抗GAPDH [GT239]抗体 | Anti-GAPDH [GT239] antibody

掲載日情報:2020/04/13 現在Webページ番号:194316

GeneTex社の抗GAPDH [GT239]抗体 | Anti-GAPDH [GT239] antibodyです。

本製品は研究用です。研究用以外には使用できません。

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抗GAPDH [GT239]抗体(#GTX627408)のユーザーレビューはこちら。

Ratingrating star full rating star full rating star full rating star full rating star half( Average 4.5 based on 6 users reviews)
Application
Western Blot(WB)rating star full rating star full rating star full rating star full rating star half( Average 4.5 based on 6 users reviews)

特長

  • 高品質の抗体です。
  • 幅広い研究分野に関連する抗体を取り揃えています。
  • 使用されたアプリケーションや動物種などの情報が充実しています。
  • 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

[在庫・価格 :2025年08月01日 16時15分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
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納期 文献数
Anti-GAPDH, Mouse-Mono(GT239)
10日程度 ※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。 445
説明文
レビューあり
別名:glyceraldehyde-3-phosphate dehydrogenase,G3PD,GAPD,HEL-S-162eP
クローン:GT239
Genbank No: 2597
別包装品 別包装品あり
法規制等
保存条件 -20℃ 法規備考
抗原種 Human 免疫動物 Mouse クラス IgG 標識 Unlabeled
交差性 Candida albicans/Chinese Hamster/Dog/Drosophila/E. coli/Hamster/Human/Monkey/Mouse/Pig/Rat/Yeast/Zebrafish 適用 EMSA,IC,IF,IHC,Western Blot
クロナリティ Monoclonal フォーマット 性状 Protein A/G Affinity Purified 吸収処理
掲載カタログ

製品記事 ローディングコントロール抗体
ゼブラフィッシュ(zebrafish)研究用製品特集
使いっきり抗体
抗GAPDH抗体(GeneTex社)
関連記事 GeneTex社における抗体の品質管理
Anti-GAPDH, Mouse-Mono(GT239)
10日程度 ※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。 445
説明文
レビューあり
別名:glyceraldehyde-3-phosphate dehydrogenase,G3PD,GAPD,HEL-S-162eP
クローン:GT239
Genbank No: 2597
別包装品 別包装品あり
法規制等
保存条件 -20℃ 法規備考
抗原種 Human 免疫動物 Mouse クラス IgG 標識 Unlabeled
交差性 Candida albicans/Chinese Hamster/Dog/Drosophila/E. coli/Hamster/Human/Monkey/Mouse/Pig/Rat/Yeast/Zebrafish 適用 EMSA,IC,IF,IHC,Western Blot
クロナリティ Monoclonal フォーマット 性状 Protein A/G Affinity Purified 吸収処理
掲載カタログ

製品記事 ローディングコントロール抗体
ゼブラフィッシュ(zebrafish)研究用製品特集
抗GAPDH抗体(GeneTex社)
関連記事 GeneTex社における抗体の品質管理

[在庫・価格 :2025年08月01日 16時15分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。

Anti-GAPDH, Mouse-Mono(GT239)

文献数: 445

説明文 レビューあり
別名:glyceraldehyde-3-phosphate dehydrogenase,G3PD,GAPD,HEL-S-162eP
クローン:GT239
Genbank No: 2597
別包装品 別包装品あり
法規制等
保存条件 -20℃ 法規備考
抗原種 Human 免疫動物 Mouse
交差性 Candida albicans/Chinese Hamster/Dog/Drosophila/E. coli/Hamster/Human/Monkey/Mouse/Pig/Rat/Yeast/Zebrafish 適用 EMSA,IC,IF,IHC,Western Blot
標識 Unlabeled 性状 Protein A/G Affinity Purified
吸収処理 クラス IgG
クロナリティ Monoclonal フォーマット
掲載カタログ

製品記事 ローディングコントロール抗体
ゼブラフィッシュ(zebrafish)研究用製品特集
使いっきり抗体
抗GAPDH抗体(GeneTex社)
関連記事 GeneTex社における抗体の品質管理

