju S
   J Immunol, 2014;193(4):2024-33.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


7. Pericytes support neutrophil subendothelial cell crawling and breaching of venular walls in vivo.
   Authors: Proebstl D, Voisin MB, Woodfin A, Whiteford J, D'Acquisto F, Jones GE, Rowe D, Nourshargh S
   J. Exp. Med., 2012;209(6):1219-34.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


8. TNF-alpha drives remodeling of blood vessels and lymphatics in sustained airway inflammation in mice.
   Authors: Baluk P, Yao LC, Feng J, Romano T, Jung SS, Schreiter JL, Yan L, Shealy DJ, McDonald DM
   J. Clin. Invest., 2009;119(10):2954-64.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC Frozen


9. The function of TRADD in signaling through tumor necrosis factor receptor 1 and TRIF-dependent Toll-like receptors.
   Authors: Pobezinskaya YL, Kim YS, Choksi S, Morgan MJ, Li T, Liu C, Liu Z
   Nat. Immunol., 2008;9(9):1047-54.
   Species: Mouse
   Sample Type: Cell Lysates
   Application: IP


10. P2Y1 receptor-evoked glutamate exocytosis from astrocytes: control by tumor necrosis factor-alpha and prostaglandins.
    Authors: Domercq M, Brambilla L, Pilati E, Marchaland J, Volterra A, Bezzi P
    J. Biol. Chem., 2006;281(41):30684-96.
    Species: Rat
    Sample Type: Whole Cells
    Application: ICC

目次に戻る