抗Histone H3K4me3 (tri-methyl Lys4)抗体 | Anti-Histone H3K4me3 (tri-methyl Lys4) antibody

• 特長
• 価格
• DATA IMAGES
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

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DATA IMAGES

	ICC/IF analysis of 293T cells using GTX55482 Histone H3K4me3 (tri-methyl Lys4) antibody.Blue : DAPI
	ChIP analysis of 293 cells using GTX55482 Histone H3K4me3 (tri-methyl Lys4) antibody. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
	WB analysis of various sample lysates using GTX55482 Histone H3K4me3 (tri-methyl Lys4) antibody.Loading : 25ug per lane
	IHC-P analysis of rat brain tissue using GTX55482 Histone H3K4me3 (tri-methyl Lys4) antibody. Dilution : 1:200
	Dot blot analysis of various methylation peptides using GTX55482 Histone H3K4me3 (tri-methyl Lys4) antibody. Dilution : 1:1000
	IHC-P analysis of human rectal cancer tissue using GTX55482 Histone H3K4me3 (tri-methyl Lys4) antibody. Dilution : 1:200

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製品情報

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, IP, Dot, ChIP assay
Reactivity	Human, Mouse, Rat, Rice, Zea mays

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APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1:500-1:2000
ICC/IF	1:50-1:200
IHC-P	1:50-1:200
IP	1:50-1:200
Dot	Assay dependent
ChIP assay	1:20-1:100

Not tested in other applications.

Calculated MW	16 kDa. ( Note )

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PROPERTIES

Form	Liquid
Buffer	PBS pH7.3, 0.02% sodium azide, 50% glycerol.
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration	Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species	Human
Immunogen	A synthetic methylated peptide corresponding to residues surrounding K4 of human histone H3
Purification	Purified by affinity chromatography
Conjugation	Unconjugated
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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TARGET

Synonyms	histone cluster 3 H3 , H3.4 , H3/g , H3FT , H3t
Cellular Localization	Nucleus
Background	Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015]
Database	・ Gene ID: 8290 HIST3H3 ・ UniProt: Q16695 HIST3H3

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