抗PAX5抗体 | Anti-PAX5 antibody

• 特長
• 価格
• DATA IMAGES
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

目次に戻る

価格

目次に戻る

DATA IMAGES

	ICC/IF analysis of Ramos cells using GTX54532 PAX5 antibody. Cells were probed without (left) or with(right) an antibody.Green : Primary antibodyBlue : NucleiFixation : FormalinPermeabilization : 0.1% Triton X-100 in TBS for 5-10 minuteDilution : 1:100 and incubated overnight at 4ºC
	WB analysis of 75μg of whole cell lysates from PAX5-expressing B cell lymphoma-derived cell lines, negative control Jurkat cells using GTX54532 PAX5 antibody. Dilution : 1:1000
	ICC/IF analysis of Raji cells using GTX54532 PAX5 antibody. Cells were probed without (left) or with(right) an antibody.Green : Primary antibodyBlue : NucleiFixation : FormalinPermeabilization : 0.1% Triton X-100 in TBS for 5-10 minuteDilution : 1:100 and incubated overnight at 4ºC
	ICC/IF analysis of Ramos and negative control Jurkat cells using GTX54532 PAX5 antibody. Green : Primary antibodyBlue : NucleiRed : ActinFixation : FormalinPermeabilization : 0.1% Triton X-100 in TBS for 10 minutesDilution : 1:100 for at least 1 hour at room temperature
	IHC-P analysis of human tonsil tissue using GTX54532 PAX5 antibody. Right : Primary antibodyLeft : Negative control without primary antibodyAntigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 minDilution : 1:200
	IP analysis of Raji cell lysates using GTX54532 PAX5 antibody. IP reaction : 2μg antibody / 750μg lysateDilution : 1:1000
	IHC-P analysis of mouse lymph node tissue using GTX54532 PAX5 antibody. Right : Primary antibodyLeft : Negative control without primary antibodyAntigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 minDilution : 1:100
	FACS analysis of permeabilized Ramos cells (red histogram) or PBS alone control(black histogram) using GTX54532 PAX5 antibody. Fixation : 4% formaldehydeDilution : 1:50

目次に戻る

製品情報

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, FACS, IP
Reactivity	Human, Mouse

目次に戻る

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1:500-1:2000
ICC/IF	1:20-1:200
IHC-P	1:50-1:500
FACS	1:50
IP	2-5 μg

Not tested in other applications.

Calculated MW	42 kDa. ( Note )

目次に戻る

PROPERTIES

Form	Liquid
Buffer	PBS, 1mg/ml BSA, 30% glycerol, 0.05% sodium azide
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration	1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species	Human
Immunogen	Purified internal fragment of human recombinant PAX5 expressed in E. coli
Purification	Protein G purified
Conjugation	Unconjugated
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

目次に戻る

TARGET

Synonyms	paired box 5 , ALL3 , BSAP
Cellular Localization	Nucleus
Background	This gene encodes a member of the paired box (PAX) family of transcription factors. The central feature of this gene family is a novel, highly conserved DNA-binding motif, known as the paired box. Paired box transcription factors are important regulators in early development, and alterations in the expression of their genes are thought to contribute to neoplastic transformation. This gene encodes the B-cell lineage specific activator protein that is expressed at early, but not late stages of B-cell differentiation. Its expression has also been detected in developing CNS and testis and so the encoded protein may also play a role in neural development and spermatogenesis. This gene is located at 9p13, which is involved in t(9;14)(p13;q32) translocations recurring in small lymphocytic lymphomas of the plasmacytoid subtype, and in derived large-cell lymphomas. This translocation brings the potent E-mu enhancer of the IgH gene into close proximity of the PAX5 promoter, suggesting that the deregulation of transcription of this gene contributes to the pathogenesis of these lymphomas. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2013]
Database	・ Gene ID: 5079 PAX5 ・ UniProt: Q02548 PAX5

目次に戻る