Anti-GAPDH, Mouse-Mono(GT239)

文献数: 445

説明文 レビューあり
別名:glyceraldehyde-3-phosphate dehydrogenase,G3PD,GAPD,HEL-S-162eP
クローン:GT239
Genbank No: 2597
別包装品 別包装品あり
法規制等
保存条件 -20℃ 法規備考
抗原種 Human 免疫動物 Mouse
交差性 Candida albicans/Chinese Hamster/Dog/Drosophila/E. coli/Hamster/Human/Monkey/Mouse/Pig/Rat/Yeast/Zebrafish 適用 EMSA,IC,IF,IHC,Western Blot
標識 Unlabeled 性状 Protein A/G Affinity Purified
吸収処理 クラス IgG
クロナリティ Monoclonal フォーマット
掲載カタログ

製品記事 ローディングコントロール抗体
ゼブラフィッシュ(zebrafish)研究用製品特集
抗GAPDH抗体(GeneTex社)
関連記事 GeneTex社における抗体の品質管理



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DATA IMAGES

Sample (30 μg of whole cell lysate)
A: Drosophila
10% SDS PAGE
GTX627408 diluted at 1:5000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
GAPDH antibody [GT239] detects GAPDH protein by western blot analysis.
A. 30 μg yeast whole cell lysate/extract
10% SDS-PAGE
GAPDH antibody [GT239] (GTX627408) dilution: 1:10000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
GAPDH antibody [GT239] detects GAPDH protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg NIH-3T3 whole cell lysate/extract
C. 30 μg mouse brain lysate/extract
D. 30 μg PC-12 whole cell lysate/extract
E. 30 μg rat brain lysate/extract
10% SDS-PAGE
GAPDH antibody [GT239] (GTX627408) dilution: 1:10000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
GAPDH antibody [GT239] detects GAPDH protein at cytoplasm by immunofluorescent analysis.Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.Green: GAPDH stained by GAPDH antibody [GT239] (GTX627408) diluted at 1:500.Blue: Hoechst 33342 staining.
GAPDH antibody [GT239] detects GAPDH protein by western blot analysis.
A. 20 μg 293T whole cell lysate/extract
B. 10 μg 293T whole cell lysate/extract
C. 5 μg 293T whole cell lysate/extract
D. 1 μg 293T whole cell lysate/extract
10% SDS-PAGE
GAPDH antibody [GT239] (GTX627408) dilution: 1:10000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
The WB analysis of GAPDH antibody [GT239] was published by Yang SH and colleagues in the journal Biomed Res Int in 2014.PMID: 25162007
The WB analysis of GAPDH antibody [GT239] was published by Hammerling BC and colleagues in the journal Nat Commun in 2017.PMID: 28134239
The WB analysis of GAPDH antibody [GT239] was published by Yang SH and colleagues in the journal Biomed Res Int in 2014.PMID: 25162007
The WB analysis of GAPDH antibody [GT239] was published by Chung YH and colleagues in the journal Int J Mol Sci in 2017.PMID: 28829370
The WB analysis of GAPDH antibody [GT239] was published by Yang SH and colleagues in the journal Biomed Res Int in 2014.PMID: 25162007
The WB analysis of GAPDH antibody [GT239] was published by Zhang C and colleagues in the journal Mol Oncol in 2017.PMID: 28100038
GAPDH antibody [GT239] detects GAPDH protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse kidney.GAPDH stained by GAPDH antibody [GT239] (GTX627408) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Various tissue extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GAPDH antibody [GT239] (GTX627408) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
The WB analysis of GAPDH antibody [GT239] was published by Le CT and colleagues in the journal PLoS One in 2018 . PMID: 29444136
The WB analysis of GAPDH antibody [GT239] was published by Al-Sawaf O and colleagues in the journal Sci Rep in 2014 . PMID: 24406502
The WB analysis of GAPDH antibody [GT239] was published by Chou CK and colleagues in the journal Int J Mol Sci in 2015 . PMID: 26151845
The WB analysis of GAPDH antibody [GT239] was published by Chou CK and colleagues in the journal Int J Mol Sci in 2015 . PMID: 26151845
The WB analysis of GAPDH antibody [GT239] was published by Le CT and colleagues in the journal PLoS One in 2018 . PMID: 29444136
The WB analysis of GAPDH antibody [GT239] was published by Garza-Contreras J and colleagues in the journal eNeuro in 2017.PMID: 28856243
The WB analysis of GAPDH antibody [GT239] was published by Hammerling BC and colleagues in the journal Nat Commun in 2017.PMID: 28134239
The WB analysis of GAPDH antibody [GT239] was published by Chung YH and colleagues in the journal Int J Mol Sci in 2017.PMID: 28829370
The WB analysis of GAPDH antibody [GT239] was published by Yang SH and colleagues in the journal Biomed Res Int in 2014.PMID: 25162007
The WB analysis of GAPDH antibody [GT239] was published by Damiani E and colleagues in the journal Sci Rep in 2017.PMID: 28157211
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with (GTX627408) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with GAPDH antibody [GT239] (GTX627408) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.


