抗Histone H3K56ac (Acetyl Lys56) [RM179]抗体 | Anti-Histone H3K56ac (Acetyl Lys56) [RM179] antibody

• 特長
• 価格
• DATA IMAGES
• 製品情報
• APPLICATION
• PROPERTIES
• REFERENCEE(抜粋)

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

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DATA IMAGES

	Immunocytochemistry of HeLa cells treated with sodium butyrate, using Acetyl-Histone H3 (Lys56) Rabbit mAb RM179 (red). Actin filaments have been labeled with fluorescein phalloidin (green).
	RM179 specifically reacts to Histone H3 acetylated at Lysine 56(K56ac). No cross reactivity with unmodified Lysine 56 (K56 ctrl),acetylated Lysine 4 (K4ac), Lysine 9 (K9ac), Lysine 14 (K14ac),Lysine 18 (K18ac), Lysine 23 (K23ac), Lysine 27 (K27ac), Lysine 36 (K36ac), lysine 79 (K79ac), or Lysine 122 (K122) in histone H3.
	Western Blot of (1) acid extracts of HeLa cells treated withsodium butyrate and (2) recombinant histone H3.3, usingRM179 at 1 μg/mL, showed a band of histone H3 acetylatedat Lysine 56 in treated HeLa.

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製品情報

Host	Rabbit
Clonality	Monoclonal
Clone Name	RM179
Isotype	IgG
Application	WB, ICC/IF, ELISA, ChIP assay, IHC, Multiplexing
Reactivity	Human

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APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	Assay dependent
ICC/IF	Assay dependent
ELISA	Assay dependent
ChIP assay	Assay dependent
IHC	Assay dependent
Multiplexing	Assay dependent

Not tested in other applications.

Specificity/Sensitivity	This antibody reacts to Histone H3 acetylated at Lysine 56 (K56ac). No cross reactivity with other acetylated Lysines in histone H3.

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PROPERTIES

Form	Liquid
Buffer	PBS with 50% Glycerol and 1% BSA. 0.09% Sodium azide was added as a preservative.
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration	1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species	Human
Immunogen	An acetyl-peptide corresponding to Acetyl-Histone H3 (Lys56).
Purification	Protein A affinity purified from an animal origin–free culture supernatant
Conjugation	Unconjugated
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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REFERENCEE(抜粋)

Application Reference	Application/Reactivity
Zhu Q et al. Oncotarget 2017; 8 (61) : 104525-104542 Human CRL4DDB2 ubiquitin ligase preferentially regulates post-repair chromatin restoration of H3K56Ac through recruitment of histone chaperon CAF-1.	Application : WB Reactivity : Human

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