抗Histone H3K14me2 (Di-methyl Lys14) [RM165]抗体 | Anti-Histone H3K14me2 (Di-methyl Lys14) [RM165] antibody

• 特長
• 価格
• DATA IMAGES
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

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DATA IMAGES

	RM165 specifically reacts to Histone H3 dimethylatedat Lysine 14 (K14me2). No cross reactivity with non-modified Lysine 14 (H3K14), monomethylated Lysine14 (K14me1), trimethylated Lysine 14 (K14me3), or other methylations in Histone H3.
	Western Blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2), using RM165 at 0.25 ug/mL, showed a band of histone H3 dimethylated at Lysine 14 (K14me2) in HeLa cells.

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製品情報

Host	Rabbit
Clonality	Monoclonal
Clone Name	RM165
Isotype	IgG
Application	WB, ELISA, Multiplexing
Reactivity	Human

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APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	Assay dependent
ELISA	Assay dependent
Multiplexing	Assay dependent

Not tested in other applications.

Specificity/Sensitivity	This antibody reacts to Histone H3 dimethylated at Lysine 14 (K14me2). No cross reactivity with monomethylated Lysine 14 (K14me1) or trimethylated Lysine 14 (K14me3), or other methylation in histone H3.

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PROPERTIES

Form	Liquid
Buffer	PBS with 50% Glycerol and 1% BSA. 0.09% Sodium azide was added as a preservative.
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration	1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species	Human
Immunogen	A dimethyl-peptide corresponding to Dimethyl-Histone H3 (Lys14).
Purification	Protein A affinity purified from an animal origin–free culture supernatant
Conjugation	Unconjugated
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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TARGET

Synonyms

H3/A Antibody , HIST1H3A Antibody , H3FA Antibody , histone cluster 1, H3a Antibody , H3K14Me2 antibody

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Jul 2008]

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