抗Histone H3K79ac (Acetyl Lys79) [RM156]抗体 | Anti-Histone H3K79ac (Acetyl Lys79) [RM156] antibody

• 特長
• 価格
• DATA IMAGES
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

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DATA IMAGES

	Western Blot of acid extracts from HeLa cells untreated (-) or treated with sodium butyrate (+), using RM156 at 1.0 ug/mL, showed a band of histone H3 acetylated at Lysine 79 in treated HeLa.
	ELISA analysis of HeLa whole cell lysate treated or untreated with Sodium Butyrate using GTX60883 Histone H3K79ac (Acetyl Lys79) antibody [RM156] (5 ug/mL) as capture antibody and GTX60888 Histone H3 antibody [RM188] (1 ug/mL) as detection antibody.
	RM156 specifically reacts to Histone H3 acetylated at Lysine 79 (K79ac). No cross reactivity with acetylated Lysine 4 (K4ac), Lysine 9 (K9ac), Lysine 14 (K14ac), Lysine 18 (K18ac), Lysine 23 (K23ac), Lysine 27 (K27ac), Lysine 36 (K36ac), Lysine 56 (K56ac), or Lysine 122 (K122) in Histone H3.
	Immunocytochemistry of HeLa cells treated with sodium butyrate, using Acetyl-Histone H3 (Lys79) Rabbit mAb RM156 (red). Actin filaments have been labeled with fluorescein phalloidin (green).
	ChIP performed on HeLa cells with or without Sodium Butyrate treatment, using H3K79Ac antibody (RM156, 5ug). Real-time PCR was performed using primers specific to the geneindicated.

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製品情報

Host	Rabbit
Clonality	Monoclonal
Clone Name	RM156
Isotype	IgG
Application	WB, ICC/IF, ELISA, ChIP assay, Multiplexing, sELISA
Reactivity	Human

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APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	Assay dependent
ICC/IF	Assay dependent
ELISA	Assay dependent
ChIP assay	Assay dependent
Multiplexing	Assay dependent
sELISA	Assay dependent
Note : sELISA Capture : GTX60883, Detection : GTX60888

Not tested in other applications.

Specificity/Sensitivity	This antibody reacts to Histone H3 acetylated at Lysine 79 (K79ac). No cross reactivity with other acetylated Lysines in Histone H3.

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PROPERTIES

Form	Liquid
Buffer	PBS with 50% Glycerol and 1% BSA. 0.09% Sodium azide was added as a preservative.
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration	1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species	Human
Immunogen	An acetyl-peptide corresponding to Acetyl-Histone H3 (Lys79).
Purification	Protein A affinity purified from an animal origin–free culture supernatant
Conjugation	Unconjugated
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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TARGET

Synonyms

histone cluster 1, H3a Antibody , H3FA Antibody , HIST1H3A Antibody , H3/A Antibody , H3K79Ac antibody

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Jul 2008]

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