抗NF-M抗体 | Anti-NF-M antibody

• 特長
• 価格
• DATA IMAGES
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

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DATA IMAGES

	Various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with NF-M antibody (GTX133218) diluted at 1:1000.
	NF-M antibody detects NF-M protein at nucleus by immunofluorescent analysis.Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.Green: NF-M protein stained byNF-M antibody (GTX133218) diluted at 1:500.Red: beta Tubulin 3/ Tuj1, a neuron cell marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
	NF-M antibody detects NF-M protein expression by immunohistochemical analysis.Sample: Frozen-sectioned adult mouse hippocampus. Green: NF-M protein stained by NF-M antibody (GTX133218) diluted at 1:250.Red: NeuN, stained by NeuN antibody [2Q158] (GTX30773) diluted at 1:500.
	NF-M antibody detects NF-M protein expression by immunohistochemical analysis.Sample: Frozen-sectioned adult mouse cerebellum. Green: NF-M protein stained by NF-M antibody (GTX133218) diluted at 1:250.Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).

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製品情報

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-Fr
Reactivity	Mouse, Rat

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APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-Fr	1:100-1:1000

Not tested in other applications.

Calculated MW	96 kDa. ( Note )
Positive Control	mouse brain , rat brain
Predict Reactivity	Human, Bovine, Chicken, Xenopus laevis(>80% identity)

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PROPERTIES

Form	Liquid
Buffer	1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration	0.1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species	Mouse
Immunogen	Recombinant protein encompassing a sequence within the C-terminus region of mouse NF-M. The exact sequence is proprietary.
Purification	Purified by antigen-affinity chromatography.
Conjugation	Unconjugated
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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TARGET

Synonyms	neurofilament, medium polypeptide , NF-M , NF160 , NF165 , Nef3 , Nfm
Background	Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber.
Database	・ Gene ID: 18040 Nefm ・ UniProt: P08553 Nefm

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