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製品情報

HostMouse
ClonalityMonoclonal
Clone NameGT239
IsotypeIgG2b
ApplicationWB, ICC/IF, IHC-P, EMSA
ReactivityHuman, Mouse, Rat, Zebrafish, Yeast, Drosophila, Pig, Monkey, E. coli


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APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
EMSA Assay dependent
Not tested in other applications.

Calculated MW 36 kDa. ( Note )
Positive Control 293T , NIH-3T3 , mouse brain , PC-12 , rat brain , E. coli M15 , Drosophila , yeast , zebrafish
Predict Reactivity Rabbit, Sheep, Bovine, Cat, Dog, Chicken, Guinea pig, Xenopus laevis, Rhesus Monkey(>80% identity)


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PROPERTIES

Form Liquid
Buffer PBS
Storage Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration 1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species Human
Immunogen Recombinant protein encompassing a sequence within the center region of human GAPDH. The exact sequence is proprietary.
Purification Affinity purified by Protein G.
Conjugation Unconjugated
Note For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.


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TARGET

Synonyms glyceraldehyde-3-phosphate dehydrogenase , G3PD , GAPD , HEL-S-162eP
Cellular Localization Cytoplasm , perinuclear region , Membrane
Background The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Many pseudogenes similar to this locus are present in the human genome. [provided by RefSeq]
Database ・ Gene ID: 2597 GAPDH
・ UniProt: P04406 GAPDH


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REFERENCE

Application ReferenceApplication/Reactivity
Baltes F et al. Biochim Biophys Acta Mol Cell Res 2020; 1867 (5) : 118663 β1-Integrin binding to collagen type 1 transmits breast cancer cells into chemoresistance by activating ABC efflux transporters.Application : WB
Reactivity : Human
Sarkar A et al. Haematologica 2020; (Epub) Ataxia telangiectasia mutated interacts with Parkin and induces mitophagy independent of kinase activity. Evidence from mantle cell lymphoma.Application : WB
Reactivity : Human
Dragan M et al. Cell Death Dis 2020; 11 (2) : 106 G protein-coupled kisspeptin receptor induces metabolic reprograming and tumorigenesis in estrogen receptor-negative breast cancer.Application : WB
Reactivity : Human
Wangta Liu et al. Oxidative Medicine and Cellular Longevity 2020; 2020 : Article ID 5167292 The Phenoxyphenol Compound 4-HPPP Selectively Induces Antiproliferation Effects and Apoptosis in Human Lung Cancer Cells through Aneupolyploidization and ATR DNA Repair Signaling.Application : WB
Reactivity : Human
Robertson R et al. Am J Pathol 2020; (Epub) BAG3P215L/KO Mice as a Model of BAG3P209L Myofibrillar Myopathy.Application : WB
Reactivity : Mouse


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